Library Science

A first-time collection of cutting-edge morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood???brain barrier in health and disease. Described in step-by-step detail, each readily reproducible method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technqiue. These powerful tools can be used to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.

Authors: Nag, Sukriti

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Table of Contents

1. Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels (pp. 3-36)
   Nag, Sukriti
2. Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry (pp. 37-50)
   Nag, Sukriti
3. Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions (pp. 51-66)
   Wolburg, Hartwig; Liebner, Stefan; Lippoldt, Andrea
4. Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy (pp. 67-82)
   Lossinsky, Albert S.; Shivers, Richard R.
5. Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy (pp. 83-94)
   Engelhardt, Britta; Vajkoczy, Peter; Laschinger, Melanie
6. Pathophysiology of Blood???Brain Barrier Breakdown (pp. 97-120)
   Nag, Sukriti
7. Pial Vessel Permeability to Tracers Using Cranial Windows (pp. 121-132)
   Mayhan, William G.
8. Blood???Brain Barrier Permeability Using Tracers and Immunohistochemistry (pp. 133-144)
   Nag, Sukriti
9. Ultracytochemical Studies of the Compromised Blood???Brain Barrier (pp. 145-160)
   Nag, Sukriti
10. Immunogold Detection of Microvascular Proteins in the Compromised Blood???Brain Barrier (pp. 161-176)
    Cornford, Eain M.; Hyman, Shigeyo; Cornford, Marcia E.
11. Measurement of Blood???Brain and Blood???Tumor Barrier Permeabilities with [14C]-Labeled Tracers (pp. 177-190)
    Asotra, Kamlesh; Ningaraj, Nagendra; Black, Keith L.
12. A Review of Blood???Brain Barrier Transport Techniques (pp. 193-208)
    Smith, Quentin R.
13. In Situ Brain Perfusion Technique (pp. 209-218)
    Smith, Quentin R.; Allen, David D.
14. Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood???Brain Barrier (pp. 219-232)
    Kusuhara, Hiroyuki; Terasaki, Tetsuya; Sugiyama, Yuichi
15. Transport Studies Using Membrane Vesicles (pp. 233-248)
    Peterson, Darryl R.; Hawkins, Richard A.
16. Drug Transport Studies Using Quantitative Microdialysis (pp. 249-264)
    Dai, Haiqing; Elmquist, William F.
17. Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids (pp. 265-276)
    Puchowicz, Michelle A.; Xu, Kui; LaManna, Joseph C.
18. Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin (pp. 277-290)
    Fenart, Laurence; Cecchelli, Romeo
19. Transport Across the Choroid Plexuses In Vivo and In Vitro (pp. 291-304)
    Strazielle, Nathalie; Preston, Jane Elizabeth
20. An Overview of In Vitro Techniques for Blood-Brain Barrier Studies (pp. 307-324)
    Reichel, Andreas; Begley, David J.; Abbott, N. Joan
21. Isolation and Characterization of Human Brain Endothelial Cells (pp. 325-336)
    Dorovini-Zis, Katerina; Prameya, Rukmini; Huynh, Hanh
22. Human Brain Microvessel Endothelial Cell and Leukocyte Interactions (pp. 337-348)
    Shukaliak-Quandt, Jacqueline; Wong, Donald; Dorovini-Zis, Katerina
23. Development and Characterization of Immortalized Cerebral Endothelial Cell Lines (pp. 349-364)
    Couraud, Pierre-Olivier; Greenwood, John; Roux, Francoise; Adamson, Pete
24. Isolation and Characterization of Retinal Endothelial Cells (pp. 365-374)
    Antonetti, David A.; B.Wolpert, Ellen
25. Isolation and Characterization of Cerebral Microvascular Pericytes (pp. 375-382)
    Dore-Duffy, Paula
26. Molecular Biology of the Blood???Brain Barrier (pp. 385-400)
    Pardridge, William M.
27. Blood???Brain Barrier Genomics (pp. 401-418)
    Boado, Ruben J.
28. Dynamic In Vitro Model of the Blood???Brain Barrier: Gene Profiling Using cDNA Microarray Analysis (pp. 419-434)
    Marroni, Matteo; Kight, Kelly M.; Hossain, Mohammed; Cucullo, Luca; Desai, Shailesh Y.; Janigro, Damir
29. Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells (pp. 435-450)
    Tanha, Jamshid; Muruganandam, Arumugam; Stanimirovic, Danica
30. mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization (pp. 451-464)
    Zagzag, David; Chan, Wai
31. Proteomics of Brain Endothelium: Separation of Proteins by Two-Dimensional Gel Electrophoresis and Identification by Mass Spectrometry (pp. 465-478)
    Haseloff, Reiner F.; Krause, Eberhard; Blasig, Ingolf E.
32. Immunoblot Detection of Brain Vascular Proteins (pp. 479-488)
    Matson, Christian T.; Drewes, Lester R.
33. Immunohistochemical Detection of Endothelial Proteins (pp. 489-502)
    Nag, Sukriti
34. Cerebral Vascular Function in Genetically Altered Mice (pp. 505-512)
    Faraci, Frank M.
35. Generation of Transgenic Mice by Pronuclear Injection (pp. 513-528)
    Damert, Annette; Kusserow, Heike
36. Mouse Genome Modification (pp. 529-544)
    Rozmahel, Richard

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