Library Science

This book provides molecular biology laboratories with the most powerful techniques for employing in vitro transcription and translation systems. Detailed experimental protocols are provided for prokaryotic transcription and translation systems, along with protocols for the many techniques used in the analysis of eukaryotic transcription. The collection is unique in that it also contains protocols for core techniques that use the products of in vitro transcription and translation systems, such as protein import into mitochondria and chloroplasts and structure???function studies. Also covered are core techniques in the analysis of promoters and transcription factors.

Authors: Tymms, Martin J.

Download Book (PDF 30224K)

Table of Contents

1. Transcription In Vitro Using Bacteriophage RNA Polymerases (pp. 1-12)
   Schenborn, Elaine T.
2. Subtraction Hybridization cDNA Libraries (pp. 13-30)
   Schweinfest, Clifford W.; Nelson, Peter S.; Graber, Michael W.; Demopoulos, Rita I.; Papas, Takis S.
3. Quantitative Measurement of mRNA Using the RNase Protection Assay (pp. 31-46)
   Tymms, Martin J.
4. Assembly and Transcription of Chromatin Templates Using RNA Polymerase III (pp. 47-66)
   Felts, Sara J.
5. Antisense Affinity Depletion of RNP Particles: Application to Spliceosomal snRNPs (pp. 67-76)
   Blencowe, Benjamin J.; Barabino, Silvia M. L.
6. In Vitro mRNA Editing Using S100 Extracts (pp. 77-88)
   Greeve, Jobst; Scott, James
7. An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA (pp. 89-106)
   Phillips, Don R.; Crothers, Donald M.
8. In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Breast Carcinoma Cells (pp. 107-120)
   Bagchi, Milan K.; Tsai, Sophia Y.; Tsai, Ming-Jer
9. Plastid In Vitro Transcription (pp. 121-134)
   Tiller, Kai; Link, Gerhard
10. Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Early Embryos to Study the Gain- and Loss-of-Function of Genes (pp. 135-150)
    Kola, Ismail; Sumarsono, Sony Heru
11. mRNA Translation in Xenopus Oocytes (pp. 151-178)
    Ceriotti, Aldo; Colman, Alan
12. Transcription System Using a HeLa Cell Mitochondrial Lysate (pp. 179-198)
    Kruse, Brigitte; Murdter, Nalini N.; Attardi, Giuseppe
13. The Xenopus Egg Extract Translation System (pp. 199-214)
    Matthews, Glenn M.; Colman, Alan
14. In Vitro Translation Using Rabbit Reticulocyte Lysate (pp. 215-232)
    Beckler, Gregory S.; Thompson, David; Van Oosbree, Tom
15. An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori (pp. 233-244)
    Sakurai, Hiroshi; Izumi, Susumu; Tomino, Shiro
16. Translation Using a Wheat-Germ Extract (pp. 245-252)
    Van Herwynen, John F.; Beckler, Gregory S.
17. In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani (pp. 253-264)
    Andersen-Beckh, Bettina; Niemann, Heiner
18. Preparation and Use of E. coli S-30 Extracts (pp. 265-278)
    Lesley, Scott G.
19. Posttranslational Transport and In Thylakoido Integration of Plastid Protein (pp. 279-292)
    Kruse, Elisabeth; Kloppstech, Klaus
20. Import into Isolated Yeast Mitochondria of Radiolabeled Proteins Synthesized In Vitro (pp. 293-316)
    Law, Ruby H. P.; Nagley, Phillip
21. Structure???Function Studies Based on In Vitro Expression (pp. 317-336)
    Tymms, Martin J.; Hertzog, Paul J.
22. ??-Like Plastid Transcription Factors (pp. 337-348)
    Tiller, Kai; Link, Gerhard
23. Preparation of HeLa Nuclear Extracts (pp. 349-358)
    Pugh, B. Franklin
24. Purification of the Human TATA-Binding Protein, TBP (pp. 359-368)
    Pugh, B. Franklin
25. DNA-Binding Studies Using In Vitro Synthesized Myb Proteins (pp. 369-378)
    Ramsay, Robert George
26. DNase I Footprinting Using PCR-Generated End-Labeled DNA Probes (pp. 379-392)
    Iannello, Rocco C.
27. Gene Isolation by Screening (lambda)gt11 Expression Libraries with DNA-Binding Site Probes (pp. 393-408)
    Kalionis, Bill
28. Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay (pp. 409-422)
    Hodge, David R.; Thompson, Delores M.; Panayiotakis, Alexandra; Seth, Arun

This entry was posted on Wednesday, February 7th, 2007 at 2:35 pm and is filed under BioLibrary, Molecular Biology. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.