Library Science

Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. The work focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, and differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models of metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.

Authors: Brooks, Susan A., Schumacher, Udo

Table of Contents

1. Histopathological Assessment of Metastasis (pp. 3-11)
   Roskell, Derek E.; Buley, Ian D.
2. Basic Immunocytochemistry for Light Microscopy (pp. 13-39)
   Brooks, Susan A.
3. Multiple Labeling Techniques for Fluorescence Microscopy (pp. 41-48)
   Atherton, Amanda J.; Clarke, Catherine
4. Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues (pp. 49-65)
   Brooks, Susan A.; Hall, Debbie M. S.
5. Immunocytochemical Detection and Characterization of Individual Micrometastatic Tumor Cells (pp. 67-73)
   Braun, Stephan; Pantel, Klaus
6. Understanding Metastasis Through Integrin Expression (pp. 75-98)
   John, Lawrence; Pignatelli, Massimo
7. Surrogate Markers of Angiogenesis and Metastasis (pp. 99-113)
   Ranieri, Girolamo; Gasparini, Giampietro
8. Measurement of Microvessel Density in Primary Tumors (pp. 115-121)
   Turner, Helen E.; Harris, Adrian L.
9. Assessment of Cellular Proliferation by Calculation of Mitotic Index and by Immunohistochemistry (pp. 123-131)
   Gillett, Cheryl E.
10. Flow Cytometric Measurement of Cell Proliferation (pp. 133-143)
    Camplejohn, Richard S.
11. SDS-PAGE and Western Blotting Techniques (pp. 145-162)
    Blancher, Christine; Jones, Adam
12. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography (pp. 163-174)
    Toth, Marta; Fridman, Rafael
13. In Situ Hybridization to Localize mRNAs (pp. 177-197)
    Poulsom, Richard
14. Fluorescence In Situ Hybridization (pp. 199-221)
    Goker, Hakan; Shipley, Janet
15. Comparative Genomic Hybridization (pp. 223-240)
    Roylance, Rebecca
16. RNA Purification and Quantification Methods (pp. 241-253)
    Jones, Adam; Fujiyama, Chisato; Smith, Kenneth
17. Northern and Southern Blotting and the Polymerase Chain Reaction to Detect Gene Expression (pp. 255-270)
    Tennant, Thelma; Chekmareva, Marina; Rinker-Schaeffer, Carrie W.
18. Methylation Analysis of CpG Islands (pp. 271-283)
    Lilischkis, Richard; Kneitz, Hermann; Kreipe, Hans
19. Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction (pp. 285-295)
    Haack, Katja; Soondrum, Krishna; Neumaier, Michael
20. Differential Display (pp. 297-305)
    Kao, Ruey Ho; Francia, Giulio
21. Predictive Mathematical Modeling in Metastasis (pp. 309-315)
    Sherratt, Jonathan A.

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