Renal Cancer: Methods and Protocols
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Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological techniques that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.
Authors: Mydlo, Jack H.
Table of Contents
- Renal Cell Carcinoma: Clinical Features and Management (pp. 003-033)
Russo, Paul - Pathology of Kidney Tumors (pp. 035-051)
Grignon, David J - Telomerase Assay in Renal Cancer (pp. 055-067)
Zhang, William W; Klotz, Laurence H - Comparative Genomic Hybridization (pp. 069-080)
Presti, Joseph C - Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry (pp. 081-093)
Baffa, Raffaele; Calviello, Coleen M; Druck, Teresa; Gomella, Leonard G - Detection of Apoptosis in Renal Cell Carcinoma (pp. 095-100)
Pienta, Kenneth J; Williams, Jeffrey F - Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes (pp. 101-123)
Macoska, Jill A - Polymerase Chain Reaction Detection of DNA Sequence Deletions (pp. 125-137)
Macoska, Jill A - Matrix Metalloproteinases and Their Inhibitors (pp. 139-150)
Kugler, Alexander; Thelen, Paul; Ringert, Rolf-Hermann - Purification of Growth Factor mRNA in Renal Tissues: bFGF-2, FGF-2, TGFa , and EGFR (pp. 151-163)
Mydlo, Jack H - Laser-Capture Microdissection (pp. 165-173)
Griewe, Greg L; Dean, Robert; Zhang, Wei; Sesterhenn, Isabella A; Srivastava, Shiv; Moul, Judd W - Expression of c -met and WT-1 (pp. 175-191)
Kushner, Leslie; Chiu, Pui Yan; Pinto, Peter; Weiss, Gary Hal - Molecular Analysis of the von Hippel-Lindau Disease Gene (pp. 193-216)
Chernoff, Allen; Kasparcova, Viera; Linehan, W Marston; Stolle, Catherine A - Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma (pp. 217-242)
Vessella, Robert L; Wilbur, D Scott; Buhler, Kent; Lange, Paul H - Orthotopic Model of Renal Cell Carcinoma (pp. 245-253)
Nanus, David M; Engelstein, Dov - Murine Animal Model (pp. 255-264)
Nishisaka, Nobuyasu; Morse, Philo; Jones, Richard F; Wang, Ching Y; Haas, Gabriel P - Angiogenesis Assays (pp. 265-275)
Mydlo, Jack H - Antiangiogenic Approaches to Renal Cell Carcinoma: Assays for Inhibitors and Clinical Trials (pp. 277-298)
Campbell, Steven C; Reiher, Frank K; Smith, Norman D - Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma (pp. 301-312)
Patel, Belur; Belldegrun, Arie - Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination (pp. 313-321)
Browne, Wilbur B; Hawkins, William G; Lewis, Jonathan J - Monoclonal Antibodies: Generation and Clinical Use (pp. 323-341)
Oosterwijk, Egbert - Treatment Strategies in Metastatic Renal Cell Carcinoma: Cytokines, Vaccines, and Gene Therapy (pp. 343-359)
Mulders, Peter F - Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma (pp. 361-376)
Uzzo, Robert G; Kolenko, Vladimir; Novick, Andrew C; Finke, James H - Transforming Growth Factor-?1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma (pp. 377-384)
Adler, Howard L - Static and Flow Cytometry (pp. 385-399)
Madeb, Ralph; Pode, Dov; Nativ, Ofer