Library Science

Leading clinicians and expert researchers bring together under one cover a comprehensive guide to the newest and most essential molecular techniques-particularly PCR-for diagnosing and monitoring hematological and solid tumors. These distinguished practitioners demonstrate step-by-step the use of PCR, FISH, CGH, Southern analysis, sequencing, and SSCP to assess cancer markers, to detect minimal disease and apoptosis, and to uncover particular translocations, mutations, and deletions. Their readily reproducible results offer physicians and scientists a promising entre into the powerful possibilities of using these techniques to improve the diagnosis, prognosis, and management of cancer.

Table of Contents

1. PCR of Gene Rearrangements for the Detection of Minimal Residual Disease in Childhood ALL (pp. 3-24)
   Goulden, Nick; Langlands, Kenneth; Steward, Colin; Knechtli, Chris; Potter, Mike; Oakhill, Tony
2. Detection of BCR-ABL in Hematological Malignancies by RT-PCR (pp. 25-36)
   Cross, Nicholas C. P.
3. Molecular Diagnosis of Acute Myeloid Leukemia with Maturation, FAB-Type M2 (pp. 37-46)
   Mensink, Ewald J. B. M.; van de Locht, Louis T. F.
4. Analysis of the PML/RAR-? Fusion Gene in Acute Promyelocytic Leukemia by Reverse-Transcription Polymerase Chain Reaction: Technical Recommendations, Advantages, and Pitfalls (pp. 47-54)
   Diverio, Daniela; Luciano, Anna; Riccioni, Roberta; Lo Coca, Francesco; Biondi, Andrea
5. RT-PCR Analysis of Breakpoints Involving the MLL Gene Located at 11q23 in Acute Leukemia (pp. 55-62)
   Pocock, Chris F. E.; Cotter, Finbarr E.
6. Polymerase Chain Reaction for Detection of the t(l4;18) Translocation in Lymphomas (pp. 63-74)
   Johnson, Peter W. M.
7. NPM-ALK Reverse Transcriptase-Polymerase Chain Reaction Analysis for Detection of the t(2;5) Translocation of Non-Hodgkin?s Lymphoma (pp. 75-90)
   Shurtleff, Sheila A.; Downing, James R.; Morris, Stephan W.
8. Molecular Diagnosis of the 5q Deletion in Malignant Myeloid Disorders (pp. 91-104)
   Boultwood, Jackie
9. Polymerase Chain Reaction Based Methods for Assessing Chimerism Following Allogeneic Bone Marrow Transplantation (pp. 105-120)
   Lawler, Mark; McCann, Shaun R.
10. Identification of Mutations in the Retinoblastoma Gene (pp. 123-140)
    Hogg, Annette
11. Mutational Analysis of the Wilms? Tumor (WT1) Gene (pp. 141-147)
    King-Underwood, Linda; Pritchard-Jones, Kathy
12. Single-Strand Conformation Polymorphism Mutation Analysis of the p53 Gene (pp. 151-160)
    Booth, Mark J.
13. The Characterization of Chromosomal Abnormalities Using Fluorescence In Situ Hybridization Procedures (pp. 161-182)
    Kempski, Helena M.
14. Comparative Genomic Hybridization (pp. 183-192)
    Williams, Briana J.
15. In Situ Hybridization of Cells and Tissue Sections (pp. 193-206)
    Conway, Simon J.
16. Apoptosis Detection by DNA Analysis (pp. 207-214)
    Allen, Paul D.; Newland, Adrian C.

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