Library Science

Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides for both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.

protein-sequencing-protocols-2nd-edition.pdf

Table of Contents

1. Strategies for Handling Polypeptides on a Micro-Scale (pp. 01-18)
   Smith, Bryan John; Tempst, Paul
2. SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing (pp. 19-30)
   Smith, Bryan John
3. Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization (pp. 31-40)
   Dunn, Michael J.
4. Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization (pp. 41-48)
   Dunn, Michael J.
5. Enzymatic Cleavage of Proteins (pp. 49-62)
   Smith, Bryan John
6. Chemical Cleavage of Polypeptides (pp. 63-82)
   Smith, Bryan John
7. Enzymatic Preparation and Isolation of Glycopeptides (pp. 83-90)
   Harvey, David J.
8. Analytical and Micropreparative Capillary Electrophoresis of Peptides (pp. 91-100)
   Smith, Alan J.
9. High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues* (pp. 101-110)
   Turck, Christoph W.
10. Hydrolysis of Samples for Amino Acid Analysis (pp. 111-122)
    Davidson, Ian
11. Amino Acid Analysis: Precolumn Derivatization Methods (pp. 123-132)
    Irvine, G. Brent; Davidson, Ian
12. Post Column Amino Acid Analysis (pp. 133-142)
    Smith, Alan J.
13. Amino Acid Analysis Using Pre-Column Derivatization with 6-Aminoquinolyl- NHydroxysuccinimidyl Carbamate: Analysis of Hydrolyzed Proteins and Electroblotted Samples (pp. 143-154)
    Cohen, Steven A.
14. Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection (pp. 155-168)
    Jandik, Petr; Cheng, Jun; Avdalovic, Nebojsa
15. D -Amino Acid Analysis (pp. 169-180)
    Scaloni, Andrea; Simmaco, Maurizio; Bossa, Francesco
16. Validation of Amino Acid Analysis Methods (pp. 181-194)
    Reason, Andrew J.
17. Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins (pp. 195-210)
    Greer, Fiona M.; Morris, Howard R.
18. Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry (pp. 211-220)
    Pappin, Darryyl J. C.
19. Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes (pp. 221-234)
    Shevchenko, Anna; Sunyaev, Shamil; Liska, Adam; Bork, Peer; Shevchenko, Andrej
20. Direct Identification of Proteins in Ultracomplex Mixtures: Applications to Proteome Analysis (pp. 235-246)
    Schieltz, David M.; Yates, John R.
21. Identification of PTH-Amino Acids by HPLC (pp. 247-268)
    Grant, Gregory A.; Crankshaw, Mark W.
22. Protein Sequencer Maintenance and Troubleshooting (pp. 269-286)
    Dunbar, Bryan
23. N-Terminal Protein Sequencing for Special Applications (pp. 287-300)
    Jackson, Philip J.
24. Identification of Phosphorylation Sites by Edman Degradation (pp. 301-308)
    Steinke, Laurey; Cook, Richard G.
25. Validation of Protein Sequencing in a Regulated Laboratory (pp. 309-318)
    Jackson, Philip J.; Dodsworth, Neil
26. Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method (pp. 319-332)
    Dupont, David R.; Yuen, Sylvia W.; Graham, Kenneth S.
27. Chemical Modifications of Proteins as an Aid to Sequence Analysis (pp. 333-354)
    Carne, Alex F.
28. Deblocking of N-Terminally Modified Proteins (pp. 355-364)
    Hirano, Hisashi; Kamp, Roza M.
29. Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid (pp. 365-370)
    Mozdzanowski, Jacek
30. Identification of Sites of Glycosylation (pp. 371-384)
    Harvey, David J.
31. Analysis of Sites of Protein Phosphorylation (pp. 385-398)
    Aitken, Alastair; Learmonth, Michele
32. Quantitation and Location of Disulfide Bonds in Proteins (pp. 399-410)
    Aitken, Alastair; Learmonth, Michele
33. Getting the Most from Your Protein Sequence (pp. 411-430)
    Copley, Richard R.; Russell, Robert B.

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