Library Science

An outstanding collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular approaches to both normal and injured nervous system function, and particularly to neurodegenerative processes. Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. These powerful techniques will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. Ed. Harry, Jean ; Tilson, Hugh A.

Table of Contents

1. Introduction to Polymerase Chain Reaction (pp. 1-26)
   Miller, Mark C.; Cunningham, Laura
2. Methodological Issues in Polymerase Chain Reaction for RNA (pp. 27-36)
   Rasmussen, Alessandra D.
3. Subtractive Hybridization of cDNA Libraries (pp. 37-44)
   Kelner, Gregory S.; Maki, Richard A.
4. The Use of Differential Display RT-PCR for Identifying Altered Gene Expression in lschemic Brain Injury (pp. 45-52)
   Wang, Xinkang; Feuerstein, Giora Z.
5. Analysis of Gene Expression by Multiprobe RNase Protection Assay (pp. 53-66)
   Stalder, Anna K.; Pagenstecher, Axel; Kincaid, Carrie L.; Campbell, lain L.
6. Molecular Probes for PNS Neurotoxicity, Degeneration, and Regeneration (pp. 67-88)
   Toews, Arrel D.
7. Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis (pp. 89-98)
   Xiao, Yang; Pennypacker, Keith
8. Transfection of Mammalian Cells In Vitro: Use in Analysis of Neuronal Damage (pp. 99-104)
   Billingsley, Melvin L.; Thompson, Theresa A.
9. Detection of Apoptotic Cells in the Nervous System (pp. 105-124)
   Kagan, Terri; Zakeri, Zahra
10. Translocation Assays of Protein Kinase C Activation (pp. 125-132)
    Wescott, Ginger G.; Manring, Christina M.; Terrian, David M.
11. Astrocyte and Neuron Coculturing Method (pp. 133-144)
    Aschner, Michael; Bennett, Barbara A.
12. Viability Assays for Cells In Vitro: The Ethidium/Calcein Assay and the lmmunofluorescence Combination Assay (pp. 145-156)
    Thompson, Theresa A.
13. Measurement of Nitric Oxide Synthase Activity Using the Citrulline Assay (pp. 157-162)
    Ward, Thomas R.; Mundy, William R.
14. Measurement of lntraneuronal Free Calcium Using the Fluorescent Probe Technique (pp. 163-170)
    Kodavanti, Prasada Rao S.
15. Measurement of Calcium Buffering by Intracellular Organelles in Brain (pp. 171-176)
    Kodavanti, Prasada Rao S.
16. Receptor-Mediated Release of lnositolphosphates in Brain Slices (pp. 177-190)
    Tandon, Pushpa
17. The Use of Enzyme-Linked lmmunosorbent Assays to Quantitate Proteins in Biological Fluids (pp. 191-200)
    Germolec, Dori R.
18. Assays for the Analysis of Synaptic Proteins in Neurodegenerative Disorders (pp. 201-208)
    Masliah, Eliezer; Alford, Michael
19. Zymographic Method for the Measurement of Gelatinase Activity in Brain Tissue (pp. 209-218)
    Gottschall, Paul E.; Zhang, J. Wenjun; Deb, Suman
20. The Measurement of Monoamine Neurotransmitters in Microdialysis Perfusates Using HPLC-ECD (pp. 219-236)
    Acworth, Ian; Cunningham, Michael L.
21. Biochemical Measurement of Cholinesterase Activity (pp. 237-246)
    Padilla, Stephanie; Lassiter, T. Leon; Hunter, Deborah
22. Functional Study of Glutamate Receptor Channels in Brain Slices (pp. 247-256)
    Jones, Susan; Yakel, Jerrel L.
23. Selective Silver Staining for Neural Degeneration (pp. 257-270)
    Olin, Jeanene K.; Jensen, Karl F.
24. lmmunohistological Markers of Cerebrovascular Integrity (pp. 271-288)
    Jensen, Karl F.; Olin, Jeanene K.; Varner, Julie A.; lsaacson, Robert L.
25. Electron Probe X-Ray Microanalysis: A Quantitative Electron Microscopy Technique for Measurement of Elements and Water in Nervous Tissue Cells (pp. 289-300)
    LoPachin, Richard M.; Gaughan, Christopher L.

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