Library Science

An outstanding collection of both classic and cutting-edge techniques for the detection and measurement of mitogen-activated protein kinase (MAPK) signaling cascades. The protocols includes methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the down regulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. The techniques offer biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed in to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

Ed. Seger, Rony

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Table of Contents

1. The ERK Cascade As a Prototype of MAPK Signaling Pathways (pp. 1-28)
   Rubinfeld, Hadara; Seger, Rony
2. Determination of ERK Activity: Antiphospho-ERK Antibodies, In Vitro Phosphorylation, and In-Gel Kinase Assay (pp. 29-48)
   Kraus, Sarah; Seger, Rony
3. Detection of ERK1/2 Activities Using Affinity Reagents (pp. 49-60)
   Pulido, Rafael; Zuniga, Angel; Ullrich, Axel
4. Activation of SAPKs/JNKs and p38s In Vitro (pp. 61-88)
   Kyriakis, John M.; Liu, Hong; Chadee, Deborah N.
5. Investigating the Cellular BMK1/ERK5 Signaling Pathway (pp. 89-96)
   Tapping, Richard I.; Yutaka, Kato; Chao, Ta-Hsiang; Hayashi, Masaaki; Lo, Jeng-Fan; Kim, Sung-Woo; Lee, Jiing-Dwan
6. Pull-Down Assays for Guanoside 5’-Triphosphate-Bound Ras-Like Guanosine 5’-Triphosphatases (pp. 97-102)
   van Triest, Miranda; Bos, Johannes L.
7. Regulation of MAPK Cascades by Protein Tyrosine Phosphatases (pp. 103-112)
   Torres, Josema; Blanco-Aparicio, Carmen; Pulido, Rafael
8. Use of Inhibitors in the Study of MAPK Signaling (pp. 113-126)
   Shaul, Yoav D.; Seger, Rony
9. Structure of MAPKs (pp. 127-144)
   Goldsmith, Elizabeth J.; Cobb, Melanie H.; Chang, Chung-I
10. Subcellular Localization of MAPKs (pp. 145-154)
    Adachi, Makoto; Nishida, Eisuke
11. Study of MAPK Signaling Using Knockout Mice (pp. 155-166)
    Pages, Gilles; Pouyssegur, Jacques
12. Computer Simulation of MAPK Signal Transduction (pp. 167-176)
    Aguda, Baltazar D.; Sauro, Herbert M.
13. Signaling by Growth Factor Receptors (pp. 177-202)
    Kochupurakkal, Bose S.; Yarden, Yosef
14. Activation of MAPKs by G Protein-Coupled Receptors (pp. 203-210)
    Crespo, Piero; Gutkind, J. Silvio
15. Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation (pp. 211-236)
    Fukunaga, Rikiro; Hunter, Tony
16. Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries (pp. 237-250)
    Yaffe, Michael B.
17. Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells (pp. 251-262)
    Aronheim, Ami
18. Methods in Functional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients In-Gel Digestion and Identification of Proteins by Mass Spectrometry (pp. 263-282)
    Bernard, Karine R.; Jonscher, Karen R.; Resing, Katheryn A.; Ahn, Natalie G.
19. Practical Methods for Deuterium Exchange/Mass Spectrometry (pp. 283-298)
    Hoofnagle, Andrew N.; Resing, Katheryn A.; Ahn, Natalie G.
20. Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling (pp. 299-314)
    Perdiguero, Eusebio; Nebreda, Angel R.
21. MAPK Cascades in the Brain: Lessons From Learning (pp. 315-322)
    Berman, Diego E.; Dudai, Yadin

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