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Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

protein-and-peptide-analysis-by-mass-spectrometry.pdf

Table of Contents

1. Mass Spectrometry in the Analysis of Peptides and Proteins, Past and Present (pp. 1-8)
   Roepstorff, Peter
2. Mass Spectrometry: Ionization Methods and Instrumentation (pp. 9-28)
   Chapman, John R.
3. Charged Derivatives for Peptide Sequencing Using a Magnetic Sector Instrument (pp. 29-42)
   Zaia, Joseph
4. New Instrumental Approaches to Collision-Induced Dissociation Using a Time-of-Flight Instrument (pp. 43-56)
   Spengler, Bernhard
5. Mass Spectrometric Quantification of Neuropeptides (pp. 57-66)
   Desiderio, Dominic M.
6. The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting (pp. 67-82)
   Cottrell, John S.; Sutton, Chris W.
7. Liquid Chromatography/Mass Spectrometry for the Analysis of Protein Digests (pp. 83-100)
   Covey, Thomas
8. Structural Analysis of Protein Variants (pp. 101-114)
   Wada, Yoshinao
9. Noncovalent Interactions Observed Using Electrospray Ionization (pp. 115-128)
   Schwartz, Brenda L.; Gale, David C.; Smith, Richard D.
10. Protein Secondary Structure Investigated by Electrospray Ionization (pp. 129-140)
    Robinson, Carol V.
11. The Effect of Detergents on Proteins Analyzed by Electrospray Ionization (pp. 141-160)
    Loo, Rachele R. Ogorzalek; Dales, Natalie; Andrews, Philip C.
12. Posttranslational Modifications Analyzed by Automated Protein Ladder Sequencing (pp. 161-170)
    Wang, Rong; Chait, Brian T.
13. Rapid Analysis of Single-Cysteine Variants of Recombinant Proteins (pp. 171-184)
    Keough, Thomas W.; Sun, Yiping; Barnett, Bobby L.; Lacey, Martin P.; Bauer, Mark D.; Wang, Ellen S.; Erwin, Christopher R.
14. Disulfide Bond Location in Proteins (pp. 185-210)
    Sun, Yiping; Bauer, Mark D.; Keough, Thomas W.; Lacey, Martin P.
15. Determination of Loading Values and Distributions for Drugs Conjugated to Proteins and Antibodies by MALDI-MS and ESI-MS (pp. 211-226)
    Siegel, Marshall M.
16. Hydrophobic Proteins and Peptides Analyzed by Matrix-Assisted Laser Desorption/lonization (pp. 227-230)
    Schey, Kevin L.
17. Analysis of Glycoproteins and Glycopeptides Using Fast-Atom Bombardment (pp. 231-242)
    Rademaker, Geert Jan; Thomas-Oates, Jane
18. Identification of Cleaved Oligosaccharides by Matrix-Assisted Laser Desorptiotdonization (pp. 243-254)
    Harvey, David J.
19. Structural Characterization of Protein Glycosylation Using HPLC/ Electrospray Ionization Mass Spectrometry and Glycosidase Digestion (pp. 255-278)
    Settineri, Christine A.; Burlingame, Alma L.
20. Analysis of Complex Protein and Glycoprotein Mixtures by Electrospray Ionization Mass Spectrometry with Maximum Entropy Processing (pp. 279-294)
    Green, Brian N.; Hutton, Therese; Vinogradov, Serge N.
21. The Use of Databases in Searching the Literature of Biological Mass Spectrometry (pp. 295-300)
    Oliver, Ronald W. A.; Carrier, Michael P.

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