23rd
January
2007
Immunochemical Protocols
posted in BioLibrary, Molecular Biology |Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.
Authors: Manson, Margaret M.
Table of Contents
- Production of Polyclonal Antisera (pp. 1-6)
Green, Jonathan A.; Manson, Margaret M. - Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (Immobilon) After Transfer from SDS-PAGE and Their Use as Immunogens (pp. 7-12)
Szewczyk, Boguslaw; Summers, Donald F. - Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen (pp. 13-22)
Diano, Monique; Le Bivic, Andre; Hirn, Michel - Synthesis of Peptides for Use as Immunogens (pp. 23-32)
Hancock, David C.; Evan, Gerard I. - Production and Characterization of Antibodies Against Synthetic Peptides (pp. 33-42)
Hancock, David C.; Evan, Gerard I. - Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins (pp. 43-64)
Dean, Christopher J. - Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase (pp. 65-78)
Frobert, Yveline; Grassi, Jacques - Purification of Immunoglobulin G (IgG) (pp. 79-104)
Baines, Michael G.; Thorpe, Robin - Epitope Mapping (pp. 105-116)
Mole, Sara E. - Enzyme???Antienzyme Method for Immunohistochemistry (pp. 117-124)
Ormerod, Michael G.; Imrie, Susanne E - Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates (pp. 125-130)
Green, Jonathan A.; Manson, Margaret M. - Immunohistochemical Detection of Biomodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies (pp. 131-136)
Green, Jonathan A.; Edwards, Richard E.; Manson, Margaret M. - Avidin???Biotin Technology: Preparation of Biotinylated Probes (pp. 137-142)
Bayer, Edward A.; Wilchek, Meir - Avidin???Biotin Technology: Preparation of Avidin Conjugates (pp. 143-148)
Bayer, Edward A.; Wilchek, Meir - Immunochemical Applications of Avidin???Biotin Technology (pp. 149-162)
Bayer, Edward A.; Wilchek, Meir - Preparation of Gold Probes (pp. 163-168)
Beesley, Julian E. - Immunogold Probes for Light Microscopy (pp. 169-176)
Beesley, Julian E. - Immunogold Probes in Electron Microscopy (pp. 177-186)
Beesley, Julian E. - Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species (pp. 187-194)
Bienz, Kurt; Egger, Denise - Quantitative and Qualitative Immunoelectrophoresis: General Comments on Principles, Reagents, Equipment, and Procedures (pp. 195-200)
Laine, Anne - Rocket Immunoelectrophoresis Technique or Electroimmunodiffusion (pp. 201-206)
Laine, Anne - Crossed Immunoelectrophoresis (pp. 207-214)
Laine, Anne - Crossed Immunoaffinoelectrophoresis (pp. 215-220)
Laine, Ann - Immunodetection of Proteins by Western Blotting (pp. 221-234)
Henderson, Colin J.; Wolf, C. Roland - Erasable Western Blots (pp. 235-246)
Kaufmann, Scott H.; Shaper, Joel H. - Colloidal Gold Staining and Immunoprobing on the Same Western Blot (pp. 247-254)
Egger, Denise; Bienz, Kurt - Colloidal Gold Staining and Immunodetection in 2-D Protein Mapping (pp. 255-260)
Schapira, Anthony H. K - Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen (pp. 261-266)
Szewczyk, Boguslaw; Summers, Donald F. - Competitive ELISA (pp. 267-272)
Makarananda, Kitti; Neal, Gordon E. - Twin-Site ELlSAs for fos and myc Oncoproteins Using the AMPAK System (pp. 273-282)
Moore, John P.; Bates, David L. - Preparation of Cytotoxic Antibody???Toxin Conjugates (pp. 283-294)
Cumber, Alan J.; Wawrzynczak, Edward J. - Immunoaffinity Purification and Quantification of Antibody???Toxin Conjugates (pp. 295-306)
Wawrzynczak, Edward J.; Cumber, Alan J. - An Immuno-Slot-Blot Assay for Detection and Quantitation of Alkyldeoxyguanosines in DNA (pp. 307-320)
Ludeke, Barbara I. - Production of Monoclonal Antibodies for the Detection of Chemically Modified DNA (pp. 321-328)
Tilby, Michael J. - Sensitive Competitive Enzyme-Linked Immunoassay for Quantitation of Modified Bases in DNA (pp. 329-336)
Tilby, Michael J. - An Immunochemical Assay for Detecting Transition of B-DNA to Z-DNA (pp. 337-346)
Thomas, T. J. - Cell Sorting Using Immunomagnetic Beads (pp. 347-358)
Wang, Eddie C. Y.; Borysiewicz, Leszek K.; Weetman, Anthony P. - Cell Preparation for Flow Cytometry (pp. 359-362)
Ormerod, Michael G. - Preparation of Rat Lung Cells for Flow Cytometry (pp. 363-368)
Martin, Janet; White, Ian N. H. - The Isolation of Rat Hepatocytes for Flow Cytometry (pp. 369-372)
Davies, Reginald - Flow Cytometric Analysis of Cells Using an Antibody to a Surface Antigen (pp. 373-380)
Ormerod, Michael G. - Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry (pp. 381-386)
Ormerod, Michael G. - Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine (pp. 387-398)
Wilson, George D. - Production and Use of Nonradioactive Hybridization Probes (pp. 399-408)
Chan, Victor T.-W.; McGee, James O???D. - Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization (pp. 409-420)
Herrington, C. Simon; McGee, James O???D. - Chromosomal Mapping of Genes by Nonisotopic In Situ Hybridization (pp. 421-430)
Bhatt, Bhupendra; McGee, James O???D. - Nonisotopic In Situ Hybridization: Immunocytochemical Detection of Specific Repetitive Sequences on Chromosomes and Interphase Nuclei (pp. 431-440)
Crolla, John A. - Biotinylated Probes in Colony Hybridization (pp. 441-450)
Haas, Michael J. - Screening of (lambda)gt11 cDNA Libraries Using Monoclonal Antibodies (pp. 451-460)
Webster, Duncan F.; Melvin, William T.; Burke, M. Danny; Carr, Francis J. - Expression of Foreign Genes in Mammalian Cells Using an Antibody Fusion System (pp. 461-474)
Forster, Simon J.; Carr, Francis J.; Harris, William J.; Hamilton, Anita A.