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Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.

Authors: Brooks, Susan A., Schumacher, Udo

Table of Contents

1. Cell Separations by Flow Cytometry (pp. 003-015)
   Davies, Derek
2. Immunomagnetic Cell Separation (pp. 017-023)
   Clarke, Catherine; Davies, Susan
3. Genetic Modification of Cell Lines to Enhance Their Metastatic Capability (pp. 025-030)
   McWilliams, Daniel; Collins, Hilary
4. Cell Aggregation Assays (pp. 033-045)
   Boterberg, Tom; Bracke, Marc E; Bruyneel, Erik A; Mareel, Marc M
5. Cell Migration and the Boyden Chamber (pp. 047-054)
   Brown, Nicholas S; Bicknell, Roy
6. Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay (pp. 055-060)
   Niinaka, Yasufumi; Haga, Arayo; Raz, Avraham
7. In Vitro Invasion Assay Using Matrigel?? (pp. 061-070)
   Hall, Debbie M S; Brooks, Susan A
8. Membrane Invasion Culture System (pp. 071-079)
   Hendrix, Mary J C; Seftor, Elisabeth A; Seftor, Richard E B
9. Collagen Invasion Assay (pp. 081-089)
   Bracke, Marc E; Boterberg, Tom; Bruyneel, Erik A; Mareel, Marc M
10. Chick Heart Invasion Assay (pp. 091-102)
    Bracke, Marc E; Boterberg, Tom; Mareel, Marc M
11. Adhesion of Tumor Cells to Matrices and Endothelium (pp. 103-117)
    Rainger, G Ed
12. Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay (pp. 119-124)
    Jones, Adam; Fujiyama, Chisato; Hague, Stephen; Bicknell, Roy
13. Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis (pp. 125-148)
    Kilic, Nerbil; Ergun, Suleyman
14. Galectin-3 Binding and Metastasis (pp. 149-157)
    Nangia-Makker, Pratima; Honjo, Yuichiro; Raz, Avraham
15. Basic Principles for the Study of Metastasis Using Animal Models (pp. 161-171)
    Eccles, Suzanne A
16. The Chick Embryo Metastasis Model (pp. 173-187)
    Petitclerc, Eric; Brooks, Peter C
17. Gene Transfection for Metastasis Research Using Animal Models (pp. 189-193)
    Muschel, Ruth J; Hua, Jin
18. Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice (pp. 195-204)
    Watson, Sue A; Morris, Teresa M
19. Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastasis Models (pp. 205-213)
    Price, Janet E
20. Xenograft Models in Immunodeficient Animals: II. The Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis (pp. 215-222)
    Schumacher, Udo; Brooks, Susan A
21. Syngeneic Murine Metastasis Models: B16 Melanoma (pp. 223-229)
    Giavazzi, Raffaella; Garofalo, Angela
22. Transgenic Animal Models (pp. 231-249)
    Guy, Chantale T; Cardoso, Gizela
23. Clinically Accurate Orthotopic Mouse Models of Cancer (pp. 251-275)
    Hoffman, Robert M
24. Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo (pp. 277-284)
    Rocha, Marian; Schirrmacher, Volker; Umansky, Victor
25. Green Fluorescent Protein for Metastasis Research (pp. 285-298)
    Hoffman, Robert M

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