Library Science

A collection by leading experts detail of readily reproducible methods for identifying polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP 2, C2, C4, and TNF- a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles.

mhc-protocols.pdf Table of Contents

1. HLA Informatics: Accessing HLA Sequences from Sequence Databases (pp. 03-22)
   Robinson, James; Marsh, Steven G. E.
2. Accessing HLA Sequencing Data Through the 6ace Database (pp. 23-42)
   Horton, Roger; Beck, Stephan
3. HLA Typing by Restriction Fragment Length Polymorphism Analysis (pp. 45-60)
   Vaughan, Robet W.
4. PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles (pp. 61-66)
   Vaughan, Robert W.
5. PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR (pp. 67-112)
   Middleton, Derek; Williams, F.
6. HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes (pp. 113-142)
   Steiner, Lori L.; Moonsamy, Priscilla V.; Bugawan, Teodorica L.; Begovich, Ann B.
7. PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles (pp. 143-172)
   Bunce, Mike
8. HLA Typing With Reference Strand-Mediated Conformation Analysis (pp. 173-190)
   Arguello, J. Rafael; Perez-Rodriguez, Martha; Pay, Andrea; Fisher, Gaby; McWhinnie, Alasdair; Madrigal, J. Alejandro
9. Sequencing Protocols for Detection of HLA Class I Polymorphism (pp. 191-222)
   Dunn, Paul P. J.; Cox, Steven T.; Little, Ann-Margaret
10. HLA-E and HLA-G Typing (pp. 223-236)
    Martinez-Laso, Jorge; Gomez-Casado, Eduardo; Arnaiz-Villena, Antonio
11. Typing Alleles of HLA-DM (pp. 237-246)
    Teisserenc, Helene
12. Typing Alleles of TAP1 and TAP2 (pp. 249-258)
    Powis, Stephen H.
13. Determining Alleles of the C2 Gene by Southern Blotting (pp. 259-268)
    Zhu, Zeng-Bian; Volanakis, John E.
14. Complement C4 Protein and DNA Typing Methods (pp. 269-296)
    Schneider, Peter M.; Mauff, Gottfried
15. Typing of Tumor Necrosis Factor Alleles (pp. 297-304)
    Wilson, Anthony Gerard
16. Molecular Typing of the MHC Class I Chain-Related Gene Locus (pp. 305-322)
    Collins, R. W. M.; Stephens, Henry A. F.; Vaughan, Robert W.
17. HLA Microsatellite Analysis (pp. 325-332)
    Carrington, Mary

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