Library Science

In Plant Gene Transfer and Expression Protocols, leading experts present the latest step-by-step recipes for introducing genes into model and agricultural plants and for studying gene activity using reporter enzyme assays, RNA techniques, and immunological methods. The book contains not only a complete collection of ??°mainstream??² methods, but also methods for studying gene expression and genetic manipulation in chloroplasts and mitochondria. There are also methods for introducing and analyzing plant genes in Xenopus and heterologous yeast hosts. Plant Gene Transfer and Expression Protocols is an indispensable tool for scientists working in both fundamental (biochemical, physiological, and genetic) and applied aspects of plant biotechnology, plant breeding, agriculture, and horticulture. It provides concise practical help that produces the desired results even for beginners and opens the doors to new research possibilities.

Authors: Jones, Heddwyn

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Table of Contents

1. Tools for Expressing Foreign Genes in Plants (pp. 1-32)
   Guerineau, Francois
2. Introduction of Cloning Plasmids into Agrobacterium tumefaciens (pp. 33-38)
   Shaw, Charles H.
3. Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco (pp. 39-48)
   Gallois, Patrick; Marinho, Paula
4. Agrobacterium rhizogenes as a Vector for Transforming Higher Plants: Application in Lotus corniculatus Transformation (pp. 49-62)
   Stougaard, Jens
5. Agrobacterium-Mediated Transformation of Arabidopsis thaliana: Application in T-DNA Tagging (pp. 63-76)
   Topping, Jennifer F.; Wei, Wenbin; Clarke, Michael C.; Muskett, Paul; Lindsey, Keith
6. Agrobacterium-Mediated Transfer of Geminiviruses to Plant Tissues (pp. 77-93)
   Boulton, Margaret I.
7. Stable Transformation of Barley via Direct DNA Uptake: Electroporation- and PEG-Mediated Protoplast Transformation (pp. 95-106)
   Lazzeri, Paul A.
8. Gene Transfer into Plant Protoplasts by Electroporation (pp. 107-112)
   Jones, Heddwyn
9. Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues (pp. 113-123)
   Barcelo, Pilar; Lazzeri, Paul A.
10. The ?÷-Glucuronidase (gus) Reporter Gene System Gene: Fusions; Spectrophotometric, Fluorometric, and Histochemical Detection (pp. 125-142)
    Hull, Gillian A.; Devic, Martine
11. Chloramphenicol Acetyl Transferase Assay (pp. 143-148)
    Davey, Michael R.; Blackhall, Nigel W.; Power, J. Brian
12. NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants (pp. 149-159)
    Curtis, Ian S.; Power, J. Brian; Davey, Michael R.
13. Gene Characterization by Southern Analysis (pp. 161-180)
    Sabelli, Paolo A.; Shewry, Peter R.
14. Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification (pp. 181-195)
    Offringa, Remko; van der Lee, Frederique
15. Isolation of Whole Cell (Total) RNA (pp. 197-202)
    Scott, Rod J.
16. Poly(A)+ RNA Isolation (pp. 203-206)
    Scott, Rod J.
17. In Vitro Translation (pp. 207-212)
    Scott, Rod J.
18. Northern Analysis and Nucleic Acid Probes (pp. 213-228)
    Sabelli, Paolo A.; Shewry, Peter R.
19. Nuclear ??°Run-On??² Transcription Assays (pp. 229-238)
    Gatehouse, John; Thompson, Andrew J.
20. RNase A/T1 Protection Assay (pp. 239-248)
    Simpson, Craig G.; Brown, John W. S.
21. Primer Extension Assay (pp. 249-256)
    Simpson, Craig G.; Brown, John W. S.
22. Applications of RT-PCR (pp. 257-270)
    Simpson, Craig G.; Brown, John W. S.
23. In Vitro Transcription of Class II Promoters in Higher Plants (pp. 271-290)
    Cooke, Richard; Penon, Paul
24. Analysis of Plant Gene Expression by Reverse Transcription-PCR (pp. 291-300)
    Bell, Mark
25. In Situ Hybridization to Plant Tissue Sections (pp. 301-316)
    Burgess, Shirley R.
26. Xenopus Oocytes as a Heterologous Expression System (pp. 317-340)
    Theodoulou, Frederica L.; Miller, Anthony J.
27. Heterologous Expression in Yeast (pp. 341-354)
    Trueman, Laurence J.
28. The Isolation of Intact Chloroplasts (pp. 355-360)
    Napier, Johnathan A.; Barnes, Simon A.
29. In Vitro Protein Import by Isolated Chloroplasts (pp. 361-368)
    Napier, Johnathan A.
30. Targeting of Foreign Proteins to the Chloroplast (pp. 369-376)
    Napier, Johnathan A.
31. Isolation of Mitochondria (pp. 377-382)
    Binder, Stefan; Grohmann, Lutz
32. Mitochondrial Nucleic Acid Purification and Analysis (pp. 383-390)
    Binder, Stefan
33. In organello Protein Synthesis (pp. 391-397)
    Grohmann, Lutz
34. Separation of Plant Proteins by Electrophoresis (pp. 399-422)
    Shewry, Peter R.; Tatham, Arthur S.; Fido, Roger J.
35. Western Blotting Analysis (pp. 423-438)
    Fido, Roger J.; Tatham, Arthur S.; Shewry, Peter R.
36. ELISA Detection of Foreign Proteins (pp. 439-452)
    Mills, E. N. Clare; Plumb, Geoffrey W.; Morgan, Michael R. A.
37. Immunocytochemical Localization of Proteins (pp. 453-462)
    Davies, John

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