11th
April
2007
Protein Kinase C Protocols
posted in Library, Molecular Biology |
The first compilation of classic and emerging laboratory techniques for the study of the biochemistry and cell biology of protein kinase C (PKC). Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C.. Each protocol is introduced in the context of PKC function and regulation and contains many notes on how best to deal with the problems that may occur. Comprehensive and authoritative, Protein Kinase C Protocols is a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that brings protein kinase C research into any laboratory interested in studying it.
Ed. Newton, Alexandra C. ISBN: 1588290689
Table of Contents
- The Ins and Outs of Protein Kinase C (pp. 3-8)
Newton, Alexandra C. - Early Studies of Protein Kinase C: A Historical Perspective (pp. 9-18)
Nishizuka, Yasutomi; Kikkawa, Ushio - Expression and Purifi cation of Protein Kinase C from Insect Cells (pp. 21-34)
Mukai, Hideyuki; Ono, Yoshitaka - Expression and Purifi cation of Protein Kinase Cð from Bacteria (pp. 35-42)
Gschwendt, Michael; Stempka, Luise - Complexities in Protein Kinase C Activity Assays: An Introduction (pp. 45-62)
Sando, Julianne J. - Enzyme Assays for Protein Kinase C Activity (pp. 63-76)
Sando, Julianne J.; Beals, Jacquelyn K. - Subcellular Translocation of Protein Kinase C (pp. 77-86)
Sando, Julianne J.; Beals, Jacquelyn K.; Hussaini, Isa M. - Measuring the Interaction of Protein Kinase C with Membranes: An Introduction (pp. 89-92)
Newton, Alexandra C. - Fluorescence Imaging of Protein Kinase C Translocation in Living Cells (pp. 93-104)
Saito, Naoaki - Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles (pp. 105-114)
Giorgione, Jennifer; Newton, Alexandra C. - Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C (pp. 115-128)
Nalefski, Eric A.; Newton, Alexandra C. - [3H]Phorbol 12,13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins (pp. 129-156)
Lewin, Nancy E.; Blumberg, Peter M. - Protein Kinase C Phosphorylation: An Introduction (pp. 159-162)
Parker, Peter J. - Pulse-Chase Analysis of Protein Kinase C (pp. 163-170)
Takahashi, Mikiko; Ono, Yoshitaka - PDK-1 and Protein Kinase C Phosphorylation (pp. 171-190)
Toker, Alex - In Vitro Autophosphorylation of Protein Kinase C Isozymes (pp. 191-206)
Pepio, Antonio M.; Sossin, Wayne S. - Tyrosine Phosphorylation of Protein Kinase C (pp. 207-216)
Yamamoto, Toshiyoshi; Yamauchi, Emiko; Taniguchi, Hisaaki; Matsuzaki, Hidenori; Kikkawa, Ushio - Methods to Study Dephosphorylation of Protein Kinase C In Vivo (pp. 217-232)
England, Karen; Rumsby, Martin - Phosphopeptide-Specifi c Antibodies to Protein Kinase C (pp. 233-244)
Sossin, Wayne S. - Identifying Protein Kinase C Substrates: An Introduction (pp. 247-252)
Toker, Alex - A Chemical Genetic Approach for the Identifi cation of Direct Substrates of Protein Kinases (pp. 253-272)
Shah, Kavita; Shokat, Kevan M. - Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries (pp. 273-286)
Yaffe, Michael B. - Structural Analysis of Protein Kinase C:An Introduction (pp. 289-290)
Hurley, James H. - Bacterial Expression and Purifi cation of C1 and C2 Domains of Protein Kinase C Isoforms (pp. 291-298)
Cho, Wonhwa; Digman, Michelle; Ananthanarayanan, Bharath; Stahelin, Robert V. - Crystallization of the Protein Kinase Cð C1B Domain (pp. 299-304)
Zhang, Gongyi; Hurley, James H. - Methods for Detecting Binding Proteins: An Introduction (pp. 307-326)
Endemann, Gerda; Mochly-Rosen, Daria - Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis (pp. 327-344)
Chang, Betty Y.; Cartwright, Christine A. - Glutathione S-Transferase Pull-Down Assay (pp. 345-350)
Schechtman, Deborah; Mochly-Rosen, Daria; Ron, Dorit - Overlay Method for Detecting Protein-Protein Interactions (pp. 351-358)
Schechtman, Deborah; Murriel, Christopher; Bright, Rachel; Mochly-Rosen, Daria - An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates (pp. 359-368)
Jaken, Susan - Functional Proteomic Analysis of the Protein Kinase C Signaling System (pp. 369-386)
Pass, Jason M.; Zhang, Jun; Vondriska, Thomas M.; Ping, Peipei - Pharmacological Probes for Protein Kinase C: An Introduction (pp. 389-396)
Kazanietz, Marcelo G. - Applications of Inhibitors for Protein Kinase C and Their Isoforms (pp. 397-422)
Shen, Garry X.; Way, Kerrie J.; Jacobs, Judith R. C.; King, George L. - Phorbol Esters as Probes for the Study of Protein Kinase C Function (pp. 423-440)
Kazanietz, Marcelo G.; Lorenzo, Patricia S. - Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs (pp. 441-450)
O’Brian, Catherine A.; Ward, Nancy E.; Chu, Feng; Stewart, Jubilee R. - Genetic Approaches to Studying Protein Kinase C: An Introduction (pp. 453-454)
Messing, Robert O. - Animal Models in the Study of Protein Kinase C Isozymes (pp. 455-474)
Choi, Doo-Sup; Messing, Robert O. - Genetic Manipulation of Protein Kinase C In Vivo (pp. 475-490)
Toker, Alex - Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms (pp. 491-516)
Parissenti, Amadeo M.; Riedel, Heimo - Protein Kinase C in Disease: Cancer (pp. 519-538)
Fields, Alan P.; Gustafson, W. Clay - Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models (pp. 539-554)
Fields, Alan P.; Murray, Nicole R.; Gustafson, W. Clay - Alcohol Addiction (pp. 555-570)
Olive, M. Foster; Ron, Dorit