Library Science

Peter Eigil Nielsen has assembled a critically evaluated collection of key PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process.

 Download Book Table of Contents

1. PNA Technology (pp. 03-26)
   Nielsen, Peter E.
2. Solid Phase Synthesis of PNA Oligomers (pp. 29-42)
   Beck, Frederik
3. Synthesis of PNA-Peptide Conjugates (pp. 43-52)
   Awasthi, Satish Kumar; Nielsen, Peter E.
4. Parallel Synthesis of PNA-Peptide Conjugate Libraries (pp. 53-58)
   Awasthi, Satish Kumar; Nielsen, Peter E.
5. Thermodynamics of PNA Interactions with DNA and RNA (pp. 59-88)
   Ratilainen, Tommi; Norden, Bengt
6. Measurement of PNA Binding to Double-Stranded DNA (pp. 91-110)
   Bentin, Thomas; Hansen, Georg I.; Nielsen, Peter E.
7. PNA-Mediated Immobilization of Supercoiled DNA (pp. 111-118)
   Bentin, Thomas; Nielsen, Peter E.
8. PNA Openers and Their Applications (pp. 119-130)
   Demidov, Vadim V.; Frank-Kamenetskii, Maxim D.
9. Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes (pp. 131-142)
   Wang, Miaomiao; Armitage, Bruce A.
10. PNA-Mediated PCR Clamping: Applications and Methods (pp. 145-164)
    Murdock, Deborah G.; Wallace, Douglas C.
11. PNA Clamping Techniques for the Determination of Oncogene Mutations (pp. 165-180)
    Schuermann, Marcus; Behn, Moira
12. PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis (pp. 181-194)
    Williams, Brett; Stender, Henrik; Coull, James M.
13. Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices (pp. 195-208)
    Igloi, Gabor L.
14. Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells (pp. 211-224)
    Braasch, Dwaine A.; Corey, David R.
15. Synthesis of Cell-Penetrating Peptide-PNA Constructs (pp. 225-236)
    Pooga, Margus; Soomets, Ursel; Bartfai, Tamas; Langel, Ulo
16. Antisense Inhibition of Bacterial Gene Expression and Cell Growth (pp. 237-248)
    Good, Liam
17. In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops (pp. 249-256)
    Mollegaard, Niels Erik; Nielsen, Peter E.
18. In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents (pp. 259-269)
    Kristensen, Edward

This entry was posted on Saturday, April 28th, 2007 at 4:05 pm and is filed under Molecular Biology. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.