Library Science

Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.

Authors: Ed. Killeen, Anthony A.

Table of Contents

1. DNA Extraction from Paraffin-Embedded Tissues (pp. 1-4)
   Fan, Hongxin; Gulley, Margaret L.
2. DNA Extraction from Fresh or Frozen Tissues (pp. 5-10)
   Fan, Hongxin; Gulley, Margaret L.
3. RNA Extraction from Fresh or Frozen Tissues (pp. 11-14)
   Fan, Hongxin; Gulley, Margaret L.
4. Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53Gene (pp. 15-27)
   Vahakangas, Kirsi H.; Castren, Katariina; Welsh, Judith A.
5. Cleavase?? Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection (pp. 29-42)
   Heisler, Laura; Lee, Chao-Hung
6. Detection of Telomerase by In Situ Hybridization and by Polymerase Chain Reaction-Based Telomerase Activity Assay (pp. 43-58)
   Morales, Carmela P.; Holt, Shawn E.
7. Detection of Microsatellite Instability (pp. 59-71)
   Berg, Karin D.; Griffin, Constance A.; Eshleman, James R.
8. Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes (pp. 73-79)
   Pan, Langxing; Peng, Huaizheng
9. Fluorescent In Situ Hybridization: Evaluation for Ploidy and Gene Amplification (pp. 81-91)
   Sheldon, Susan
10. HER-2/ neuOncogene Amplification Determined by Fluorescence In Situ Hybridization (pp. 93-104)
    Ross, Jeffrey S.; Sheehan, Christine E.; Fletcher, Jonathan A.
11. A Nested Reverse Transcriptase-Polymerase Chain Reaction Assay to Detect BCR/ abl (pp. 105-114)
    Wasserman, Linda M.
12. Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemias (pp. 115-145)
    Viswanatha, David S.
13. Detection of t(14;18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin Lymphoma (pp. 147-163)
    Viswanatha, David S.
14. Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcriptase Polymerase Chain Reaction (pp. 165-175)
    Luke, Scott; Kaul, Karen L.
15. Molecular Detection of Circulating Prostate Cancer Cells (pp. 177-187)
    Kaul, Karen L.
16. Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias (pp. 189-209)
    Mitha, Naheed; McGlennen, Ronald C.
17. Monitoring of Bone Marrow Transplant Engraftment (pp. 211-225)
    Woronzoff-Dashkoff, Kristine P.; McGlennen, Ronald C.
18. Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1 (pp. 227-242)
    Petty, Elizabeth M.; Glynn, Michael; Bale, Allen E.
19. Molecular Detection of Multiple Endocrine Neoplasia Type 2 (pp. 243-262)
    Rowsell, Edward H.; Wick, Myra J.
20. Assay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis Coli Gene (pp. 263-270)
    Gruber, Stephen B.
21. Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization (pp. 271-300)
    Unger, Elizabeth R.; Vernon, Suzanne D.
22. Molecular Methods for Detecting Epstein-Barr Virus (Part I): In Situ Hybridization to Epstein-Barr Virus-Encoded RNA (EBER) Transcripts (pp. 301-311)
    Fan, Hongxin; Gulley, Margaret L.
23. Molecular Methods for Detecting Epstein-Barr Virus (Part II): Structural Analysis of Epstein-Barr Virus DNA as a Marker of Clonality (pp. 313-319)
    Fan, Hongxin; Gulley, Margaret L.
24. Molecular Methods for Detecting Epstein-Barr Virus (Part III): EBV Viral Load by Competitive Polymerase Chain Reaction (pp. 321-329)
    Fan, Hongxin; Gulley, Margaret L.
25. Molecular Detection of Kaposi???s Sarcoma-Associated Herpesvirus/ Human Herpesvirus-8 (pp. 331-336)
    Cesarman, Ethel
26. Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus (pp. 337-351)
    Scheuermann, Richard H.; Bai, Xin
27. A Colorimetric Microtiter Plate Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2 (pp. 353-362)
    Tang, Yi-Wei
28. Detection and Typing of Hepatitis C Virus (pp. 363-378)
    Nolte, Frederick S.
29. Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections (pp. 379-387)
    Mohl, Diana; Giordano, Thomas J.
30. Ultrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITOR??? Tests (pp. 389-412)
    Herman, Steven; Novotny, James; Rosenstraus, Maurice
31. Prenatal Genotyping of the RhD Locus to Identify Fetuses at Risk for Hemolytic Disease of the Newborn (pp. 427-437)
    Hessner, Martin J.; Bellissimo, Daniel B.
32. Molecular Diagnosis of Hereditary Hemochromatosis (pp. 439-450)
    Huber, Suzanne; Voelkerding, Karl V.
33. Genotyping of Apolipoprotein E (pp. 451-457)
    Ozturk, I. Cetin; Akel, Nahida; Killeen, Anthony A.
34. Genotyping for Functionally Important Human CYP2D64 (B) Mutation Using TaqMan Probes (pp. 459-472)
    Shi, Michael M.; Myrand, Scott P.; Bleavins, Michael R.; de la Iglesia, Felix A.

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