Imaging in Biological Research, Part A
posted in Enzymology |
As these volumes were being completed, American Paul C. Lauterbur and Briton Sir Peter Mansfield won the 2003 Nobel Prize for medicine for discoveries leading to the development of MRI.
The Washington Post story on October 6, 2003 announced the accolade, noting: “Magnetic resonance imaging, or MRI, has become a routine method for medical diagnosis and treatment. It is used to examine almost all organs without need for surgery, but is especially valuable for detailed examination of the brain and spinal cord.” Unfortunately, the article overlooked the growing usefulness of this technique in basic research. MRI, along with other imaging methods, has made it possible to glance inside the living system. For patients, this may obviate the need for surgery; for researchers, it becomes a noninvasive method that enables the model systems to continue “doing what they do” without being disturbed. The value and potential of these techniques is enormous, and that is why these once clinical methods are finding their way to the laboratory.
Authors have been selected based on research contributions in the area about which they have written and based on their ability to describe their methodological contributions in a clear and reproducible way. They have been encouraged to make use of graphics and comparisons to other methods, and to provide tricks and approaches that make it possible to adapt methods to other systems.
Authors: P. Michael Conn
imaging-in-biological-research-part-a.pdf
Table of Contents
- Isolation and Identification of Yeasts from Natural Habitats (pp. 1-4)
Spencer, John F. T.; Spencer, Dorothy M. - Maintenance and Culture of Yeasts (pp. 5-16)
Spencer, John F. T.; Spencer, Dorothy M. - Mutagenesis in Yeast (pp. 17-38)
Spencer, John F. T.; Spencer, Dorothy M. - Rare-Mating and Cytoduction in Saccharomyces cerevisiae (pp. 39-44)
Spencer, John F. T.; Spencer, Dorothy M. - Protoplast Fusion in Saccharomyces cerevisiae (pp. 45-50)
Curran, Brendan P. G.; Bugeja, Virginia C. - Meiotic Analysis (pp. 51-58)
Spencer, John F. T.; Spencer, Dorothy M. - Ascus Dissection (pp. 59-68)
Saunders-Singer, Carl - Karyotyping of Yeast Strains by Pulsed-Field Gel Electrophoresis (pp. 69-78)
Johnston, John R. - Chromosomal Localization of Genes Through Pulsed-Field Gel Electrophoresis Techniques (pp. 79-88)
Bignell, Graham R.; Evans, Ivor H. - Isolation, Purification, and Analysis of Nuclear DNA in Yeast Taxonomy (pp. 89-102)
Vaughan-Martini, Ann; Martini, Alessandro - Isolation of Yeast DNA (pp. 103-108)
Piper, Peter - Isolation of Mitochondrial DNA (pp. 109-116)
Bignell, Graham R.; Miller, Angela R. M.; Evans, Ivor H. - Isolation of Yeast Plasma Membranes (pp. 117-122)
Panaretou, Barry; Piper, Peter - The Extraction and Analysis of Sterols from Yeast (pp. 123-132)
Quail, Michael A.; Kelly, Steven L. - Peroxisome Isolation (pp. 133-138)
Distel, Ben; der Leij, Inge van; Kos, Wilko - Transformation of Lithium-Treated Yeast Cells and the Selection of Auxotrophic and Dominant Markers (pp. 139-146)
Mount, Robert C.; Jordan, Bernadette E.; Hadfield, Christopher - Biolistic Transformation of Yeasts (pp. 147-154)
Johnston, Stephen A.; DeVit, Michael J. - Genomic Yeast DNA Clone Banks: Construction and Gene Isolation (pp. 155-188)
Bignell, Graham R.; Evans, Ivor H. - Yeast Colony Hybridization (pp. 189-192)
Kleinman, Matthew John - Determination of Plasmid Copy Number in Yeast (pp. 193-204)
Jordan, Bernadette E.; Mount, Robert C.; Hadfield, Christopher - Determination of Chromosome Ploidy in Yeast (pp. 205-216)
Hadfield, Christopher; Jordan, Bernadette E.; Mount, Robert C. - Chromosome Engineering in Yeast with a Site-Specific Recombination System from a Heterologous Yeast Plasmid (pp. 217-226)
Oshima, Yasuji; Araki, Hiroyuki; Matsuzaki, Hiroaki - Insertional Mutagenesis by Ty Elements in Saccharomyces cerevisiae (pp. 227-238)
Garfinkel, David J. - Reporter Gene Systems for Assaying Gene Expression in Yeast (pp. 239-248)
Mount, Robert C.; Jordan, Bernadette E.; Hadfield, Christopher - Preparation and Use of Yeast Cell-Free Translation Lysate (pp. 249-258)
Hartley, Alan D.; Santos, Manuel A. S.; Colthurst, David R.; Tuite, Michael F. - Electrophoretic Analysis of Yeast Proteins (pp. 259-268)
Grant, Christopher M.; Fitch, Ian T.; Tuite, Michael F. - Preparation of Total RNA (pp. 269-276)
Brown, Alistair J. P. - mRNA Abundance and Half-Life Measurements (pp. 277-296)
Brown, Alistair J. P.; Sagliocco, Francis A. - Polysome Analysis (pp. 297-312)
Sagliocco, Francis A.; Moore, Paul A.; Brown, Alistair J. P. - Induction of Heat Shock Proteins and Thermotolerance (pp. 313-318)
Piper, Peter - Rhodamine B Assay for Estimating Activity of Killer Toxins Permeabilizing Cytoplasmic Membranes (pp. 319-324)
Vondrejs, Vladimir; Palkova, Zdena - Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis (pp. 325-330)
Palkova, Zdena; Vondrejs, Vladimir - Application of Killer Toxins in Stepwise Selection of Hybrids and Cybrids Obtained by Induced Protoplast Fusion (pp. 331-338)
Vondrejs, Vladimir; Palkova, Zdena; Zemanova, Zuzana - Killer Plaque Technique for Selecting Hybrids and Cybrids Obtained by Induced Protoplast Fusion (pp. 339-342)
Palkova, Zdena; Vondrejs, Vladimir - Genotoxicity Testing in Schizosaccharomyces pombe (pp. 343-354)
McAthey, Pamela - Purification and Quantification of Saccharomyces cerevisiae Cytochrome P450 (pp. 355-366)
Stansfield, Ian; Kelly, Steven L. - Calorimetry of Whole Yeast Cells (pp. 367-382)
Ashby, Linda J.; Beezer, Anthony E. - Light Microscopy Methods (pp. 383-390)
Streiblova, Eva; Hasek, Jiri - Fluorescence Microscopy Methods (pp. 391-406)
Hasek, Jiri; Streiblova, Eva - Immunoelectron Microscopy (pp. 407-422)
van Tuinen, Evert-Jan