Library Science

in-vitro-transcription-and-translation-protocols.pdf 

Authors: Trower, Michael K.

Table of Contents

• Site-Directed Mutagenesis Using Positive Antibiotic Selection (pp. 1-12)
  Bohnsack, Richard N.
• In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method (pp. 13-30)
  Zhu, Li
• Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates (pp. 31-44)
  Braman, Jeffrey; Papworth, Carol; Greener, Alan
• Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template (pp. 45-54)
  Hagemeier, Christian
• Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach (pp. 55-64)
  Dale, Susan J.; Felix, Ian R.
• Analysis of Point Mutations by Use of Amber Stop Codon Suppression (pp. 65-74)
  Lesley, Scott A.
• A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA (pp. 75-86)
  Lai, Derhsing; Pestka, Sidney
• Double-Stranded DNA Site-Directed Mutagenesis (pp. 87-96)
  Viville, Stephane
• Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template (pp. 97-108)
  Edward, Roy
• Targeted Mutagenesis Mediated by the Triple Helix Formation (pp. 109-118)
  Glazer, Peter M.; Wang, Gan; Havre, Pamela A.; Gunther, Edward J.
• A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site (pp. 119-138)
  Zhu, Li; Holtz, Ann E.
• Ordered Deletions Using Exonuclease III (pp. 139-148)
  Clark, Denise; Henikoff, Steven
• Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis (pp. 149-156)
  Rouwendal, Gerard J. A.; Wolbert, Emil J. H.; Zwiers, Lute-Harm; Springer, Jan
• PCR-Based Site-Directed Mutagenesis (pp. 157-166)
  Shimada, Atsushi
• In Vitro Recombination and Mutagenesis by Overlap Extension PCR (pp. 167-176)
  Pogulis, Robert J.; Vallejo, Abbe N.; Pease, Larry R.
• Site-Directed Mutagenesis Using Overlap Extension PCR (pp. 177-192)
  Aiyar, Ashok; Xiang, Yan; Leis, Jonathan
• Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template (pp. 193-202)
  Mikaelian, Ivan; Sergeant, Alain
• Site-Directed Mutagenesis In Vitro by Megaprimer PCR (pp. 203-216)
  Barik, Sailen
• Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids (pp. 217-228)
  Watkins, Brynmor A.; Reitz, Marvin S.
• PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments (pp. 229-238)
  Tessier, Daniel C.; Thomas, David Y.
• Site-Directed Mutagenesis Using a Rapid PCR-Based Method (pp. 239-248)
  Costa, Gina L.; Bauer, John C.; McGowan, Barbara; Angert, Mila; Weiner, Michael P.
• A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence (pp. 249-258)
  Tomic-Canic, Marjana; Bernerd, Francoise; Blumenberg, Miroslav
• A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered (pp. 259-268)
  Tomic-Canic, Marjana; Sunjevaric, Ivana; Blumenberg, Miroslav
• Multiple Site-Directed Mutagenesis (pp. 269-278)
  Bhat, Kolari S.
• Construction of Linker-Scanning Mutations by Oligonucleotide Ligation (pp. 279-286)
  Hobson, Grace M.; Harlow, Patricia P.; Benfield, Pamela A.
• Construction of Linker-Scanning Mutations Using PCR (pp. 287-296)
  Harlow, Patricia P.; Hobson, Grace M.; Benfield, Pamela A.
• Use of Codon Cassette Mutagenesis for Saturation Mutagenesis (pp. 297-310)
  Kegler-Ebo, Deena M.; Polack, Glenda W.; DiMaio, Daniel
• Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) (pp. 311-322)
  Chiang, Lillian W.
• Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides (pp. 323-334)
  Kirchhof, Frank; Desrosiers, Ronald C.
• Random Sequence Mutagenesis for the Generation of Active Enzymes (pp. 335-350)
  Black, Margaret E.; Loeb, Lawrence A.
• Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR (pp. 351-356)
  Kuipers, Oscar P.
• PCR-Mediated Chemical Mutagenesis (pp. 357-368)
  Roufa, Donald J.
• Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method (pp. 369-374)
  Dale, Susan J.; Belfield, Maxine
• An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain (pp. 375-386)
  Greener, Alan; Callahan, Marie; Jerpseth, Bruce

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