Library Science

The first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered. Ed. Morgan, Jeffrey R. ; Yarmush, Martin L.

Table of Contents

1. Preparation of Collagen?Glycosaminoglycan Copolymers for Tissue Regeneration (pp. 3-18)
   Chamberlain, Lila J.; V.Yannas, loannis
2. Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology (pp. 19-34)
   Sofia, Susan J.; Kuhl, Philip R.; Griffith, Linda G.
3. Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering (pp. 35-46)
   Garrido, Leoncio
4. Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering (pp. 47-56)
   Ma, Peter X.; Langer, Robert
5. Formation of Highly Porous Polymeric Foams with Controlled Release Capability: A Phase-Separation Technique (pp. 57-66)
   Kadiyala, S.; Lo, H.; Leong, K. W.
6. Magnetic-induced Alignment of Collagen Fibrils in Tissue Equivalents (pp. 67-74)
   Girton, Timothy S.; Dubey, Narendra; Tranquillo, Robert T.
7. Preparation and Use of Thermosensitive Polymers (pp. 75-84)
   Cao, Yi-Lin; Ibarra, Clemente; Vacanti, Charles
8. ln Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing (pp. 85-100)
   Sawhney, Amarpreet S.; Hubbell, Jeffrey A.
9. Fabrication and Implantation of Gel-Filled Nerve Guidance Channels (pp. 101-120)
   Bellamkonda, Ravi V.; Valentini, Robert F.
10. Small Animal Surgical and Histological Procedures for Characterizing the Performance of Tissue-Engineered Bone Grafts (pp. 121-132)
    James, Kenneth S.; Zimmerman, Mark C.; Kohn, Joachim
11. Preparation and Use of Porous Poly(a-Hydroxyester) Scaffolds for Bone Tissue Engineering (pp. 133-140)
    Jen, Anna C.; Peter, Susan J.; Mikos, Antonios G.
12. Quantitative Assessment of Autocrine Cell Loops (pp. 143-154)
    Oehrtman, Gregory; Walker, Laura; Will, Birgit; Opresko, Lee; Wiley, H. Steven; Lauffenburger, Douglas A.
13. Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy (pp. 155-172)
    Faulkner, John A.; Brooks, Susan V.; Dennis, Robert G.
14. Preparation of Immortalized Human Chondrocyte Cell Lines (pp. 173-192)
    Robbins, James R.; Goldring, Mary B.
15. Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells (pp. 195-204)
    Shortkroff, Sonya; Spector, Myron
16. Culture and Identification of Autologous Human Articular Chondrocytes for Implantation (pp. 205-216)
    Tubo, Ross; Binette, Francois
17. Organogenesis of Skeletal Muscle in Tissue Culture (pp. 217-226)
    Vandenburgh, Herman; Shansky, Janet; Del Tatto, Michael; Chromiak, Joseph
18. Hepatocyte Primary Cultures: Currently Used Systems and Their Applications for Studies of Hepatocyte Growth and Differentiation (pp. 227-244)
    Michalopoulos, George K.
19. Formation and Characterization of Hepatocyte Spheroids (pp. 245-252)
    Friend, Julie R.; Wu, Florence J.; Hansen, Linda K.; Remmel, Rory P.; Hu, Wei-Shou
20. Isolation and Culture of Human Endothelial Cells (pp. 253-260)
    Berthiaume, Francois
21. Isolation and Culture of Microvascular Endothelial Cells (pp. 261-270)
    Richard, Lisa; Velasco, Paula; Detmar, Michael
22. Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures (pp. 271-292)
    Collins, Paul C.; Patel, Sanjay D.; Miller, William M.; Papoutsakis, E. Terry
23. Methods to Isolate, Culture, and Study Osteoblasts (pp. 293-302)
    Hillsley, Mechteld V.
24. Cryopreservation of Rat Hepatocytes in a Three- Dimensional Culture Configuration Using a Controlled-Rate Freezing Device (pp. 303-312)
    Russo, Michael J.; Toner, Mehmet
25. Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms (pp. 315-330)
    Chang, Thomas M. S.
26. Methods for Microencapsulation with HEMA-MMA (pp. 331-348)
    Lahooti, Shahab; Sefton, Michael V.
27. Micropatterning Cells in Tissue Engineering (pp. 349-364)
    Bhatia, Sangeeta N.; Yarmush, Martin L.; Toner, Mehmet
28. Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen?GAG-Based Skin Substitutes (pp. 365-390)
    Boyce, Steven T.
29. Use of Skin Equivalent Technology in a Wound Healing Model (pp. 391-406)
    Vaccariello, Michael A.; Javaherian, Ashkan; Parenteau, Nancy; Garlick, Jonathan A.
30. Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis (pp. 407-422)
    Medalie, Daniel A.; Morgan, Jeffrey R.
31. Development of a Bioartificial Liver Device (pp. 423-432)
    Hansen, Linda K.; Friend, Julie R.; Remmel, Rory; Cerra, Frank B.; Hu, Wei-Shou
32. Methods for the Implantation of Liver Cells (pp. 433-446)
    Kim, Stephen S.; Utsunomiya, Hirofumi; Vacanti, Joseph P.
33. Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture (pp. 447-456)
    Berthiaume, Francois; Tompkins, Ronald G.; Yarmush, Martin L.
34. Design and Fabrication of a Small Caliber Hybrid Arterial Bioprosthesis (pp. 457-468)
    L?ltalien, Gilbert J.; Abbott, William M.
35. Methods for the lmmunoisolation and Transplantation of Pancreatic Cells (pp. 469-482)
    Sun, Anthony M.
36. Methods for the Study of Nerve Cell Migration and Patterning (pp. 483-493)
    Buettner, Helen M.; Tai, Hsin-Chien
37. Estimating Number and Volume of Islets Transplanted Within a Planar lmmunobarrier Diffusion Chamber (pp. 497-506)
    Suzuki, Kazuhisa; Colton, Clark K.; Bonner-Weir, Susan; Hollister, Jennifer; Weir, Gordon C.
38. Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions (pp. 507-520)
    Ramos, Carroll L.; Lawrence, Michael 6.
39. Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation (pp. 521-542)
    Allen, R. Gregory; Eisenberg, Solomon R.; Gray, Martha L.
40. Measuring Receptor-Mediated Cell Adhesion Under Flow: Cell-Free Systems (pp. 543-552)
    Hammer, Daniel A.; Brunk, Debra K.
41. In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium (pp. 553-576)
    Jain, Rakesh K.; Munn, Lance L.; Fukumura, Dai; Melder, Robert J.
42. Quantitative Measurement of Shear-Stress Effects on Endothelial Cells (pp. 577-594)
    Papadaki, Maria; Mclntire, Larry V.
43. Quantitative Modeling of Limitations Caused by Diffusion (pp. 595-606)
    Sambanis, Athanassios; A.Tan, Sanda

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