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This new edition of Protein Purification Protocols (1996) completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.

Ed. Cutler, Paul

protein-purification-protocols-2nd-edprotein-purification-protocols-2nd-ed.pdf

Table of Contents

1. General Strategies (pp. 1-14)
   Doonan, Shawn; Cutler, Paul
2. Preparation of Extracts From Animal Tissues (pp. 15-20)
   Skehel, J. Mark
3. Protein Extraction From Plant Tissues (pp. 21-28)
   Fido, Roger J.; Mills, E. N. Clare; Rigby, Neil M.; Shewry, Peter R.
4. Extraction of Recombinant Protein From Bacteria (pp. 29-36)
   McGettrick, Anne. F.; Worrall, D. Margaret
5. Protein Extraction From Fungi (pp. 37-46)
   Bridge, Paul D.; Kokubun, Tetsuo; Simmonds, Monique S. J.
6. Subecllular Fractionation of Animal Tissues (pp. 47-52)
   Ryan, Norma M.
7. Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria (pp. 53-64)
   Tobin, Alyson K.; Bowsher, Caroline G.
8. The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds (pp. 65-74)
   Pierpoint, William S.
9. Avoidance of Proteolysis in Extracts (pp. 75-84)
   Beynon, Robert J.; Oliver, Simon
10. Concentration of Extracts (pp. 85-90)
    Doonan, Shawn
11. Making and Changing Buffers (pp. 91-100)
    Doonan, Shawn
12. Purification and Concentration by Ultrafiltration (pp. 101-116)
    Schratter, Paul
13. Bulk Purification by Fractional Precipitation (pp. 117-124)
    Doonan, Shawn
14. Ion-Exchange Chromatography (pp. 125-132)
    Selkirk, Chris
15. Hydrophobic Interaction Chromatography (pp. 133-138)
    O’Farrell, Paul A.
16. Affinity Chromatography (pp. 139-150)
    Cutler, Paul
17. Dye-Ligand Affinity Chromatography (pp. 151-158)
    McGettrick, Anne F.; Worrall, D. Margaret
18. Lectin Affinity Chromatography (pp. 159-166)
    West, Iris; Goldring, Owen
19. Immunoaffinity Chromatography (pp. 167-178)
    Cutler, Paul
20. Immobilized Metal-Ion Affinity Chromatography (pp. 179-190)
    Yip, Tai-Tung; Hutchens, T. William
21. Chromatography on Hydroxyapatite (pp. 191-194)
    Doonan, Shawn
22. Thiophilic Affinity Chromatography and Related Methods (pp. 195-204)
    Matejtschuk, Paul
23. Affinity Precipitation Methods (pp. 205-224)
    Irwin, Jane A.; Tipton, Keith F.
24. Isoelectric Focusing (pp. 225-232)
    Westermeier, Reiner
25. Chromatofocusing (pp. 233-238)
    Mantle, Timothy J.; Noone, Patricia
26. Size-Exclusion Chromatography (pp. 239-252)
    Cutler, Paul
27. Fast Protein Liquid Chromatography (pp. 253-258)
    Sheehan, David; O’Sullivan, Siobhan
28. Reversed-Phase Chromatography of Proteins (pp. 259-282)
    Neville, William A.
29. Extraction of Membrane Proteins (pp. 283-294)
    Ohlendieck, Kay
30. Removal of Detergent From Protein Fractions (pp. 295-300)
    Ohlendieck, Kay
31. Purification of Membrane Proteins (pp. 301-308)
    Ohlendieck, Kay
32. Lyophilization of Proteins (pp. 309-322)
    O’Fagain, Ciaran
33. Storage of Pure Proteins (pp. 323-338)
    O’Fagain, Ciaran
34. Electroelution of Proteins From Polyacrylamide Gels (pp. 339-344)
    Dunn, Michael J.
35. Electroblotting of Proteins From Polyacrylamide Gels (pp. 345-352)
    Dunn, Michael J.
36. Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses (pp. 353-360)
    Jones, Neil A.
37. Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis (pp. 361-376)
    Zuo, Xun; Speicher, David W.
38. Practical Column Chromatography (pp. 377-388)
    Doonan, Shawn
39. Detection Methods (pp. 389-410)
    Mozdzanowski, Jacek; Burman, Sudhir
40. Peptide Proteomics (pp. 411-424)
    McNulty, Dean E.; Slemmon, J. Randall
41. Multidimensional Liquid Chromatography of Proteins (pp. 425-446)
    Watson, Rod; Nadler, Tim
42. Mass Spectrometry (pp. 447-454)
    Bell, David J.
43. Purification of Therapeutic Proteins (pp. 455-462)
    Bonnerjea, Julian
44. Purification Process Scale-Up (pp. 463-480)
    Prince, Karl; Smith, Martin

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