Library Science

Book Description

An indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. The techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation.

Authors: Deb, Sumitra; Deb, Swati Palit

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Table of Contents

1. Adenovirus Expressing p53 (pp. 1-16)
   Holmes, Matthew; Rosenberg, Elizabeth; Valerie, Kristoffer
2. Purification of Recombinant p53 from Sf9 Insect Cells (pp. 17-28)
   Sun, Xiu Zhu; Nguyen, John; Momand, Jamil
3. Generation and Characterization of p53 Mutant Mice (pp. 29-50)
   Dumble, Melissa L.; Donehower, Lawrence A.; Lu, Xiongbin
4. Identification of p53 Target Genes by Fluorescent Differential Display (pp. 51-64)
   Stein, Susanne; Cho, Yong-Jig; Jackson, Roger S.; Liang, Peng
5. p53-Induced Gene Expression Analysis (pp. 65-72)
   Sax, Joanna K.; El-Deiry, Wafik S.
6. Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR® Green I (pp. 73-92)
   Stagliano, Katherine E. Roth; Carchman, Evie; Deb, Sumitra
7. Transactivation and Transrepression Studies with p53 (pp. 93-110)
   Scian, Mariano J.; Frum, Rebecca; Deb, Swati Palit; Deb, Sumitra
8. Methods to Study p53-Repressed Promoters (pp. 111-120)
   Dumont, Patrick; Pietra, Anthony Della; Murphy, Maureen E.
9. Interaction of p53 with Cellular Proteins (pp. 121-134)
   Keller, David M.; Zeng, Shelya X.; Lu, Hua
10. Interaction of p53 with the Adenovirus E1B-55 kDa Protein (pp. 135-150)
    Roth, Judith; Dobbelstein, Matthias
11. In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability (pp. 151-170)
    Molina, Maria Patricia; Cain, Christine; Bargonetti, Jill
12. Signaling to p53: The Use of Phospho-Specific Antibodies to Probe for In Vivo Kinase Activation (pp. 171-202)
    Craig, Ashley L.; Bray, Susan E.; Finlan, Lee E.; Kernohan, Neil M.; Hupp, Ted R.
13. Detection of Mitochondrial Localization of p53 (pp. 203-210)
    Mihara, Motohiro; Moll, Ute M.
14. Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress (pp. 211-218)
    Joseph, Troy W.; Moll, Ute M.
15. Mutational Analysis of p53 in Human Tumors: Direct DNA Sequencing and SSCP (pp. 219-230)
    Erster, Susan; Slade, Neda; Moll, Ute M.
16. Mutational Analysis of p53 in Human Tumors: Immunocytochemistry (pp. 231-244)
    Slade, Neda; Moll, Ute M.
17. Flow Cytometric Analysis of p53-Induced Apoptosis (pp. 245-256)
    Berger, Michael; Haupt, Ygal
18. Flow Cytometric Analysis of MDM2-Mediated Growth Arrest (pp. 257-268)
    Frum, Rebecca; Deb, Swati Palit

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