Library Science

A collection of basic and advanced molecular methods that reveal those markers essential for more accurate diagnoses of specific diseases, and in developing new treatment strategies. The techniques range from in vitro methods to in vivo models of prostate cancer, and include new methods for the accurate diagnosis of prostate cancer, proteome and microarray analyses, and new strategies for the treatment of refractory disease. Each method is described in step-by-step detail to ensure successful results and avoid failure.

Authors: Russell, Pamela J. ; Jackson, Paul ; Kingsley, Elizabeth A.

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Table of Contents

1. Epidemiological Investigation of Prostate Cancer (pp. 1-20)
   Giles, Graham G.
2. Human Prostate Cancer Cell Lines (pp. 21-40)
   Russell, Pamela J.; Kingsley, Elizabeth A.
3. Growth of Prostatic Epithelial and Stromal Cells In Vitro (pp. 41-58)
   Peehl, Donna M.
4. Prostate Epithelial Stem Cell Isolation and Culture (pp. 59-68)
   Hudson, David L.; Masters, John R.W.
5. Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer (pp. 69-78)
   Rhim, Johng S.
6. Spheroids of Prostate Tumor Cell Lines (pp. 79-88)
   Sgouros, George; Yang, Wei-Hong; Enmon, Richard
7. Animal Models of Prostate Cancer (pp. 89-112)
   Russell, Pamela J.; Voeks, Dale J.
8. Transgenic Mouse Models for Prostate Cancer: Identification of an Androgen-Dependent Promoter and Creation and Characterization of the Long Probasin Promoter-Large T Antigen (LPB-Tag) Model (pp. 113-148)
   Kasper, Susan; Tu, William; Roberts, Richard L.; Shappell, Scott B.
9. In Vivo Models of Human Prostate Cancer Bone Metastasis (pp. 149-162)
   Brown, Julie M.
10. Effects of Fixation on Tissues (pp. 163-180)
    Mortensen, Elin; Brown, Julie M.
11. Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma (pp. 181-198)
    Delprado, Warick
12. Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History (pp. 199-218)
    Gardiner, R. A.; Burger, Michelle; Clements, Judith A.; Lavin, Martin F.
13. Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples (pp. 219-240)
    Clark, Susan J.; Millar, Douglas S.; Molloy, Peter
14. Production and Characterization of Antipeptide Kallikrein 4 Antibodies: Use of Computer Modeling to Design Peptides Specific to Kallikrein 4 (pp. 241-254)
    Harvey, Tracey J.; Dong, Ying; Bui, Loan; Jarrott, Russell; Walsh, Terry; Clements, Judith A.
15. The Androgen Receptor CAG Repeat and Prostate Cancer Risk (pp. 255-266)
    Clark, Peter E.; Irvine, Ryan A.; Coetzee, Gerhard A.
16. Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer (pp. 267-276)
    Culig, Zoran; Hobisch, Alfred; Erdel, Martin; Bartsch, Georg; Klocker, Helmut
17. Proteomics in the Analysis of Prostate Cancer (pp. 277-298)
    Naaby-Hansen, Soren; Nagano, Kohji; Gaffney, Piers; Masters, John R. W.; Cramer, Rainer
18. Application of Gene Microarrays in the Study of Prostate Cancer (pp. 299-320)
    Nelson, Colleen C.; Hoffart, Douglas; Gleave, Martin E.; Rennie, Paul S.
19. Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific Enhancers Active in Prostate Cells (pp. 321-332)
    Watt, Fujiko; Molloy, Peter
20. Targeted Alpha Therapy of Prostate Cancer (pp. 333-358)
    Allen, Barry J.; Li, Yong; Rizvi, Syed M. A.; Russell, Pamela J.
21. Phenotypic and Functional Differences of Dendritic Cells Generated Under Different In Vitro Conditions (pp. 359-376)
    McArdle, Stephanie E. B.; Ali, Selman A.; Li, Geng; Mian, Shahid; Rees, Robert C.
22. Flavonoid Compounds in the Prevention and Treatment of Prostate Cancer (pp. 377-394)
    Kelly, Graham E.; Husband, Alan J.

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