Library Science

In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschoff and J?rgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of novel anticancer therapeutics based on recombinant antibody technology. These readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.

Download Book

Table of Contents

1. Generation of Antibody Molecules Through Antibody Engineering (pp. 03-26)
   Kipriyanov, Sergey M.
2. Application of Recombinant Antibodies in Cancer Patients (pp. 27-54)
   Krauss, Jurgen; Arndt, Michaela; Pfreundschuh, Michael
3. DNA Immunization as a Means to Generate Antibodies to Proteins (pp. 57-62)
   Chowdhury, Partha S.
4. Chimerization of a Monoclonal Antibody for Treating Hodgkin’s Lymphoma (pp. 63-80)
   Krauss, Jurgen; Forster, Hans Heinrich; Uchanska-Ziegler, Barbara; Ziegler, Andreas
5. Humanization of Monoclonal Antibodies by CDR Grafting (pp. 81-100)
   O’Brien, Siobhan; Jones, Tarran
6. Generation and Screening of a Modular Human scFv Expression Library from Multiple Donors (pp. 103-122)
   Welschof, Martin; Christ, Christiane; Hermes, Ingrid; Keller, Armin; Kleist, Christian; Braunagel, Michael
7. Construction of Semisynthetic Antibody Libraries (pp. 123-132)
   Braunagel, Michael
8. Single-Domain VH Antibody Fragments from a Phage Display Library (pp. 133-144)
   Binyamin, Liat; Plaksin, Daniel; Reiter, Yoram
9. Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection (pp. 145-160)
   Figini, mariangela; Green, Andrew; Colotta, Francesco; Canevari, Silvana
10. Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Display Libraries (pp. 161-178)
    Engberg, Jan; Yenidunya, Ali F.; Clausen, Rikke; Jensen, Liselotte B.; Sorensen, Peter; Kops, Pernille; Riise, Erik
11. Engineering Hot Spots for Affinity Enhancement of Antibodies (pp. 179-196)
    Chowdhury, Partha S.
12. Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies (pp. 197-212)
    Wu, Herren
13. Tailoring Kinetics of Antibodies Using Focused Combinatorial Libraries (pp. 213-234)
    Wu, Herren; An, Ling-Ling
14. Engineering scFvs for Improved Stability (pp. 237-254)
    Chowdhury, Partha S.; Vasmatzis, George
15. Recombinant Single-Chain and Disulfide-Stabilized Fv Immunotoxins for Cancer Therapy (pp. 255-268)
    Niv, Revital; Segal, Dina; Reiter, Yoram
16. Generation of Recombinant Immunotoxins for Specific Targeting of Tumor-Related Peptides Presented by MHC Molecules (pp. 269-282)
    Cohen, Cyril J.; Denkberg, Galit; Segal, Dina; Reiter, Yoram
17. Construction and Characterization of RNase-Based Targeted Therapeutics (pp. 283-304)
    Newton, Dianne L.; Futami, Junichiro; Ruby, Dale; Rybak, Susanna M.
18. Bispecific Diabodies for Cancer Therapy (pp. 305-322)
    Arndt, Michaela; Krauss, Jurgen
19. Generation and Characterization of Bispecific Tandem Diabodies for Tumor Therapy (pp. 323-334)
    Kipriyanov, Sergey M.
20. Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy (pp. 335-350)
    Power, Barbara E.; Kortt, Alexander A.; Hudson, Peter J.
21. Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas (pp. 351-364)
    Yazaki, Paul J.; Wu, Anna M.
22. Generation, Expression, and Monitoring of Recombinant Immune Receptors for Use in Cellular Immunotherapy (pp. 365-382)
    Hombach, Andreas; Heuser, Claudia; Abken, Hinrich
23. Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization (pp. 383-392)
    Brekke, Ole H.; Thommesen, John E.
24. Single-Chain Fv-Based Affinity Purification of the Cellular Stress Protein gp96 for Vaccine Development (pp. 393-420)
    Kleist, Christian; Arnold-Schild, Daniele; Welschof, Martin; Finger, Martina; Opelz, Gerhard; Rammensee, Hans-Georg; Schild, Hansjorg; Terness, Peter
25. Recombinant Adenoviruses for In Vivo Expression of Antibody Fragments (pp. 421-434)
    Kontermann, Roland E.; Korn, Tina; Jerome, Valerie
26. Production of Antibody Fragments in a Bioreactor (pp. 437-442)
    Hellebust, Halldis
27. Large Scale Production of Recombinant Antibodies by Utilizing Cellulose-Binding Domains (pp. 443-454)
    Benhar, Itai; Berdichevsky, Yevgeny
28. Production of Tumor-Specific Antibodies in Tobacco (pp. 455-466)
    Vaquero-Martin, Carmen; Fischer, Rainer

This entry was posted on Saturday, April 28th, 2007 at 4:14 pm and is filed under Molecular Biology. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.