Library Science

Mark A. Findeis and a panel of active researchers present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact condensed phases that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents.

Authors: Findeis, Mark A.

Table of Contents

1. Synthesis of Polyampholyte Comb-Type Copolymers Consisting of Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier (pp. 1-9)
   Maruyama, Atsushi; Takei, Yoshiyuki
2. Cationic a-Helical Peptides for Gene Delivery into Cells (pp. 11-21)
   Niidome, Takuro; Aoyagi, Haruhiko
3. Supramolecular Self-Assembly of Poly(ethylene glycol)- Block-Poly(L-lysine) Dendrimer with Plasmid DNA (pp. 23-33)
   Choi, Joon Sig; Park, Jong Sang
4. Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery (pp. 35-60)
   Bos, Gert W.; Crommelin, Daan J. A.; Hennink, Wim E.
5. Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers (pp. 61-78)
   Oupicky, David; Read, Martin L.; Bettinger, Thierry
6. Use of Disulfide Cationic Lipids in Plasmid DNA Delivery (pp. 79-88)
   Tang, Fuxing; Hughes, Jeffrey A.
7. Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein (pp. 89-104)
   Rump, Erik T.; Biessen, Erik A. L.; van Berkel, Theo J. C.; Bijsterbosch, Martin K.
8. Coupling of Nuclear Localization Signals to Plasmid DNA (pp. 105-109)
   Neves, Carole; Scherman, Daniel; Wils, Pierre
9. Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery (pp. 111-121)
   Zhong, Bo-Hua; Wu, George Y.; Wu, Catherine H.
10. Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers (pp. 123-129)
    Takei, Yoshiyuki; Maruyama, Atsushi; Akaike, Toshihiro; Sato, Nobuhiro
11. Methods for Studying Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery (pp. 131-148)
    Read, Martin L.; Bettinger, Thierry; Oupicky, David
12. Characterization of DNA Condensates by Atomic Force Microscopy (pp. 149-158)
    Fang, Ye; Hoh, Jan H.
13. Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting (pp. 159-173)
    Scott, Rona S.; Vos, Jean-Michel H.
14. Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA (pp. 175-183)
    Horton, Holly M.; Parker, Suezanne E.
15. Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes (pp. 185-193)
    Horton, Holly M.; Parker, Suezanne E.
16. Nonviral DNA Delivery from Polymeric Systems (pp. 195-207)
    Shea, Lonnie D.; Mooney, David J.
17. Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers (pp. 209-224)
    Torigoe, Hidetaka; Maruyama, Atsushi
18. Lyophilization of Nonviral Gene Delivery Systems (pp. 225-252)
    Allison, S. Dean; Anchordoquy, Thomas J.
19. Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes (pp. 253-284)
    Braun, Chad S.; Kueltzo, Lisa A.; Middaugh, C. Russell
20. Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy (pp. 285-317)
    Choosakoonkriang, Sirirat; Wiethoff, Christopher M.; Kueltzo, Lisa A.; Middaugh, C. Russell
21. Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry (pp. 319-348)
    Lobo, Brian A.; Rogers, Sheila A.; Wiethoff, Christopher M.; Choosakoonkriang, Sirirat; Bogdanowich-Knipp, Susan; Middaugh, C. Russell
22. Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors (pp. 349-376)
    Wiethoff, Christopher M.; Middaugh, C. Russell
23. Renal Gene Therapy (pp. 377-391)
    Lien, Yeong-Hau H.; Lai, Li-Wen
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