Library Science

Lorette Javois’ timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: “???a useful, sometimes beautiful compilation of protocols and methods???” - FEBS Letters “???an extremely useful ???vade mecum’ of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use.” - Society for General Microbiology Quarterly

Authors: Javois, Lorette C.

Download Book (PDF 25078K)

Table of Contents

1. Overview of Antibody Use in Immunocytochemistry (pp. 3-12)
   Mao, Su-Yau; Javois, Lorette C.; Kent, Ute M.
2. Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration (pp. 13-22)
   Kent, Ute M.
3. Purification of Antibodies Using Ion-Exchange Chromatography (pp. 23-28)
   Kent, Ute M.
4. Purification of Antibodies Using Affinity Chromatography (pp. 29-36)
   Kent, Ute M.
5. Purification of Antibodies Using Protein A-Sepharose and FPLC (pp. 37-42)
   Kent, Ute M.
6. Conjugation of Fluorochromes to Antibodies (pp. 43-48)
   Mao, Su-Yau
7. Biotinylation of Antibodies (pp. 49-52)
   Mao, Su-Yau
8. Overview of Cell Fixation and Permeabilization (pp. 55-66)
   Melan, Melissa A.
9. Preparation of Frozen Sections for Analysis (pp. 67-74)
   Bratthauer, Gary L.
10. Processing of Cell-Touch Preparations (pp. 75-80)
    Bratthauer, Gary L.
11. Processing of Cytological Specimens (pp. 81-88)
    Bratthauer, Gary L.
12. Processing of Tissue-Culture Cells (pp. 89-96)
    Bratthauer, Gary L.
13. Processing of Tissue Specimens (pp. 97-104)
    Bratthauer, Gary L.
14. Overview of Fluorophores (pp. 107-116)
    Mullins, J. Michael
15. Direct Immunofluorescent Labeling of Cells (pp. 117-122)
    Javois, Lorette C.
16. Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells (pp. 123-130)
    Willingham, Mark C.
17. Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells (pp. 131-142)
    Willingham, Mark C.
18. Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts (pp. 143-154)
    Javois, Lorette C.
19. Overview of Antigen Detection Through Enzymatic Activity (pp. 155-164)
    Bratthauer, Gary L.
20. The Peroxidase???Antiperoxidase (PAP) Method (pp. 165-174)
    Bratthauer, Gary L.
21. The Avidin???Biotin Complex (ABC) Method (pp. 175-184)
    Bratthauer, Gary L.
22. Labeled Avidin Binding (LAB) Method (pp. 185-194)
    Bratthauer, Gary L.
23. Avidin???Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue (pp. 195-204)
    Raffeld, Mark; Jaffe, Elaine S.
24. ELISA on Attachment-Dependent or Suspension Grown Cells (pp. 205-210)
    Brent, Julie Ann
25. Use of Immunogold with Silver Enhancement (pp. 211-216)
    Oliver, Constance
26. Overview of Flow Cytometry and Fluorescent Probes for Cytometry (pp. 219-224)
    Cunningham, Robert E.
27. Tissue Disaggregation (pp. 225-228)
    Cunningham, Robert E.
28. Indirect Immunofluorescent Labeling of Viable Cells (pp. 229-232)
    Cunningham, Robert E.
29. Indirect Immunofluorescent Labeling of Fixed Cells (pp. 233-238)
    Cunningham, Robert E.
30. Fluorescent Labeling of DNA (pp. 239-242)
    Cunningham, Robert E.
31. Deparaffinization and Processing of Pathologic Material (pp. 243-248)
    Cunningham, Robert E.
32. Assay for Phagocytosis (pp. 249-260)
    Harvath, Liana; Terle, Douglas A.
33. Assay for Filamentous Actin (pp. 261-268)
    Harvath, Liana
34. Assay for Chemoattractant Binding (pp. 269-280)
    Harvath, Liana; Aksamit, Robert R.; Cunningham, Robert E.
35. Assay for Oxidative Metabolism (pp. 281-288)
    Harvath, Liana; Terle, Douglas A.
36. Fixation and Embedding (pp. 291-298)
    Oliver, Constance
37. Preparation of Colloidal Gold (pp. 299-302)
    Oliver, Constance
38. Conjugation of Colloidal Gold to Proteins (pp. 303-308)
    Oliver, Constance
39. Colloidal Gold/Streptavidin Methods (pp. 309-314)
    Oliver, Constance
40. Pre-Embedding Labeling Methods (pp. 315-320)
    Oliver, Constance
41. Postembedding Labeling Methods (pp. 321-328)
    Oliver, Constance
42. Overview of Fluorescence Photomicrography (pp. 331-334)
    Javois, Lorette C.; Mullins, J. Michael
43. Overview of Fluorescence Analysis with the Confocal Microscope (pp. 337-348)
    Harvath, Liana
44. Overview of Laser Microbeam Applications as Related to Antibody Targeting (pp. 349-366)
    Pine, P. Scott
45. Nucleic Acid Immunocytochemistry: In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe (pp. 367-374)
    Nardone, Roland M.
46. Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes (pp. 375-382)
    Nardone, Roland M.
47. Overview of Automated Immunostainers (pp. 383-404)
    Herman, Gilbert E.; Elfont, Edna A.; Floyd, Alton D.
48. Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors: Diagnostic Applications in the Clinical Laboratory (pp. 405-428)
    Frisman, Dennis M.

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