Quantitative PCR Protocols
posted in Library |
This is a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. With its step-by-step instructions, Quantitative PCR Protocols allows researchers to address biological and diagnostic questions that are difficult or impossible to answer using any other experimental approach. Ed. B.Kochanowski, U.Reischl
Table of Contents
- Part I. Reviews
Quantitative PCR: A Survey of the Present Technology
Udo Reischl and Berndt Kochanowski
General Principles of Quantitative PCR
Luc Raeymaekers
Effects of Collection, Processing, and Storage on RNA Detection and Quantification
Mark Holodniy
Quantitative RT-PCR
Paul D. Siebert
Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Olivier Lantz, Elizabeth Bonney, Franck Griscelli, and Yassine Taoufik
Comparison of Competitive PCR and Positive Control-Based PCR
Francois Mallet
Part II. ProtocolsEnd-Point Titration-PCR for Quantitation of Cytomegalovirus DNA
Jerzy K. Kulski
Analysis of Amplified DNA Molecules by Capillary Electrophoresis and Laser Induced Fluorescence
Michael J. Fasco
Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquid Chromatography (HPLC)
Thomas Kohler
Quantifying Amplicons with ELISA
Olivier Lantz, Elizabeth Bonney, and Yassine Taoufik
Competitive PCR Quantitation Utilizing a Microtiter Plate Based Format for the Detection of PCR Products
Bernd Kochanowski and Wolfgang Jilg
Competitive and Differential RT-PCR (CD-RT-PCR) for Measurement of Normalized Gene Expression Using Antisense Competitors
Eric de Kant
Amplified Assay for Specific Dual-Labeled DNA Using the Coagulation Cascade (EDNA-ELCA)
George J. Doellgast, G. Alan Beard, Margaret Sheehan, Nathan Iyer, Louis S. Kucera, and Stephen H. Richardson
Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Suppressor Gene
Meinhard Hahn and Alfred Pingoud
Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing
Anu Suomalainen and Ann-Christine Syvanen
High Resolution PCR Quantitation by AmpliSensor Assay
Chang-Ning Wang
Construction of Polycompetitors for Competitive PCR
David B. Corry and Richard M. Locksley
Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA
Marlyse C. Knuchel and Aftab A. Ansari
A Stochastic PCR Approach for RNA Quantification in Multiple Samples
Adrian Puntschart and Michael Vogt
Quantitation of mRNA Species by RT-PCR on Total mRNA Population with Nonradioactive Probes
Sabine Herblot, Benoit Rousseau, and Jacques Bonnet