Library Science

Andrew Pollard and Martin Maiden bring together a panel of leading authorities to produce a comprehensive, interdisciplinary survey of recent advances and knowledge about meneigococcal disease. The review and methods-based chapters collected here provide essential information for diagnosis in the clinical microbiology laboratory, isolate characterization, clinical management, and control of meningococcal disease. They also examine the immunopathological mechanisms occurring in the acutely ill, host???pathogen interactions, and the possible components of meningococcus responsible for virulence. Meningococcal Disease: Methods and Protocols integrates the basic science, clinical, and epidemiological aspects of this terrible infectious disease to promote the deeper understanding needed to identify novel targets for therapeutic interventions and vaccines.

Authors: Pollard, Andrew J. ; Maiden, Martin C.J.

Table of Contents

1. Microbiology and Laboratory Diagnosis (pp. 1-8)
   Cartwright, Keith
2. Isolation, Culture, and Identification of Meningococci from Clinical Specimens (pp. 9-21)
   Olcen, Per; Fredlund, Hans
3. PCR Diagnosis (pp. 23-39)
   Guiver, Malcolm; Borrow, Ray
4. Detection of Meningococcal Antigen by Latex Agglutination (pp. 41-59)
   Sobanski, Michael A.; Barnes, Rosemary A.; Coakley, W. Terence
5. Meningococcal Serology (pp. 61-87)
   Gray, Stephen J.; Borrow, Ray; Kaczmarski, Edward B.
6. Antibiotic Susceptibility Testing (pp. 89-106)
   Block, Colin
7. Molecular Approach for the Study of Penicillin Resistance In Neisseria meningitidis (pp. 107-119)
   Arreaza, Luisa; Vazquez, Julio A.
8. Overview: Epidemiology, Surveillance, and Population Biology (pp. 121-130)
   Maiden, Martin C. J.; Begg, Norman T.
9. Serological Characterization (pp. 131-145)
   Kuipers, Betsy; van den Dobbelsteen, Germie; Wedege, Elisabeth; van Alphen, Loek
10. Pulsed-Field Gel Electrophoresis (pp. 147-155)
    Achtman, Mark; Morelli, Giovanna
11. Nucleotide Sequencing of Antigen Genes of Neisseria meningitidis (pp. 157-172)
    Urwin, Rachel
12. Multi-Locus Sequence Typing (pp. 173-186)
    Jolley, Keith A.
13. Capsular Operons (pp. 187-201)
    Vogel, Ulrich; Claus, Heike; Frosch, Matthias
14. Molecular Analysis of the Meningococcal LPS Expression (pp. 203-214)
    Jennings, Michael P.; Berrington, Andrew W.; Moxon, E. Richard
15. Genome Sequencing and Annotation (pp. 215-230)
    Jeffries, Alex C.; Saunders, Nigel J.; Hood, Derek W.
16. Representational Difference Analysis (pp. 231-255)
    Bowler, Lucas D.; Bart, Aldert; van der Ende, Arie
17. Managing Outbreaks: The Public Health Response (pp. 257-272)
    Stuart, James M.
18. Global Trends in Meningococcal Disease (pp. 273-292)
    Caugant, Dominique A.
19. Epidemiology, Surveillance, and Population Biology: Carriage Studies (pp. 293-311)
    Cartwright, Keith
20. Surveillance of Meningococcal Disease in Europe (pp. 313-332)
    Noah, Norman
21. Global Surveillance for Meningococcal Disease (pp. 333-339)
    Rosenstein, Nancy E.
22. Epidemiology of Meningococcal Disease in North America (pp. 341-356)
    Pollard, Andrew J.; Scheifele, David; Rosenstein, Nancy
23. Computational Methods for Meningococcal Population Studies (pp. 357-373)
    Jolley, Keith A.; Urwin, Rachel
24. Clinical Studies: An Overview (pp. 375-410)
    Riordan, F. Andrew I.; Pollard, Andrew J.
25. Clinical Scoring Systems in Meningococcal Disease (pp. 411-425)
    Festa, Marino; Derkx, Bert
26. Quantitative Detection of Bacterial Lipopolysaccharides in Clinical Specimens (pp. 427-439)
    Brandtzaeg, Petter; Ovstebo, Reidun; Kierulf, Peter
27. Isolation and Analysis of Radiolabeled Meningococcal Endotoxin (pp. 441-458)
    Giardina, Peter C.; Weiss, Jerrold A.; Gibson, Brad W.; Apicella, Michael A.
28. Cytokine Measurement In Vivo and In Vitro (pp. 459-486)
    van Deuren, Marcel; Brandtzaeg, Petter
29. Cytokine Bioassays (pp. 487-497)
    Liabakk, Nina-Beate; Waage, Anders; Lien, Egil; Stenvik, Jorgen; Espevik, Terje
30. Molecular Analysis of Gene-Polymorphisms Affecting the Host Response to Infection (pp. 499-512)
    Westendorp, Rudi G. J.; Huizinga, Tom W. J.
31. Coagulation Studies (pp. 513-528)
    Hazelzet, Jan A.; Hack, C. Erik; de Groot, Ronald
32. Complement Pathways and Meningococcal Disease: Diagnostic Aspects (pp. 529-548)
    Sjoholm, Anders G; Truedsson, Lennart; Jensenius, Jens C.
33. Evaluation of New Treatments for Meningococcal Disease (pp. 549-586)
    Nadel, Simon; Macintosh, Iain; Levin, Michael
34. Studies on the Effect of Neisserial Porins on Apoptosis of Mammalian Cells (pp. 587-598)
    Massari, Paola; Wetzler, Lee M.
35. Molecular Recognition Mechanisms of Meningococci: Identifying Receptor-Ligand Pairs Involved in Microbe-Host Interactions (pp. 599-620)
    Virji, Mumtaz; Serino, Laura
36. Experimental Nasopharyngeal Colonization by Neisseria meningitidis using Explant Organ Culture (pp. 621-634)
    Read, Robert C.; Goodwin, Linda
37. Invasion of the Central Nervous System by Neisseria meningitidis (pp. 635-648)
    Tinsley, Colin; Nassif, Xavier
38. Interactions of Meningococci with Endothelium (pp. 649-662)
    Heyderman, Robert S.; Klein, Nigel J.
39. A Promoter Probe Plasmid Based on Green Fluorescent Protein: A Strategy for Studying Meningococcal Gene Expression (pp. 663-678)
    Webb, Steven A. R.; Langford, Paul R.; Kroll, J. Simon
40. Signature-Tagged Mutagenesis (pp. 679-692)
    Bakshi, S.; Sun, Y.-H.; Chalmers, R.; Tang, C. M.
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