Library Science

A comprehensive and up-to-date set of basic and advanced methods that illuminates the principles of molecular diagnostics and allows researchers to establish assay systems tailored to their specific needs. This unique collection covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens and their use as diagnostic reagents in immunological assays. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors.Ed. Reischl, Udo Table of Contents

1. Identification of Novel Diagnostic Markers by Differential Display (pp. 3-13)
   Liang, Peng; Wang, Fellan; Zhu, Welmin; O?Connell, Robert P.; Averboukh, Lidia
2. lmmunoprecipitation (pp. 15-28)
   Johansen, Kari; Svensson, Lennart
3. RTPCR Methods and Applications (pp. 29-54)
   Siebert, Paul D.
4. Quantitative RT-PCR (pp. 55-80)
   Siebert, Paul D.
5. Cloning cDNA Ends Using RACE (pp. 81-106)
   Zhang, Yue; Frohman, Michael A.
6. A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains (pp. 107-124)
   Mierendorf, Robert C.; Hammer, Beth; Novy, Robert E.
7. Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis (pp. 125-142)
   Wienhues, Ulla; Kraas, Wolfgang; Seidel, Christoph; Ihlenfeldt, Hans Georg; Jung, Gunther
8. Amplification of Genomic DNA by PCR (pp. 143-156)
   Frey, Bruno
9. PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli (pp. 157-168)
   Reischl, Udo
10. Application of TEV Protease in Protein Production (pp. 169-184)
    Polayes, Deborah A.; Parks, T. Dawn; Johnston, Stephen A.; Dougherty, William G.
11. Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic, Therapeutic, and Preventive Purposes (pp. 185-200)
    Deml, Ludwig; Wagner, Ralf
12. Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked lmmunosorbent Assays (pp. 201-212)
    Warnes, Alan; Fooks, Anthony R.; Stephenson, John R.
13. Generation of Recombinant Baculovirus DNA in E. coli Using a Baculovirus Shuttle Vector (pp. 213-236)
    Ciccarone, Valentina C.; Polayes, Deborah A.; Luckow, Verne A.
14. The SFV Gene Expression System (pp. 237-256)
    Ciccarone, Valentina C.; Jessee, Joel A.; Liljestrom, Peter
15. Expression and Purification of Recombinant Proteins Using the pET System (pp. 257-292)
    Mierendorf, Robert C.; Morris, Barbara B.; Hammer, Beth; Novy, Robert E.
16. Procedures for the Analysis and Purification of His-Tagged Proteins (pp. 293-308)
    Kneusel, Richard E.; Crowe, Joanne; Wulbeck, Melanie; Ribbe, Joachim
17. Detection and Immobilization of Proteins Containing the 6xHis Tag (pp. 309-322)
    Kneusel, Richard E.; Wulbeck, Melanie; Ribbe, Joachim
18. The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins (pp. 323-330)
    Leong, Louis E. C.
19. Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) (pp. 331-344)
    Reischl, Udo
20. Purification of Recombinant Proteins Having High Isoelectric Points (pp. 345-360)
    Calogero, Raffaele A.; Aulicino, Anna
21. lmmunoblotting (pp. 361-372)
    Kirschfink, Michael; Terness, Peter
22. Diagnostic Application of a Multiple-Channel lmmunoblot Apparatus (pp. 373-396)
    Black, Shaun D.
23. Immobilization of lmmunoreactants on Solid Phases for ELlSAs (pp. 397-410)
    Decker, Richard; Leahy, David
24. Nonradioactive Labeling and lmmunodetection of Recombinant Proteins (pp. 411-426)
    Oberfelder, Robert
25. Flow Cytometry (pp. 427-442)
    Daniel, Volker
26. Basic Problems of Serological Laboratory Diagnosis (pp. 443-472)
    Fierz, Walter
27. Molecular Diagnostics Resources on the Internet (pp. 473-484)
    O?Donnell, Kevin J.
28. Monoclonal Antibody-Based lmmunoassays (pp. 487-502)
    Appleby, Peter; Reischl, Udo
29. Biosensor-Based Methods in Clinical Diagnosis (pp. 503-518)
    Aberl, Franz; Ko?linger, Conrad
30. The Quartz Crystal Microbalance (QCM) as an lmmunosensor (pp. 519-530)
    Aberl, Franz; Ko?linger, Conrad; Wolf, Hans
31. Biomolecular Interaction Analysis: Strategies for Antibody and Antigen Characterization (pp. 531-554)
    Roder, Uwe W.; Markey, Francis
32. Recent Developments in Antibody Engineering (pp. 555-580)
    Little, Melvyn; Dubel, Stefan; Kipriyanov, Sergey; Breitling, Frank
33. Cloning and Expression of Single-Chain Fragments (SCFV) from Mouse and Rat Hybridomas (pp. 581-592)
    Breitling, Frank; Dubel, Stefan
34. Production of a Human Antibody Library in the Phage-Display Vector pSEX81 (pp. 593-604)
    Welschof, Martin; Little, Melvyn; Dorsam, Heinz
35. Screening of Phage-Displayed Antibody Libraries (pp. 605-614)
    Dorsam, Heinz; Braunagel, Michael; Kleist, Christian; Moynet, Daniel; Welschof, Martin
36. Purification, Characterization, and Biotinylation of Single-Chain Antibodies (pp. 615-622)
    Kipriyanov, Sergey M.

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