Library Science

Luke O’Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave’s disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

Authors: O’Neill, Luke A. J. ; Bowie, Andrew

Table of Contents

1. ELISAs and Interleukin Research (pp. 003-015)
   Greene, Catherine
2. ELISPOT Technique for Assaying Interleukins (pp. 017-028)
   Hauer, Almuth Christine; Bajaj-Elliott, Mona
3. Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay) (pp. 029-039)
   Okamoto, Yoshihiro; Nishida, Mikio
4. Assaying Interleukins Intracellularly by FACS (pp. 041-048)
   O???Mahony, Liam; O???Donoghue, Maurice
5. Basic RT-PCR for Measurement of Cytokine Expression (pp. 049-058)
   Bajaj-Elliott, Mona; Hauer, Almuth Christine
6. Quantitative PCR for Measurement of Cytokine Expression (pp. 059-076)
   Nilsen, Ellen M; Johansen, Finn-Eirik; Brandtzaeg, Per
7. Single Cell RT-PCR for the Detection of Cytokine mRNA (pp. 077-081)
   Ligumsky, Moshe; Nechushtan, Hovav; Razin, Ehud
8. Quantitation of Cytokine mRNA by Flash-Type Bioluminescence (pp. 083-097)
   Actor, Jeffrey K
9. Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method (pp. 099-109)
   Tardif, Ginette; Di Battista, John A; Pelletier, Jean-Pierre; Martel-Pelletier, Johanne
10. Quantification of Mouse IL-6 and TNF-a mRNA Using Xplore Assays (pp. 111-124)
    Van Arsdell, Scott W; Murphy, Kevin P; Pazmany, Csaba; Erickson, Diane; Moody, Mark D
11. Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay (pp. 125-143)
    Wang, Zheng-Ming; Dziarski, Roman
12. Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography (pp. 145-155)
    Phillips, Terry M; Dickens, Benjamin F
13. Detection of Cytokines by Immunohistochemistry (pp. 157-162)
    Huleihel, Mahmoud
14. Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement (pp. 163-174)
    Petrovsky, Nikolai
15. Assaying Interleukin-8 (pp. 177-183)
    Moynagh, Paul N
16. Measurement of Interleukin-2 by Bioassay (pp. 185-192)
    Weston, Lyanne
17. Assaying Interleukin-15 (pp. 193-204)
    Douvdevani, Amos; Weiler, Michal; Lewis, Eli
18. Assaying Interleukin-6 in Breast Cancer (pp. 207-216)
    Harmey, Judith; Haverty, Amanda; Foley, Deirdre; Bouchier-Hayes, David
19. Cytokines, Stress, and Depressive Illness (pp. 217-238)
    Anisman, Hymie; Hayley, Shawn; Ravindran, Arun V; Merali, Zul; Griffiths, Jenna
20. Measurement of Interleukins in Cutaneous Disorders: Competitive RT-PCR for Determination of IL-10 mRNA Expression in Psoriasis (pp. 239-250)
    Asadullah, Khusru; Haeussler-Quade, Antje; Doecke, Wolf Dietrich; Sterry, Wolfram; Volk, Hans-Dieter
21. Interleukins in Graves??? Disease (pp. 251-261)
    Komorowski, Jan
22. Evaluation of Cytokines in Migraine Patients by ELISA (pp. 263-272)
    Munno, Irene; Marinaro, Mariarosaria; Lacedra, Giuseppe; Bassi, Antonia; Centonze, Vincenzo
23. Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction (pp. 273-281)
    Pudil, Radek
24. Measurement of Cytokines in Peritoneal Fluids (pp. 285-291)
    Punnonen, Reijo; Punnonen, Juha
25. Measurement of Cytokines in Seminal Plasma (pp. 293-304)
    Glezerman, Marek; Lunenfeld, Eitan; Huleihel, Mahmoud
26. Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD (pp. 305-312)
    Gibson, Peter G; Simpson, Jodie L
27. Cytokines in Blister Fluids (pp. 313-325)
    Ameglio, Franco; D???Auria, Luciano
28. Measurement of Cytokines in Synovial Fluid (pp. 327-335)
    De Benedetti, Fabrizio
29. Measurement of Cytokines in Cerebrospinal Fluid (pp. 337-344)
    Yokoyama, Toshiyuki
30. Detection of Cytokines in Tears (pp. 345-353)
    Thakur, Archana; Willcox, Mark D P
31. Measurement of Phosphoinositide 3-Kinase Activation by Interleukins (pp. 357-368)
    Ward, Stephen G
32. Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis (pp. 369-383)
    Brennan, Paul; Athie-Morales, Veronica
33. Applications for Green Fluorescent Protein in Cell Signaling (pp. 385-391)
    Brennan, Paul; Astoul, Emmanuelle
34. Use of Pichia pastoris for Production of Recombinant Cytokines (pp. 393-407)
    Murphy, Kevin P; Pazmany, Csaba; Moody, Mark D
35. Analysis of Single Nucleotide Polymorphisms in the Interleukin-1B Gene Using 5??? Nuclease Assays (pp. 409-417)
    El-Omar, Emad M
36. Measurement of Cytokine and Chemokine Gene Expression Patterns Using cDNA Array (pp. 419-438)
    Wang, Zheng-Ming; Dziarski, Roman
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