Library Science

Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Authors: J. Boultwood, C. Fidler

Table of Contents

1. Molecular Analysis of Cancer: An Overview (pp. 001-005)
   Mills, Ken
2. Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization (pp. 007-027)
   Kearney, Lyndal; Tosi, Sabrina; Jaju, Rina J
3. Spectral Karyotyping in Cancer Cytogenetics (pp. 029-044)
   Hilgenfeld, Eva; Montagna, Cristina; Padilla-Nash, Hesed; Stapleton, Linda; Heselmeyer-Haddad, Kerstin; Ried, Thomas
4. Comparative Genomic Hybridization Analysis (pp. 045-057)
   Balsara, Binaifer R; Pei, Jianming; Testa, Joseph R
5. Detection of Chromosomal Deletions by Microsatellite Analysis (pp. 059-065)
   Ibbotson, Rachel E; Corcoran, Martin M
6. Detection and Quantification of Leukemia-Specific Rearrangements (pp. 067-096)
   Hochhaus, Andreas
7. Detection of t(2;5)(p23;q35) Translocation by Long-Range PCR of Genomic DNA (pp. 097-105)
   Jiang, Yunfang; Medeiros, L Jeffrey; Sarris, Andreas H
8. Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer (pp. 107-114)
   Sirivatanauksorn, Vorapan; Sirivatanauksorn, Yongyut; McKie, Arthur B; Lemoine, Nicholas R
9. Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism: Lessons from the ATM Gene (pp. 115-124)
   Vorechovsky, Igor
10. Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis (pp. 125-139)
    Guldberg, Per; Gronb?k, Kirsten; Worm, Jesper; Straten, Per thor; Zeuthen, Jesper
11. Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay (pp. 141-155)
    Goldrick, Marianna; Prescott, James
12. Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay (pp. 157-170)
    Side, Lucy
13. Mutation Analysis of Cancer Using Automated Sequencing (pp. 171-177)
    Strickson, Amanda; Fidler, Carrie
14. Detection of Differentially Expressed Genes in Cancer Using Differential Display (pp. 179-193)
    Fu, Yineng
15. Genomewide Gene Expression Analysis Using cDNA Microarrays (pp. 195-204)
    Kong, Chuang Fong; Bowtell, David
16. Gene Expression Profiling in Cancer Using cDNA Microarrays (pp. 205-222)
    Khan, Javed; Saal, Lao H; Bittner, Michael L; Jiang, Yuan; Gooden, Gerald C; Glatfelter, Arthur A; Meltzer, Paul S
17. Wilms Tumor Gene WT1as a Tumor Marker for Leukemic Blast Cells and Its Role in Leukemogenesis (pp. 223-237)
    Sugiyama, Haruo
18. Detection of Aberrant Methylation of the p15INK4B Gene Promoter (pp. 239-249)
    Uchida, Toshiki
19. Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon (pp. 251-270)
    Phelan, John T; Prchal, Josef T
20. Telomere Length Changes in Human Cancer (pp. 271-278)
    Broccoli, Dominique; Godwin, Andrew K
21. Measurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic Disorders (pp. 279-299)
    Ohyashiki, Kazuma; Ohyashiki, Junko H
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