Library Science

Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.

Authors: Hyde, John E.

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Table of Contents

1. Culturing and Biological Cloning of Trypanosoma brucei (pp. 1-14)
   Carrington, Mark
2. Culturing and Biological Cloning of Trypanosoma cruzi (pp. 15-28)
   Miles, Michael A.
3. In Vitro Cultivation and Biological Cloning of Leishmania (pp. 29-42)
   Evans, David A.
4. Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages) Suitable for Large-Scale Preparations (pp. 43-56)
   Read, Martin; Hyde, John E.
5. Synchronization and Cloning of Malaria Parasites (pp. 57-66)
   Walliker, David; Beale, Geoffrey
6. The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies (pp. 67-88)
   Carter, Richard; Ranford-Cartwright, Lisa; Alano, Pietro
7. The Culture of Schistosoma mansoni and Production of Life Cycle Stages (pp. 89-100)
   Hackett, Fiona
8. Preparation of DNA and RNA from llypanosoma brucei (pp. 101-112)
   Cartington, Mark
9. Isolation of RNA and DNA from llypanosoma cruzi (pp. 113-122)
   Ashall, Frank
10. Isolation of DNA and RNA from Leishmania (pp. 123-132)
    Kelly, John M.
11. The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum (pp. 133-144)
    Hyde, John E.; Read, Martin
12. Extraction and Purification of Nucleic Acids from Schistosomes (pp. 145-154)
    Jefi, Simon A.; Simpson, Andrew J.
13. Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting (pp. 155-168)
    Hughes, Margaret A.; Crampton, Julian M.
14. The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification (pp. 169-190)
    Hughes, Margaret A.; Crampton, Julian M.
15. Analysis of the DNA of Parasitic Protozoa by Flow Cytometry (pp. 191-204)
    Dvorak, James A.
16. PCR For Low-Level Detection of Malaria Parasites in Blood (pp. 205-212)
    Tirasophon, Witoon; Panyim, Sakol
17. PCR Methods for Identification of Point Mutations and Gene Rearrangements (pp. 213-222)
    Peterson, David S.
18. Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques (pp. 223-248)
    Marshall, Vikki M.; Coppel, Ross L.
19. Ribosomal RNA Probes for Detection and Identification of Species (pp. 249-264)
    Bachellerie, Jean-Pierre; Qu, Liang-Hu
20. Rapid Sequencing of Parasite rRNA (pp. 265-276)
    Bachellerie, Jean-Pierre; Qu, Gang-Hu
21. Antibody Screening of Expression Libraries (pp. 277-296)
    Coppel, Ross L.; Smith, Fiona M.; Petersen, Carolyn
22. Antibody Select Procedure for Characterization of Expression Clones (pp. 297-302)
    Ridley, Robert G.; Hall, Roger
23. Isolation of Parasite Genes Using Synthetic Oligonucleotides (pp. 303-318)
    Hyde, John E.; Holloway, Stephen P.
24. Chromosome Mapping Methods for Parasitic Protozoa (pp. 319-332)
    Dolan, Stephen A.; Adam, Rodney D.; Wellems, Thomas E.
25. Transfection of Leishmania and Trypanosoma brucei by Electroporation (pp. 333-348)
    Beverley, Stephen M.; Clayton, Christine E.
26. Two-Dimensional Polyacrylamide Gel Electrophoresis (pp. 349-360)
    Fenton, Brian
27. Isoenzyme Electrophoresis for Parasite Characterization (pp. 361-382)
    Abderrazak, Souha Ben; Guerrini, Francoise; Mathieu-Daude, Francoise; Truc, Philippe; Neubauer, Katja; Lewicka, Katarzyna; Barnabe, Christian; Tibayrenc, Michel
28. The Surface Labeling of Schistosomes (pp. 383-388)
    Simpson, Andrew J. G.
29. Monoclonal Antibody Affinity Chromatography (pp. 389-396)
    Hunt, Roger Hall Philip D.; Ridley, Robert G.
30. Immunoblotting and Enzyme-Linked Immunosorbent Assay (pp. 397-406)
    Hunt, Philip D.; Hall, Roger
31. Immunofluorescence of Parasites (pp. 407-414)
    Sherwin, Pevor; Read, Martin
32. Immunoelectron Microscopic Localization of Antigens in Malaria Parasites (pp. 415-430)
    Bannister, Lawrence H.; Kent, Andrew P.
33. Preparation of Blotted Membrane for Protein Microsequencing (pp. 431-440)
    Sirawaraporn, Worachart
34. Identification of T-Cell Epitopes (pp. 441-458)
    Suss, Gabriele; Pink, J. Richard L.; Meloen, Rob; Takacs, Bela; Sinigaglia, Francesco

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