Library Science

A revised and updated collection readily reproducible techniques for culturing human cells. This edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine? series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Detailed Table of Contents:

1. Establishment and Maintenance of Normal Human Keratinocyte Cultures (pp. 1-8)
   Linge, Claire
2. Cultivation of Normal Human Epidermal Melanocytes (pp. 9-20)
   Hsu, Mei-Yu; Herlyn, Meenhard
3. Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles (pp. 21-32)
   Limat, Alain; Hunziker, Thomas
4. Isolation and Short-Term Culture of Human Epidermal Langerhans? Cells (pp. 33-40)
   Moulon, Corinne
5. Growth and Differentiation of Human Adipose Stromal Cells in Culture (pp. 41-52)
   Strutt, Brenda; Khalil, Wahid; Killinger, Donald
6. Human Fetal Brain Cell Culture (pp. 53-62)
   Mattson, Mark P.
7. Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacological Studies (pp. 63-80)
   Ali-Osman, Francis
8. Cell Culture of Human Brain Tumors on Extracellular Matrices: Methodology and Biological Applications (pp. 81-100)
   Westphal, Manfred; Nausch, Hildegard; Zirkel, Dorothea
9. Isolation and Culture of Human Umbilical Vein Endothelial Cells (pp. 101-110)
   Morgan, David M. L.
10. Human Thymic Epithelial Cell Cultures (pp. 111-120)
    Galy, Anne H. M.
11. Generation and Cloning of Antigen-Specific Human T-Cells (pp. 121-136)
    Yssel, Hans
12. Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1? or Tumor Necrosis Factor-a (pp. 137-152)
    Colinas, Robert J.
13. Human Mononuclear Phagocytes in Tissue Culture (pp. 153-160)
    Keisari, Yona
14. Purification of Peripheral Blood Natural Killer Cells (pp. 161-172)
    Bennett, Ian M.; Perussia, Bite
15. Cystic Fibrosis Airway Epithelial Cell Culture (pp. 173-184)
    Vega, Manuel A.
16. Gene Transfer into Cystic Fibrosis Airway Epithelial Cells (pp. 185-192)
    Vega, Manuel A.
17. Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells (pp. 193-200)
    Vega, Manuel A.
18. Human Tracheal Gland Cells in Primary Culture (pp. 201-216)
    Merten, Marc D.
19. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation (pp. 217-232)
    Goldring, Mary B.
20. Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone (pp. 233-262)
    Gallagher, James A.; Gundle, Roger; Beresford, Jon N.
21. The isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures (pp. 263-276)
    Walsh, Catherine A.; Carron, John A.; Gallagher, James A.
22. In Vitro Cellular Systems for Studying OC Function and Differentiation: Primary OC Cultures and the FLG 29.1 Model (pp. 277-306)
    Aldinucci, Donatella; Quinn, Julian M. W.; Degan, Massimo; Jutbasic, Senka; De luliis, Angela; Improta, Salvatore; Pinto, Antonio; Gattei, Valter
23. Isolation, Purification, and Growth of Human Skeletal Muscle Cells (pp. 307-318)
    Pavlath, Grace K.
24. Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta (pp. 319-334)
    Kirschenlohr, Heide L.; Metcalfe, James C.; Grainger, David J.
25. Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study (pp. 335-344)
    Cavaille, Francoise; Cabrol, Dominique; Ferre, Francoise
26. Primary Culture of Human Antral Endocrine Cells (pp. 345-356)
    Buchan, Alison M. J.
27. Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus (pp. 357-368)
    Accili, Eric A.; Buchan, Alison M. J.
28. Isolation and Culture of Human Hepatocytes (pp. 369-390)
    Bayliss, Martin K.; Skett, Paul
29. Culture of Human Pancreatic Islet Cells (pp. 391-408)
    Sander, Stellan; Eizirik, Decio L.
30. Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules (pp. 409-418)
    Rodilla, Vicente; Hawksworth, Gabrielle M.
31. Glomerular Epithelial and Mesangial Cell Culture and Characterization (pp. 419-430)
    Wilson, Heather M.; Stewart, Keith N.; MacLeod, Alison M.
32. Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tubule Cells (pp. 431-436)
    Todd, John H.; McMartin, Kenneth E.; Sens, Donald A.
33. Culture of Human Renal Medullary Interstitial Cells (pp. 437-446)
    Tisocki, Klara; Hawksworth, Gabrielle M.
34. Culture Technique of Human Pituitary Adenoma Cells (pp. 447-456)
    Kabuto, Masanori
35. Preparation of Human Trophoblast Cells for Culture (pp. 457-464)
    Sooranna, Suren R.
36. Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human Trophoblast Function (pp. 465-476)
    Sooranna, Wren FL; Eaton, Bryan M.
37. Corneal Organ Culture (pp. 477-488)
    Lambert, Robert W.; Richard, Normand R.; Anderson, Janet A.
38. Keratocyte Cell Culture (pp. 489-502)
    Richard, Normand R.; Lambert, Robert W.; Anderson, Janet A.
39. Conjunctiva: Organ and Cell Culture (pp. 503-516)
    Berry, Monica; Ellingham, Roger B.; Corfield, Anthony P.
40. Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium (pp. 517-524)
    Feldman, Eva L.; Del Monte, Monte A.; Stevens, Martin J.; Greene, Douglas A.
41. Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group-Specific Polymerase Chain Reaction (pp. 525-538)
    van Kuppeveld, Frank; van der Logt, Jos; Johansson, Karl-Erik; Melchers, Willem

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