Library Science

In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. Chapters also include a helpful Notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments. Dunn and Pennington’s Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

Authors: Dunn, Ben M.; Pennington, Michael W.

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Table of Contents

1. Gel-Filtration Chromatography (pp. 1-10)
   Bollag, Daniel M.
2. Ion-Exchange Chromatography (pp. 11-22)
   Bollag, Daniel M.
3. Reversed-Phase HPLC: Analytical Procedure (pp. 23-36)
   Nirenberg, Udo
4. Reversed-Phase High-Performance Liquid Chromatography: A Semipreparative Methodology (pp. 37-52)
   Byrnes, Michael E.
5. Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis (pp. 53-64)
   Crimmins, Dan L.
6. Principles and Practice of Peptide Analysis with Capillary Zone Electrophoresis (pp. 65-84)
   Wheat, Thomas E.
7. Fast Atom Bombardment Mass Spectrometric Characterization of Peptides (pp. 85-106)
   Das, P. R.; Pramanik, B. N.
8. Sequence Analysis of Peptide Resins from Boc/Benzyl Solid-Phase Synthesis (pp. 107-130)
   Pohl, Jan
9. NMR Spectroscopy of Peptides and Proteins (pp. 131-154)
   Hinds, Mark G.; Norton, Raymond S.
10. Techniques for Conjugation of Synthetic Peptides to Carrier Molecules (pp. 155-192)
    Carter, J. Mark
11. Epitope Prediction Methods (pp. 193-206)
    Carter, J. Mark
12. Epitope Mapping of a Protein Using the Geysen (PEPSCAN) Procedure (pp. 207-224)
    Carter, J. Mark
13. Analysis of Proteinase Specificity by Studies of Peptide Substrates: The Use of UV and Fluorescence Spectroscopy to Quantitate Rates of Enzymatic Cleavage (pp. 225-244)
    Dunn, Ben M.; Scarborough, Paula E.; Davenport, Ruth; Swietnicki, Wieslaw
14. Synthesis of Recombinant Peptides (pp. 245-260)
    Van Heeke, Gino; Stout, Jay S.; Wagner, Fred W.
15. De Novo Design of Proteins: Template-Assembled Synthetic Proteins (TASP) (pp. 261-286)
    Tuchscherer, Gabriele; Steiner, Verena; Altmann, Karl-Heinz; Mutter, Manfred
16. Chemical Synthesis of the Aspartic Proteinase from Human Immunodeficiency Virus (HIV) (pp. 287-304)
    Hoeprich, Paul D.
17. Multiple and Combinatorial Peptide Synthesis: Chemical Development and Biological Applications (pp. 305-328)
    Andrews, Philip C.; Leonard, Daniele M.; Cody, Wayne L.; Sawyer, Tomi K

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