Library Science

Leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a wide range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts’ genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. These cutting-edge methods offer investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms.

Authors: Miller, Wolfgang J.; Capy, Pierre

Table of Contents

1. Mobile Genetic Elements as Natural Tools for Genome Evolution (pp. 001-020)
   Miller, Wolfgang J.; Capy, Pierre
2. Detection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization (pp. 021-028)
   Biemont, Christian; Monti-Dedieu, Laurence; Lemeunier, Francoise
3. Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila (pp. 029-040)
   Dimitri, Patrizio
4. Southern Blot Analysis of Individual Drosophila Flies (pp. 041-058)
   Junakovic, Nikolaj
5. Computational Analysis of Transposable Element Sequences (pp. 059-072)
   Jordan, I. King; Bowen, Nathan J.
6. Biochemical Analysis of Long Terminal Repeat Retrotransposons (pp. 073-082)
   Andreaschmair, Andreaschmair; Garber, Karin; Takeda, Shin; Sugimoto, Kazuhiko; Kakutani, Tetsuji; Hirochika, Hirohiko
7. Tn5 as a Molecular Genetics Tool: InVitro Transposition and the Coupling of In Vitro Technologies With In Vivo Transposition (pp. 083-096)
   Reznikoff, William S.; Goryshin, Igor Y.; Jendrisak, Jerry J.
8. Gene Targeting in Drosophila (pp. 097-114)
   Gloor, Gregory B.
9. TE-Based Mutagenesis Systems in Plants: A Gene Family Approach (pp. 115-128)
   Vandenbussche, Michiel; Gerats, Tom
10. The Use of Double-Stranded RNA to Knock Down Specific Gene Activity (pp. 129-144)
    Montgomery, Mary K.
11. The Application of LTR Retrotransposons as Molecular Markers in Plants (pp. 145-174)
    Schulman, Alan H.; Flavell, Andrew J.; Ellis, T. H. Noel
12. MITE Display (pp. 175-188)
    Casa, Alexandra M.; Nagel, Alexander; Wessler, Susan R.
13. Retroposon Mapping in Molecular Systematics (pp. 189-226)
    Okada, Norihiro; Shedlock, Andrew M.; Nikaido, Masato
14. Transformation Systems in Insects (pp. 227-254)
    O’Brochta, David A.; Atkinson, Peter W.
15. Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental Strategies (pp. 255-276)
    Ivics, Zoltan; Izsvak, Zsuzsanna

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