Library Science

This collection of self-contained, ??°hands-on??² descriptions of established laboratory protocols concentrates on receptor-mediated cell signaling, with particular attention devoted to those receptors that are part of the G-protein-linked superfamily. It offers those already working in the field as well as those in related areas the opportunity to expand their range of experimental techniques and to carry out successful investigations without needing to refer to any other methodological source.

Authors: Kendall, David A.; Hill, Stephen J.

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Table of Contents

1. The Problems and Pitfalls of Radioligand Binding (pp. 1-16)
   Keen, Mary
2. Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor (pp. 17-24)
   Henley, Jeremy M.; Kirkham, David M.
3. Autoradiography of ?÷1- and ?÷2-Adrenoceptors (pp. 25-40)
   Summers, Roger J.; Molenaar, Peter
4. In Situ Hybridization (pp. 41-50)
   Morris, Brian J.
5. Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes (pp. 51-62)
   Sweeney, Marva
6. Measurement of Adenylyl Cyclase Activity in Cell Membranes (pp. 63-78)
   Williams, Robert J.; Kelly, Eamonn
7. The Measurement of Cyclic AMP Levels in Biological Preparations (pp. 79-90)
   Alexander, Stephen P. H.
8. Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay (pp. 91-106)
   Horton, Jeffrey K.; Baxendale, Peter M.
9. Measurement of Cyclic GMP Formation (pp. 107-112)
   Hernandez, Felix
10. Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay (pp. 113-122)
    Murray, Kenneth J.
11. Purification and Activation of Cyclic GMP-Dependent Protein Kinase (pp. 123-128)
    Kuno, Takayoshi; Mukai, Hideyuki
12. The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity (pp. 129-150)
    Shahid, Mohammed; Nicholson, C. David
13. Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC) (pp. 151-166)
    Jenkinson, Stephen
14. Mass Measurement of Key Phosphoinositide Cycle Intermediates (pp. 167-176)
    Challiss, R.A. John
15. Measurement of Phospholipase C Activity in Brain Membranes (pp. 177-188)
    Claro, Enrique; Sarri, Elisabet; Picatoste, Fernando
16. Measurement of Diacylglycerol: Origins of Receptor-Stimulated Diacylglycerol Accumulation (pp. 189-202)
    Boarder, Michael R.; Purkiss, John R.
17. Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2 (pp. 203-214)
    Iredale, Philip A.; Dickenson, John M.
18. Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3 (pp. 215-228)
    Wilcox, Robert A.; Strupish, James; Nahorski, Stefan R.
19. Single-Cell Calcium Imaging (pp. 229-238)
    Marsh, Katrina A.
20. Characterization of Calcium/Calmodulin-Stimulated Protein Kinase II (pp. 239-260)
    Jarvie, Paula E.; Dunkley, Peter R.
21. Protein Kinase C: Measurement of Translocation, Activation, and Role in Cellular Responses (pp. 261-270)
    Wilkinson, Sandra E.; Hallam, Trevor J.
22. The Measurement of Phospholipase D-Linked Signaling in Cells (pp. 271-278)
    Wakelam, Michael J. O.; Hodgkin, Matthew; Martin, Ashley
23. Phospholipase A2 Activity (pp. 279-284)
    Burch, Ronald M.
24. Techniques for the Measurement of Nitric Oxide (pp. 285-300)
    Leone, Anna M.; Rhodes, Peter; Furst, Vanessa; Moncada, Salvador

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