Library Science

Practical methods for the generation and analysis of transgenic animals, covering a comprehensive range of species, from invertebrates to lower vertebrates to higher vertebrates. Chapters examine ways in which transgenic techniques can be used to address many problems in biological systems present in-depth descriptions of analytical methods, and give step-by-step techniques that can be easily performed in your lab.

Authors: Murphy, David; Carter, David A.

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Table of Contents

1. Introduction to Transgenesis (pp. 3-6)
   Murphy, David; Carter, David A.
2. Transgenic Rodents and the Study of the Central Nervous System (pp. 7-22)
   Carter, David A.
3. Transgenic Animals and the Study of Cancer (pp. 23-36)
   Murphy, David
4. Transgenic Animals and the Study of the Immune System (pp. 37-52)
   Hui, Kam M.
5. The Commercial and Agricultural Applications of Animal Transgenesis (pp. 53-71)
   Ward, Kevin A.; Nancarrow, Colin D.
6. P Element-Mediated Germ-Line Transformation of Drosophila (pp. 75-86)
   O???Connor, Mark; Chia, William
7. Gene Transfer by Microinjection in the Zebra Fish Brachydanio rerio (pp. 87-94)
   Khoo, Hong-Woo; Ang, Lay-Hong; Lim, Hueh-Bin
8. Transgenic Induction in Salmonid and Tilapia Fish (pp. 95-107)
   Maclean, Norman
9. An Overview of Transgenic Mouse Production (pp. 111-114)
   Murphy, David; Carter, David A.
10. Transgene Design (pp. 115-118)
    Murphy, David; Carter, David A.; Smith, Duncan R.
11. Isolation of DNA Fragments for Microinjection (pp. 119-124)
    Smith, Duncan R.; Murphy, David
12. Establishing a Colony for Efficient Production of Transgenic Mice (pp. 125-130)
    Carter, David A.
13. Mating Mice (pp. 131-134)
    Murphy, David
14. Anesthetizing Mice (pp. 135-136)
    Carter, David A.
15. Vasectomizing a Mouse (pp. 137-140)
    Murphy, David
16. Preparation of Culture Media for Fertilized One-Cell Mouse Eggs (pp. 141-144)
    Carter, David A.
17. Collection of Fertilized One-Cell Mouse Eggs for Microinjection (pp. 145-150)
    Carter, David A.
18. Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs: I. Manual Injection (pp. 151-162)
    Murphy, David
19. Microinjection of Cloned DNA Fragments into Fertilized One-Cell Mouse Eggs: II. Automatic Injection (pp. 163-168)
    Murphy, David
20. Delivery of Microinjected Eggs to Surrogate Mothers by Oviduct Transfer (pp. 169-176)
    Carter, David A
21. Caesarean Section and Fostering (pp. 177-178)
    Murphy, David
22. In vitro Fertilization of Mouse Eggs (pp. 179-182)
    Carter, David A.
23. The In Vitro Isolation of Murine Embryonic Stem Cells (pp. 183-216)
    Wells, Dave
24. Production of Chimeras Derived from Murine Embryonic Stem Cells (pp. 217-238)
    Wells, Dave
25. Cryopreservation of Transgenic Mouse Lines (pp. 239-243)
    Pomeroy, Kimball O.
26. Introduction to the Physiology and Husbandry of the Rat (pp. 247-252)
    Phang, Chiew-Hun
27. Mating of Rats (pp. 253-256)
    Phang, Chiew-Hun
28. Vasectomy of the Male Rat (pp. 257-260)
    Phang, Chiew-Hun
29. Harvest of Fertilized One-Cell Rat Eggs (pp. 261-264)
    Phang, Chiew-Hun
30. Implantation of Microinjected Eggs (pp. 265-268)
    Phang, Chkw-Hun
31. Cryopreservation of Transgenic Rat Lines (pp. 269-272)
    Pomeroy, Kimball O.
32. Production of Transgenic Sheep (pp. 273-304)
    Nancarrow, Colin D.; Marshall, James T. A.; Ward, Kevin A.
33. Cryopreservation of Transgenic Sheep Lines (pp. 305-306)
    Pomeroy, Kimball O.
34. Isolation of Genomic DNA from Tail Tissue (pp. 309-312)
    Murphy, David
35. Slot Blotting of Genomic Tail DNA (pp. 313-316)
    Murphy, David
36. Genomic Analysis of Transgenic Animals by the Polymerase Chain Reaction (pp. 317-322)
    Abbott, Cathy
37. Genomic Analysis of Transgenic Animals: Southern Blotting (pp. 323-327)
    Smith, Duncan R.; Murphy, David
38. The Preparation of RNA from the Tissues of Transgenic Animals (pp. 331-336)
    Murphy, David
39. Northern Blotting (pp. 337-340)
    Murphy, David
40. RNA Slot Blotting (pp. 341-344)
    Murphy, David
41. Analysis of Gene Expression by PCR (pp. 345-354)
    Koopman, Peter
42. Nuclear Run-On Analysis of Transcription (pp. 355-362)
    Murphy, David
43. S1 Nuclease Protection Mapping (pp. 363-372)
    Smith, Duncan R.
44. Primer Extension (pp. 373-378)
    Smith, Duncan R.
45. In Situ Hybridization Analysis of Transgenic Tissues (pp. 379-394)
    Funkhouser, Judith McNiff
46. Immunohistochemical Analysis of Transgene Expression (pp. 395-406)
    Funkhouser, Judith McNiff
47. Postmortem Examination of Transgenic Mice (pp. 407-412)
    Chooi, Kum Fai
48. Histological Examination of Transgenic Mice (pp. 413-418)
    Chooi, Kum Fai
49. Reporter Enzyme Assays (pp. 419-424)
    Pardy, Karen
50. Restriction Endonuclease Digestion of DNA (pp. 427-432)
    Smith, Duncan R.
51. Agarose Gel Electrophoresis (pp. 433-438)
    Smith, Duncan R.
52. Working with Radioactivity in the Transgenic Animal Laboratory (pp. 439-444)
    Smith, Duncan R.
53. Random Primed Labeling of DNA (pp. 445-448)
    Smith, Duncan R.
54. Filter Hybridization (pp. 449-452)
    Murphy, David
55. DNA Transfection (pp. 453-456)
    Pardy, Karen

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