Library Science

Hands-on laboratory experts present a set of “classic” PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

Authors: Sachse, Konrad; Frey, Joachim href=”http://www.blogsua.com/pdf/pcr-detection-of-microbial-pathogens-methods-and-protocols.pdf” target=”_blank”>Download Book

Table of Contents

1. Specificity and Performance of Diagnostic PCR Assays (pp. 03-30)
   Sachse, Konrad
2. Pre-PCR Processing of Samples (pp. 31-50)
   Radstrom, Peter; Knutsson, Rickard; Wolffs, Petra; Dahlenborg, Maria; Lofstrom, Charlotta
3. Critical Aspects of Standardization of PCR (pp. 51-64)
   Hoorfar, Jeffrey; Cook, Nigel
4. PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens (pp. 65-84)
   Lubeck, Peter Stephensen; Hoorfar, Jeffrey
5. Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae (pp. 87-96)
   Frey, Joachim
6. Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR (pp. 97-108)
   Ewalt, Darla R.; Bricker, Betsy J.
7. Isolation of Campylobacter and Identification by PCR (pp. 109-122)
   Englen, Mark D.; Ladely, Scott R.; Fedorka-Cray, Paula J.
8. Detection and Differentiation of Chlamydiae by Nested PCR (pp. 123-136)
   Sachse, Konrad; Hotzel, Helmut
9. Detection of Toxigenic Clostridia (pp. 137-152)
   Popoff, Michel R.
10. PCR-Based Detection of Coxiella burnetii from Clinical Samples (pp. 153-162)
    Berri, Mustapha; Arricau-Bouvery, Nathalie; Rodolakis, Annie
11. Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC) (pp. 163-184)
    Gallien, Peter
12. Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay (pp. 185-192)
    O’Connor, Louise
13. Detection of Leptospira interrogans (pp. 193-200)
    Lester, John W.; LeFebvre, Rance B.
14. Detection of Pathogenic Mycobacteria of Veterinary Importance (pp. 201-222)
    Skuce, Robin A.; Hughes, M. Siobhan; Taylor, Malcolm J.; Neill, Sydney D.
15. Multiplex PCR of Avian Pathogenic Mycoplasmas (pp. 223-230)
    Khan, Mazhar I.
16. Detection and Differentiation of Ruminant Mycoplasmas (pp. 231-246)
    Hotzel, Helmut; Frey, Joachim; Bashiruddin, John; Sachse, Konrad
17. Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air (pp. 247-256)
    Kobisch, Marylene; Frey, Joachim
18. PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida (pp. 257-274)
    Christensen, Henrik; Bisgaard, Magne; Larsen, Jesper; Olsen, John Elmerdahl
19. Detection of Salmonella spp. (pp. 275-288)
    Malorny, Burkhard; Helmuth, Reiner
20. Detection of Toxoplasma gondii (pp. 289-298)
    Wastling, Jonathan M.; Mattsson, Jens G.
21. PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples (pp. 299-310)
    Pozio, Edoardo; Rosa, Giuseppe La
22. Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure (pp. 311-324)
    Knutsson, Rickard; Radstrom, Peter

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