Library Science

This comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research covers topics ranging from molecular and genetic techniques, to behavioral analyses of animal models and to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction.

Authors: Pan, Zhizhong Z.

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Table of Contents

1. Molecular Cloning of Opioid Receptors by cDNA Library Screening (pp. 3-16)
   Pan, Ying-Xian
2. Expression of Opioid Receptors in Mammalian Cell Lines (pp. 17-28)
   Pan, Ying-Xian
3. Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells (pp. 29-38)
   Thakker, Deepak R.; Ozsoy, Hatice Z.; Standifer, Kelly M.
4. Analysis of Opioid-Induced Kinase Activation (pp. 39-46)
   Ma, Lan
5. Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate (pp. 47-52)
   Wang, Jia Bei
6. Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice (pp. 53-64)
   Ikeda, Kazutaka; Yoshii, Mitsunobu; Sora, Ichiro; Kobayashi, Toru
7. Identification of Alternatively Spliced Variants from Opioid Receptor Genes (pp. 65-76)
   Pan, Ying-Xian
8. Immunohistochemical Localization of ??-, delta- and k-Opioid Receptors Within the Antinociceptive Brainstem Circuits (pp. 79-94)
   Kalyuzhny, Alexander E.
9. In Situ Hybridization in Neural Tissues (pp. 95-106)
   Gutstein, Howard B.
10. Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes (pp. 107-140)
    Silbert, Seth C.
11. Gene Arrays and Proteomics: A Primer (pp. 141-154)
    Mouledous, Lionel; Gutstein, Howard B.
12. Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors (pp. 157-184)
    Gomes, Ivone; Filipovska, Julija; Devi, Lakshmi A.
13. Recombinant Opioid Receptors: Structure-Function Relationship (pp. 185-204)
    Filipovska, Julija; Gomes, Ivone; Xu, Wei; Chen, Chongguang; Liu-Chen, Lee-Yuan; Devi, Lakshmi A.
14. Receptor Knock-Out and Gene Targeting: Generation of Knock-Out Mice (pp. 205-216)
    Sora, Ichiro; Ikeda, Kazutaka; Mishina, Yuji
15. Pharmacological Interventions at the Spinal Cord: Intrathecal Injections (pp. 217-222)
    Robinson, Daphne A.; Zhuo, Min
16. Opioid Tolerance in Adult and Neonatal Rats (pp. 223-232)
    Pan, Zhizhong Z.
17. Animal Models of Neuropathic Pain (pp. 233-242)
    Martin, William J.; Stewart, Laike St. A.; Tarpley, Jason W.
18. Place Conditioning to Study Drug Reward and Aversion (pp. 243-250)
    Carlezon, William A.
19. Opiate Self-Administration (pp. 251-264)
    Xi, Zheng-Xiong; Stein, Elliot A.
20. Acute, Chronic, and Cancer Pain: Clinical Management (pp. 267-283)
    Burton, Allen W.
21. Clinical Treatment of Opioid Addiction and Dependence (pp. 285-296)
    Ling, Walter; Rawson, Richard A.; Compton, Margaret

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