Library Science

A diverse collection of unique methodologies to synthesize and construct protein arrays for basic and clinical research in a high-throughput manner. These readily reproducible techniques can be applied to such applications as quantifying specific proteins of interest and discovering novel proteins, as well as to a variety of affinity substances, including antibodies, peptides, proteins, aptamers, and chemicals. The techniques can be used for biomarkers discovery, assay development, clinical sample testing, and building the next generation of molecular tools.

Authors: Fung, Eric

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Table of Contents

1. Protein Arrays From cDNA Expression Libraries (pp. 001-014)
   Weiner, Hendrik; Faupel, Thomas; Bussow, Konrad
2. Protein Expression Arrays for Proteomics (pp. 015-024)
   Gilbert, Michele; Edwards, Todd C.; Albala, Joanna S.
3. Generation of Protein In Situ Arrays by DiscernArray™ Technology (pp. 025-032)
   He, Mingyue
4. Multiplexed Protein Analysis Using Spotted Antibody Microarrays (pp. 033-046)
   Haab, Brian B.; Zhou, Heping
5. Target-Assisted Iterative Screening of Phage Surface Display cDNA Libraries (pp. 047-060)
   Kurakin, Alexei; Wu, Susan; Bredesen, Dale E.
6. Design, Construction, and Use of Tissue Microarrays (pp. 061-072)
   Hewitt, Stephen M.
7. A Streptavidin–Biotin-Based Microarray Platform for Immunoassays (pp. 073-084)
   Pavlickova, Petra; Hug, Hubert
8. Site-Specific Immobilization of Biotinylated Proteins for Protein Microarray Analysis (pp. 085-100)
   Lue, Rina Y. P.; Chen, Grace Y. J.; Zhu, Qing; Lesaicherre, Marie-Laure; Yao, Shao Q.
9. Processing of Photoaptamer Microarrays (pp. 101-110)
   Petach, Helen; Ostroff, Rachel; Greef, Chad; Husar, Gregory M.
10. Use of a Small Molecule-Based Affinity System for the Preparation of Protein Microarrays (pp. 111-122)
    Hughes, Karin A.
11. Evaluation of Antibodies and Microarray Coatings As a Prerequisite for the Generation of Optimized Antibody Microarrays (pp. 123-134)
    Angenendt, Philipp; Glokler, Jorn
12. Printing Functional Protein Microarrays Using Piezoelectric Capillaries (pp. 135-144)
    Delehanty, James B.
13. The Use of Precision Glass Syringes and a Noncontact Microsolenoid Dispenser for the Production of High-Throughput Low-Density Arrays (pp. 145-160)
    Azarani, Arezou
14. A Protein Microarray ELISA for Screening Biological Fluids (pp. 161-172)
    Varnum, Susan M.; Woodbury, Ronald L.; Zangar, Richard C.
15. Protein-Domain Microarrays (pp. 173-182)
    Espejo, Alexsandra; Bedford, Mark T.
16. SH3 Domain Protein-Binding Arrays (pp. 183-190)
    Chamnongpol, Sangpen; Li, Xianqiang
17. Site-Specific Peptide Immobilization Strategies for the Rapid Detection of Kinase Activity on Microarrays (pp. 191-204)
    Uttamchandani, Mahesh; Chen, Grace Y. J.; Lesaicherre, Marie-Laure; Yao, Shao Q.
18. Simultaneous Monitoring of Multiple Kinase Activities by SELDI-TOF Mass Spectrometry (pp. 205-214)
    Thulasiraman, Vanitha; Wang, Zheng; Katrekar, Anjali; Lomas, Lee; Yip, Tai-Tung
19. Cytokine Protein Arrays (pp. 215-232)
    Huang, Ruo-Pan
20. Fabrication and Application of G Protein-Coupled Receptor Microarrays (pp. 233-244)
    Fang, Ye; Webb, Brian; Hong, Yulong; Ferrie, Ann; Lai, Fang; Frutos, Anthony G.; Lahiri, Joydeep
21. ProteinChip® Array-Based Amyloid ß Assays (pp. 245-258)
    Bradbury, Lisa E.; LeBlanc, James F.; McCarthy, Diane B.
22. Serum Protein-Expression Profiling Using the ProteinChip® Biomarker System (pp. 259-270)
    Gilbert, Kate; Figueredo, Sharel; Meng, Xiao-Ying; Yip, Christine; Fung, Eric T.
23. Protein Arrays for Serodiagnosis of Disease (pp. 271-284)
    Bacarese-Hamilton, Tito; Ardizzoni, Andrea; Gray, Julian; Crisanti, Andrea

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