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Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

Authors: M. H. Hofker and J. van Deursen

transgenic-mouse.pdf Table of Contents

1. Introduction: The Use of Transgenic Mice in Biomedical Research (pp. 01-08)
   Hofker, Marten H.
2. Genetic Modifi cation of the Mouse: General Technology; Pronuclear and Blastocyst Injection (pp. 09-34)
   Voncken, J. Willem
3. In Vitro Fertilization/Cryopreservation (pp. 35-50)
   Marschall, Susan; de Angelis, Martin Hrabe
4. Transgene Design (pp. 51-68)
   Voncken, J. Willem
5. Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression (pp. 69-104)
   Schonig, Kai; Bujard, Hermann
6. Manipulating Large Insert Clones for Transgenesis (pp. 105-124)
   McCormick, Sally P. A.; Liu, Catherine Y. Y.; Young, Stephen G.; Nielsen, Lars B.
7. Reporter Molecules in Genetically Engineered Mice (pp. 125-144)
   Saunders, Thomas L.
8. Gene Targeting in Mouse Embryonic Stem Cells (pp. 145-158)
   van Deursen, Jan
9. Conditional Knockout Mice (pp. 159-186)
   Kuhn, Ralf; Schwenk, Frieder
10. Knockin Approaches (pp. 187-200)
    Roebroek, Anton J. M.; Wu, Xiaosheng; Bram, Richard J.
11. Aggregation of Embryos and Embryonic Stem Cells (pp. 201-230)
    Maatman, Ronald; Gertsenstein, Marina; Meijer, Emile de; Nagy, Andras; Vintersten, Kristina
12. Adenovirus-Mediated Gene Transfer (pp. 231-248)
    van Dijk, Ko Willems; Kypreos, Kyriakos E.; DOliveira, Christine; Fallaux, Frits J.
13. Random ENU Mutagenesis (pp. 249-266)
    Soewarto, Dian; Blanquet, Veronique; de Angelis, Martin Hrabe
14. Autopsy and Histologic Analysis of the Transgenic Mouse (pp. 267-280)
    Gijbels, Marion J. J.; de Winther, Menno P. J.
15. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells (pp. 281-292)
    de Winther, Menno P. J.; Heeringa, Peter
16. Quantification of Atherosclerosis in Mice (pp. 293-310)
    Daugherty, Alan; Whitman, Stewart C.
17. Genetically Modified Mice in Cancer Research (pp. 311-332)
    Largaespada, David A.
18. Neuropathobiology in Transgenic Mice: The Case of Alzheimers Disease (pp. 333-362)
    Dorpe, Jo Van; Spittaels, Kurt; den Haute, Chris Van; Dewachter, Ilse; Moechars, Dieder; Geerts, Hugo; Leuven, Fred Van

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