Library Science

Internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).

Authors: Doolan, Denise L.

Table of Contents

1. Vector Incrimination and Entomological Inoculation Rates (pp. 01-12)
   Beier, John C.
2. Epidemiological Measures of Risk of Malaria (pp. 13-22)
   Baird, J. Kevin; Bangs, Michael J.; Maguire, Jason D.; Barcus, Mazie J.
3. Maintenance of the Plasmodium berghei Life Cycle (pp. 25-40)
   Sinden, Robert E.; Butcher, Geoff A.; Beetsma, A. L.
4. Mouse Models for Pre-Erythrocytic-Stage Malaria (pp. 41-56)
   Renia, Laurent; Belnoue, Elodie; Landau, Ire
5. Mouse Models for Erythrocytic-Stage Malaria (pp. 57-76)
   Sanni, Latifu A.; Fonseca, Luis F.; Langhorne, Jean
6. Nonhuman Primate Models: I. Nonhuman Primate Host-Parasite Combinations (pp. 77-84)
   Collins, William E.
7. Nonhuman Primate Models: II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax (pp. 85-92)
   Collins, William E.
8. Vector Analysis (pp. 95-102)
   Beier, John C.
9. Genotyping of Plasmodium spp.: Nested PCR (pp. 103-116)
   Snounou, Georges
10. Genotyping of Plasmodium falciparum: PCR-RFLP Analysis (pp. 117-129)
    Felger, Ingrid; Beck, Hans-Peter
11. Microsatellite Analysis in Plasmodium falciparum (pp. 131-136)
    Su, Xin-zhuan; Ferdig, Michael T.
12. Quantitation of Liver-Stage Parasites by Automated TaqMan?? Real-Time PCR (pp. 137-140)
    Witney, Adam A.; Anthony, Robert M.; Carucci, Daniel J.
13. Quantitation of Liver-Stage Parasites by Competitive RT-PCR (pp. 141-148)
    McKenna, Kyle C.; Briones, Marcelo R. S.
14. Extraction and Purification of Plasmodium Total RNA (pp. 151-158)
    Voss, Till
15. Extraction and Purification of Plasmodium Parasite DNA (pp. 159-164)
    Beck, Hans-Peter
16. Southern Blotting of Parasite DNA (pp. 165-176)
    Spielmann, Tobias
17. SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins (pp. 177-188)
    Cooper, Roland A.
18. Nested PCR Analysis of Plasmodium Parasites (pp. 189-204)
    Snounou, Georges; Singh, Balbir
19. RFLP Analysis (pp. 205-212)
    Beck, Hans-Peter
20. Analysis of Gene Expression by RT-PCR (pp. 213-224)
    Preiser, Peter
21. In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites (pp. 225-234)
    Thompson, Joanne
22. Purification of Chromosomes from Plasmodium falciparum (pp. 235-240)
    Carucci, Daniel J.; Horrocks, Paul; Gardner, Malcolm J.
23. Construction of Genomic Libraries from the DNA of PlasmodiumSpecies (pp. 241-252)
    Cummings, Leda M.; Rathore, Dharmendar; McCutchan, Thomas F.
24. Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA (pp. 253-264)
    Rathore, Dharmendar; McCutchan, Thomas F.
25. Construction of Plasmodium falciparum lambda cDNA Libraries (pp. 265-275)
    Fidock, David A.; Rathore, Dharmendar; McCutchan, Thomas F.
26. Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization (pp. 277-290)
    Fidock, David A.; Nguyen, Thanh V.; Beerntsen, Brenda T.; James, Anthony A.
27. Construction and Screening of YAC Libraries (pp. 291-304)
    Sanchez, Cecilia P.; Preuss, Martin; Lanzer, Michael
28. Episomal Transformation of Plasmodium berghei (pp. 305-316)
    Janse, Chris J.; Waters, Andrew P.
29. Gene Targeting in Plasmodium berghei (pp. 317-331)
    Thathy, Vandana; Menard, Robert
30. Peptide Vaccination (pp. 335-346)
    Meraldi, Valentin; Romero, Jackeline F.; Corradin, Giampietro
31. DNA Vaccination (pp. 347-360)
    Hedstrom, Richard C.; Doolan, Denise L.
32. Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses (pp. 361-367)
    Tsuji, Moriya
33. Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation (pp. 369-383)
    Quakyi, Isabella A.; Ahlers, Jeffrey D.
34. Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice (pp. 385-400)
    Seixas, Elsa; Langhorne, Jean; Quin, Stuart
35. Cell Trafficking: Malaria Blood-Stage Parasite-Specific (pp. 401-408)
    Hirunpetcharat, Chakrit; Good, Michael F.
36. Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry (pp. 409-422)
    Allsopp, Catherine E. M.; Langhorne, Jean
37. Cytokine Analysis by Intracellular Staining (pp. 423-436)
    Ansari, Aftab A.; Mayne, Ann E.
38. Assessment of Antigen-Specific CTLand CD8+-Dependent IFN-y Responses in Mice (pp. 437-444)
    Peter, Katrin; Audran, Regine; Bonelo, Anilza; Corradin, Giampietro; Lopez, Jose Alejandro
39. Assessing Antigen-Specific CD8+ CTL Responses in Humans (pp. 445-456)
    Doolan, Denise L.
40. Human Antibody Subclass ELISA (pp. 457-460)
    Druilhe, Pierre; Bouharoun-Tayoun, Hasnaa
41. Systemic Nitric Oxide Production in Human Malaria: I. Analysis of NO Metabolites in Biological Fluids (pp. 461-468)
    Anstey, Nicholas M.; Boutlis, Craig S.; Saunders, Jocelyn R.
42. Systemic Nitric Oxide Production in Human Malaria: II. Analysis of Mononuclear Cell Nitric Oxide Synthase Type 2 Antigen Expression (pp. 469-474)
    Saunders, Jocelyn R.; Misukonis, Mary A.; Weinberg, J. Brice; Anstey, Nicholas M.
43. In Vitro Culture of Plasmodium Parasites (pp. 477-488)
    Jensen, James B.
44. Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator (pp. 489-498)
    Haynes, J. David; Moch, J. Kathleen
45. Hepatic Portal Branch Inoculation (pp. 499-502)
    Sacci, John B.
46. Hepatocyte Perfusion, Isolation, and Culture (pp. 503-506)
    Sacci, John B.
47. Inhibition of Sporozoite Invasion: The Double-Staining Assay (pp. 507-516)
    Renia, Laurent; Vigario, Ana Margarida; Belnoue, Elodie
48. nhibition of Liver-Stage Development Assay (pp. 517-520)
    Sacci, John B.
49. T-Cell Mediated Inhibition of Liver-Stage Development Assay (pp. 521-528)
    Renia, Laurent; Belnoue, Elodie; Vigario, Ana Margarida
50. Antibody-Dependent Cellular Inhibition Assay (pp. 529-534)
    Druilhe, Pierre; Bouharoun-Tayoun, Hasnaa
51. Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA (pp. 535-554)
    Haynes, J. David; Moch, J. Kathleen; Smoot, Douglas S.
52. Analysis of CSA-Binding Parasites and Antiadhesion Antibodies (pp. 555-560)
    Fried, Michal; Duffy, Patrick E.
53. Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow (pp. 561-570)
    Cooke, Brian M.; Coppel, Ross L.; Nash, Gerard B.
54. Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays (pp. 571-580)
    Cooke, Brian M.; Coppel, Ross L.; Nash, Gerard B.
55. Triton X-114 Phase Partitioning for Antigen Characterization (pp. 581-586)
    Wang, Lina; Coppel, Ross L.
56. Immunoprecipitation for Antigen Localization (pp. 587-604)
    Menting, John G. T.; Coppel, Ross L.
57. Field Trials (pp. 607-615)
    Alonso, Pedro L.; Aponte, John J.

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