Library Science

Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask–and answer–all the important questions emerging from the dramatically advancing work in vision research today. Ed. Rakoczy, P. Elizabeth

Table of Contents

1. Basic Molecular Biology Techniques (pp. 1-30)
   Lai, Chooi-May
2. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes (pp. 31-43)
   Farber, Debora B.; Lerner, Leonid E.; Viczian, Andrea S.
3. In Situ Hybridization (pp. 45-69)
   Yang, Xian-Jie
4. Immunocytochemical Studies of the Retina (pp. 71-88)
   Milam, Ann H.
5. Antisense DNA Technology (pp. 89-104)
   Rakoczy, Piroska Elizabeth
6. Inhibition of Gene Expression by Ribozymes William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, (pp. 105-124)
   White, D. Alan; Lewin, Alfred S.
7. Intraocular Delivery of Recombinant Virus (pp. 125-139)
   Liang, Fong-Qi; Anand, Vibha; Maguire, Albert M.; Bennett, Jean
8. Preparation of Recombinant Adenoviruses (pp. 141-155)
   Csaky, Karl G.
9. Generation of Recombinant Adeno-Associated Virus (pp. 157-170)
   Rolling, Fabienne
10. Preparation of Recombinant Retroviruses (pp. 171-190)
    Yang, Xian-Jie
11. Generation of Transgenic Mice for Studies of Ocular Development and Disease (pp. 191-214)
    Gupta, Abha R.; Dejneka, Nadine S.; Maguire, Albert M.; Bennett, Jean
12. Generation of Knockout Animal Models (pp. 215-236)
    Redmond, T. Michael
13. Mutation Analysis of Ocular Genes (pp. 237-250)
    Danciger, Michael
14. Ophthalmologists??? Perspective (pp. 251-305)
    Downes, Susan M.; Chong, N. H. Victor; Bird, Alan C.

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