Library Science

  Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.

Authors: Federico, Maurizio

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Table of Contents

1. From Lentiviruses to Lentivirus Vectors (pp. 3-16)
   Federico, Maurizio
2. The Choice of a Suitable Lentivirus Vector Transcriptional Targeting (pp. 17-28)
   Lotti, Francesco; Mavilio, Fulvio
3. Choice and Use of Appropriate Packaging Cell Types (pp. 29-42)
   Pacchia, Annmarie L.; Mukherjee, Sayandip; Dougherty, Joseph P.
4. Small- to Large-Scale Production of Lentivirus Vectors (pp. 43-56)
   Marino, Michael P.; Luce, Milson J.; Reiser, Jakob
5. Detection and Titration of Lentivirus Vector Preparations (pp. 57-68)
   Gatlin, Joel; Islas-Ohlmayer, Miguel; Garcia, J. Victor
6. Detection and Selection of Lentiviral Vector-Transduced Cells (pp. 69-86)
   Cui, Yan; Chang, Lung-Ji
7. Detection of Replication-Competent Lentiviral Particles (pp. 87-94)
   Segall, Harry; Sutton, Richard E.
8. Lymphocytes (pp. 95-106)
   Chinnasamy, Dhanalakashmi; Candotti, Fabio
9. Monocyte/Macrophages and Dendritic Cells (pp. 107-116)
   Segall, Harry; Sutton, Richard E.
10. Hematopoietic Stem and Progenitor Cells (pp. 117-130)
    Donahue, Robert E.; Chen, Irvin S. Y.
11. Mesenchymal Stem Cells (pp. 131-140)
    Zhang, Xian-Yang; Russa, Vincent F. La; Reiser, Jakob
12. Hippocampal Neurons (pp. 141-154)
    Nadeau, Jay L.
13. Dorsal Root Ganglia Sensory Neurons (pp. 155-168)
    Fleming, Jane; Ginn, Samantha L.; Alexander, Ian E.
14. Cardiomyocytes (pp. 169-180)
    Bonci, Desiree; Latronico, Michael V. G.; Condorelli, Gianluigi
15. Airway Epithelia (pp. 181-196)
    Johnson, Larry G.; Randell, Scott H.; Olsen, John C.
16. Corneal Cells (pp. 197-204)
    Appukuttan, Binoy; McFarland, Trevor; Stout, J. Timothy
17. Retinal Tissue (pp. 205-216)
    McFarland, Trevor; Appukuttan, Binoy; Stout, J. Timothy
18. HIV-2 Vectors (pp. 217-232)
    Arya, Suresh K.; Cho, Jean E.; Chang, Suzie H.
19. SIV Vectors (pp. 233-250)
    Ruggieri, Alessia; Negre, Didier; Cosset, Francois-Loic
20. FIV Vectors (pp. 251-272)
    Loewen, Nils; Barraza, Roman; Whitwam, Todd; Saenz, Dyana T.; Kemler, Iris; Poeschla, Eric M.
21. Hybrid Lentivirus Vectors (pp. 273-284)
    Planelles, Vicente
22. Cells of Respiratory Epithelium (pp. 285-298)
    Sinn, Patrick L.; McCray, Paul B.
23. Cells of the Nervous System (pp. 299-308)
    Rosenblad, Carl; Lundberg, Cecilia

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