Library Science

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

Authors: Agrawal, Sudhir

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Table of Contents

1. Protecting Groups in Oligonucleotide Synthesis (pp. 1-72)
   Sonveaux, Etienne
2. Incorporation of Modified Bases into Oligonucleotides (pp. 73-92)
   Meyer, Rich B.
3. Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups (pp. 93-120)
   Agrawal, Sudhir
4. Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups (pp. 121-144)
   Fidanza, Jacqueline A.; Ozaki, Hiroaki; McLaughlin, Larry W.
5. Postsynthesis Functionalization of Oligonucleotides (pp. 145-166)
   Chu, Barbara C. E; Orgel, Leslie E.
6. Oligonucleotide-Enzyme Conjugates (pp. 167-186)
   Ruth, Jerry L.
7. Oligonucleotides Containing Degenerate Bases: Synthesis and Uses (pp. 187-206)
   Lin, Paul Kong Thoo; Brown, Daniel M.
8. Synthesis of [15N]-Labeled DNA Fragments (pp. 207-232)
   Jones, Roger A.
9. Analysis and Purification of Synthetic Oligonucleotides by High-Performance Liquid Chromatography (pp. 233-264)
   Warren, William J.; Vella, George
10. Sequence Analysis of Oligodeoxyribonucleotides (pp. 265-276)
    Black, Diane M.; T., Peter
11. Gel-Capillary Electrophoresis Analysis of Oligonucleotides (pp. 277-300)
    Andrus, Alex
12. Nuclear Magnetic Resonance Studies of Oligonucleotides (pp. 301-318)
    Jamszewski, Jemy W.; Roy, Siddhartha; Cohen, Jack S.
13. Mass Spectrometry of Nucleotides and Oligonucleotides (pp. 319-346)
    McClure, Thomas D.; Schram, Karl H.
14. Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions (pp. 347-372)
    Breslauer, Kenneth J.

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