9th
December
2006
Lectin Methods and Protocols
posted in BioLibrary, Library |
A comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are detailed methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology.
Ed. Rhodes, Jonathan M. ; Milton, Jeremy D.
Table of Contents
- Light Microscopy Overview and Basic Methods (pp. 3-20)
Leathem, Anthony J.; Brooks, Susan A. - Lectin Histochemistry and Cytochemistry-Light Microscopy: Avidin-Biotin Amplification on Resin- Embedded Sectlons (pp. 21-40)
Stoddart, Robert W.; Jones, Carolyn J. P. - Lectin-Gold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins (pp. 41-54)
Roth, Jiirgen; Zuber, Christian; Sata, Tetsutaro; Li, Wei-Ping - Use of Fluorochrome-Labeled Lectins in Light Microscopy (pp. 55-64)
Schumacher, Udo; Mitchell, Barry S. - The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage (pp. 65-72)
Hoedt-Schmidt, Sibylle - Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis (pp. 73-94)
Helliwell, Timothy Richard - Electron Microscopy: Use of Lectin-Peroxidase Prior to Embedding (pp. 97-110)
Ellinger, Adi - Electron Microscopy: Use of Lectin-Gold After Embedding (pp. 111-120)
Herken, Rainer; Manshausen, Berti - Amplification of Lectin-Gold Histochemistry (pp. 121-132)
Madrld, Juan F.; Hernandez, Francisco; Ballesta, Jose - Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines (pp. 133-143)
Mitchell, Barry S.; Schumacher, Udo - Application of Sequential Smith Degradation to Lectin Blots (pp. 147-157)
Ching, Chi Kong - Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues (pp. 159-166)
Zuber, Christian; Li, Wei-Ping; Roth, Jurgen - Characterization of HIV gpl20 Envelope Glycoprotein by Lectin Analysis (pp. 167-174)
Gram, Gregers J.; Hansen, John-Erik Stig - Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins (pp. 175-192)
Olofsson, Sigvard; Bolmstedt, Anders - Use of Lectin for Detection of Agalactosyl IgG (pp. 195-206)
Tsuchiya, Naoyuki; Endo, Tamao; Kochibe, Naohisa; Ito, Koji; Kobata, Akira - Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins: a-1-Antitrypsin (pp. 207-214)
Aoyagi, Yutaka; Asakura, Hitoshi - Detection of Altered Glycosylation of a-Fetoprotein Using Lectin-Affinity Electrophoresis (pp. 215-226)
Taketa, Kazuhisa; Liu, Miao; Taga, Hiroko - Use of Lectin-Affinity Electrophoresis for Quantification and Characterization of Glycoforms of a-1 Acid Glycoprotein (pp. 227-234)
Bag-Hansen, Thorkild C. - ABO(H) Blood Group Expression on Circulating Glycoproteins (pp. 235-245)
Matsui, Taei; Titani, Koiti - Lectin/Antibody ?Sandwich? ELISA for Quantification of Circulating Mucin as a Diagnostic Test for Pancreatic Cancer (pp. 249-254)
Parker, Neil - Quantification of Intestinal Mucins (pp. 255-261)
Milton, Jeremy D.; Rhodes, Jonathan M. - Purification and Characterization of Human Serum and Secretory IgAl and lgA2 Using Jacalin (pp. 265-278)
Kerr, Michael A.; Loomes, Lesley M.; Bonner, Brian C.; Hutchings, Amy B.; Senior, Bernard W. - Use of Lectins in Affinity Purification of HIV and SW Envelope Glycoproteins (pp. 279-290)
Gilljam, Gustav - T-Cell Receptor Purification (pp. 291-298)
Kearse, Kelly P. - Use of Monomeric, Monovalent Lectin Derivatives for Flow Cytometric Analysis of Cell Surface Glycoconjugates (pp. 301-306)
Kaku, Hanae; Shibuya, Naoto - Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry (pp. 307-315)
Guasch, Rosa M.; O?Connor, Jose-Enrique - Lectins as Tools for the Purification of Liver Endothelial Cells (pp. 319-328)
Gomez, Daniel E.; Thorgeirsson, Unnur P. - The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation (pp. 329-350)
Nagler, Arnon; Morecki, Shoshana; Slavin, Shimon - Combined LectinlMonoclonal Antibody Purging of Bone Marrow for Use in Conjunction with Autologous Bone Marrow Transplantation in the Treatment of Multiple Myeloma (pp. 351-361)
Rhodes, Elizabeth G. H. - Mechanisms and Assessment of Mitogenesis An Overview (pp. 365-378)
Kilpatrick, David C. - Mitogenic Effects of Lectins on Epithelial Cells (pp. 379-384)
Yu, Lu-Gang; Rhodes, Jonathan M. - Use of Lectins as Mitogens for Lymphocytes (pp. 385-392)
Kilpatrick, David C. - Effect of Lectins on Uptake of Polyamines (pp. 393-406)
Bardocz, Susan; White, Ann - Effects of Lectins on Cytoskeletal Organization in Mammalian Cells (pp. 407-422)
Carinci, Paolo; Becchetti, Ennio; Bodo, Maria - Effect of Lectins on Protein Kinase Activity (pp. 423-432)
Sada, Kiyonao; Yamamura, Hirohei - Lectin-Induced Calcium Mobilization in Human Platelets: Use of Fluorescent Probes (pp. 433-440)
Ramaschi, Giuseppe; Torti, Mauro - Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells (pp. 441-452)
Timoshenko, Alexander V.; Kayser, Klaus; Gabius, Hans-Joachim - Cytotoxic Effects of Lectins (pp. 453-460)
Gorelik, Elieser - Application of the Lectin-Dependent Cell-Mediated Cytotoxicity Assay to Bronchoalveolar Lavage Fluid and Venous Blood Samples Collected from Canine Lung Allografts (pp. 461-472)
Lee, Allan G. L.; Shennib, Hani A. - The Effect of Lectins on Crypt Cell Proliferation in Organ Culture (pp. 475-482)
Ryder, Stephen D. - Effects of Lectin Ingestion on Animal Growth and Internal Organs (pp. 485-494)
Pusztai, Arpad - Lectin Ingestion: Changes in Mucin Secretion and Bacterial Adhesion to Intestinal Tissue (pp. 495-504)
Ceri, Howard; Banwell, John G.; Fang, Rixun - Assessment of Lectin Inactivation by Heat and Digestion (pp. 505-514)
Pusztai, Arpad; Grant, George - Use of Lectins as Transganglionic Neuronal Tracers in the Study of Unmyelinated Primary Sensory Neurons (pp. 517-526)
Plenderleith, Mark B.; Snow, Peter J. - Lectin Inhibition of Bacterial Adhesion to Animal Cells (pp. 529-538)
Stinson, Murray W.; Wang, Jen Ren - Inhibition of HIV Infection by Lectin Binding to CD4 (pp. 539-554)
Favero, Jean; Lafont, Virginie - Inhibition of HIV Infection by Lectin Binding to gp120 (pp. 555-564)
Animashaun, Theresa; Mahmood, Naheed - Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis (pp. 567-582)
Haltner, Ellen; Borchard, Gerrit; Lehr, Claus-Michael - The Use of Lectins for Liposome Targeting in Drug Delivery (pp. 583-594)
Kaszuba, Michael; Jones, Malcolm N.