Library Science

In Vitro Toxicity Testing Protocols is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book’s expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable. Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step protocols are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments.

Authors: O’Hare, Sheila; Atterwill, Christopher K.

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Table of Contents

1. Alternative Method of Assessing Toxicity (pp. 1-10)
   Atterwill, Chris K
2. LLC-RK1 Cell Screening Test for Nephrotoxicity (pp. 11-16)
   White, David J.; Seaman, Chris
3. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity (pp. 17-24)
   Cookson, Mark R.; McClean, R.; Pentreath, Victor W.
4. Human Thyroid Culture (pp. 25-32)
   Mothersill, Carmel
5. Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity Testing (pp. 33-42)
   Bidey, Stephen P.
6. Dust Toxicity in Rat Alveolar Macrophage Cultures (pp. 43-50)
   Collan, Yrjo; Kosma, Veli-Matti
7. Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity (pp. 51-58)
   Ferro, Margherita
8. Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytes (pp. 59-66)
   Brambilla, Giovanni; Martelli, Antonietta
9. Model Cavity Method (pp. 67-74)
   INVITTOX
10. Human Esophageal Culture (pp. 75-80)
    Mothersill, Carmel
11. The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing (pp. 81-94)
    Gillies, Glenda E.; Buckingham, Julia C.
12. The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing (pp. 95-108)
    Gillies, Glenda E.; Buckingham, Julia C.
13. The FRAME Cytotoxicity Test (Kenacid Blue) (pp. 109-118)
    Clothier, Richard H.
14. Allium Test (pp. 119-128)
    Fiskesjo, Geirid
15. Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose (pp. 129-136)
    Walum, Erik; Forsby, Anna
16. MTT Assays (pp. 137-150)
    Supino, Rosa
17. V79 Cytotoxicity Test for Membrane Damage (pp. 151-160)
    Bianchi, Vera
18. SIRC Cytotoxicity Test (pp. 161-168)
    Blein-Sella, Odile; Adolphe, Monique
19. Rabbit Articular Chondrocyte Functional Toxicity Test (pp. 169-176)
    Blein-Sella, Odile; Adolphe, Monique
20. Balb/c 3T3 Cytotoxicity Test (pp. 177-188)
    Liebsch, H. M.; Spielmann, Horst
21. The Pollen Tube Growth Test (pp. 189-198)
    Kristen, Udo; Kappler, Rolf
22. HET-CAM Test (pp. 199-204)
    Spielmann, Horst
23. The Use of the Bovine Isolated Cornea as a Possible In Vitro Test for Ocular Irritancy (pp. 205-210)
    Northover, Ann M.
24. Cytotoxicity in an Anchorage-Independent Fibroblast Cell Line Measured by a Combination of Fluorescent Dyes (pp. 211-218)
    Kemp, Richard B.
25. Cell Culture Phototoxicity Test (pp. 219-226)
    Duffy, Paul A.
26. The Murine Local Lymph Node Assay (pp. 227-236)
    Hilton, Jennifer; Kimber, Ian
27. Polymorphonuclear Leukocyte Locomotion (pp. 237-244)
    Valentino, Matteo
28. Immune Function Assays (pp. 245-256)
    Nicklin, Steve
29. The DNA Alkaline Unwinding Genotoxicity Test (pp. 257-266)
    Bolcsfoldi, Georg
30. Measurement of Unscheduled DNA Synthesis In Vitro Using Primary Rat Hepatocyte Cultures (pp. 267-276)
    Dean, Steve
31. Gene Mutation Assays in Mammalian Cells (pp. 277-286)
    Clements, Julie
32. Measurement of Chromosome Aberrations In Vitro Using Human Peripheral Blood Lymphocytes (pp. 287-296)
    Marshall, Richard
33. Mutation Assays in Bacteria (pp. 297-306)
    Clare, Christopher
34. Chick Embryotoxicity Screening Test (CHEST I and II) (pp. 307-310)
    Davies, Wendy J.; Freeman, Stuart J.
35. Frog Embryo Teratogenesis Assay: Xenopus (FETAX) (pp. 311-316)
    Davies, Wendy J.; Freeman, Stuart J.
36. The Drosophila melanogaster Assay (pp. 317-320)
    Davies, Wendy J.; Freeman, Stuart J.
37. The Hydra attenuata Assay (pp. 321-326)
    Davies, Wendy J.; Freeman, Stuart J.

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