Library Science

This book offers a wide range of readily reproducible methods for sampling, isolating, and culturing all of the major inflammatory cells involved in the pathophysiology of asthma and COPD. The collection of techniques detailed here involve biopsy and sampling of airway liquids such as sputum and exhaled gases such as nitric oxide. Also provided is a full range of methods for the isolation and characterization of cells and for the measurement of the major inflammatory markers, along with protocols for measuring many of the inflammatory mediators and enzymes released during lung inflammation. Among the more advanced techniques are the measurement of exhaled hydrocarbons and F2 isoprostanes, granulocyte pharmacodynamics in whole blood measured by flow cytometry, and tracing mediator trafficking in eosinophils using confocal microscopy. Comprehensive and highly practical, the methods presented in Human Airway Inflammation: Sampling Techniques and Analytical Protocols provide today’s basic and clinical researchers all the major techniques for investigating airway inflammation, and powerfully illuminate many novel targets for emerging drugs.

Authors: Rogers, Duncan F. ; Donnelly, Louise E.

Table of Contents

1. Airway Inflammation and Remodeling in Asthma: Current Concepts (pp. 1-16)
   Holgate, Stephen T.
2. Biopsy Techniques: Optimization for Collection and Preservation (pp. 19-29)
   Saetta, Marina; Turato, Graziella
3. Bronchoalveolar Lavage (BAL): Critical Evaluation of Techniques (pp. 31-59)
   Ward, Chris; Walters, E. Haydn
4. Nasal Secretions and Exudations: Collection and Approaches to Analysis (pp. 61-73)
   Greiff, Lennart; Andersson, Morgan; Persson, Carl G.A.
5. Induced Sputum: Selection Method (pp. 77-91)
   Kelly, Margaret M.; Efthimiadis, Ann; Hargreave, Frederick E.
6. Induced Sputum: Whole Sample (pp. 93-98)
   Keatings, Vera M.; Nightingale, Julia A.
7. Measurement of Exhaled Nitric Oxide and Carbon Monoxide (pp. 99-108)
   Kharitonov, Sergei A.
8. Measurement of Exhaled Hydrocarbons (pp. 109-118)
   Paredi, Paolo; Kharitonov, Sergei A.; Barnes, Peter J.
9. Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide (pp. 119-124)
   de Jongste, Johan C.; Jobsis, Rijn; Raatgeep, H. Rolien
10. Airway Epithelial Cells (Primaries vs Cell Lines) (pp. 127-136)
    Donnelly, Louise E.
11. Isolation and Culture of Human Alveolar Type II Pneumocytes (pp. 137-146)
    Witherden, Ian R.; Tetley, Teresa D.
12. Pulmonary Artery Endothelial Cells (pp. 147-154)
    Seybold, Joachim; Suttorp, Norbert
13. Isolation and Culture of Human Airway Smooth Muscle Cells (pp. 155-160)
    Lazaar, Aili L.; Panettieri, Reynold A.
14. Isolation and Purification of Human Mast Cells and Basophils (pp. 161-176)
    Gibbs, Bernhard F.; Ennis, Madeleine
15. Neutrophils: Collection, Separation, and Activation (pp. 177-189)
    Culpitt, Sarah V.
16. Macrophages: Identification, Separation, and Function (pp. 191-203)
    Poulter, Leonard W.; Burke, C. M.
17. Collection, Separation, and Activation of Human T Lymphocytes (pp. 205-215)
    Sotsios, Yannis; Ward, Stephen G.
18. Eosinophils: Collection, Separation, and Activation (pp. 217-226)
    Koenderman, Leo; van der Linden, Jan; Ulfman, Laurien; Coffer, Paul
19. Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction (pp. 229-246)
    Haddad, El-Bdaoui; Rousell, Jonathan
20. Analysis of Gene Expression: Northern Blotting (pp. 247-261)
    Rousell, Jonathan; Haddad, El-Bdaoui
21. The Technique of In Situ Hybridization (pp. 263-283)
    Ying, Sun; Kay, A. Barry
22. Measurement of Airway Mucin Gene Expression (pp. 285-294)
    Pritchard, Kelly; Smith, Alinka K.; Rogers, Duncan F.
23. Transcription Factors and Inflammatory Gene Regulation: Strategic Approaches (pp. 295-313)
    Newton, Robert; Staples, Karl J.
24. Inflammatory Mediators in Spontaneously Produced Sputum (pp. 317-334)
    Hill, Adam; Gompertz, Simon; Bayley, Darren; Stockley, Robert
25. Flow Cytometry: Measurement of Leukocyte Receptor Expression and Function (pp. 335-344)
    Hartnell, Adele
26. Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry (pp. 345-355)
    Bryan, Shannon A.; Leckie, Margaret J.; Jenkins, Gavin; Barnes, Peter J.; Williams, Timothy J.; Sabroe, Ian; Hansel, Trevor T.
27. Current In Vitro Models of Leukocyte Migration: Methods and Interpretation (pp. 357-365)
    Allport, Jennifer R.; Garcia-Cardena, Guillermo; Lim, Yaw-Chyn; Luscinskas, Francis W.
28. Tracing Intracellular Mediator Storage and Mobilization in Eosinophils (pp. 367-381)
    Mahmudi-Azer, Salahaddin; Lacy, Paige; Moqbel, Redwan
29. Measurement of Metalloproteinases (pp. 383-390)
    Shapiro, Steven D.; Kelley, Diane; Kobayashi, Dale
30. Immunocytochemistry and Immunohistochemistry (pp. 391-398)
    Springer, Jochen; Fischer, Axel
31. The Measurement of Cysteinyl Leukotrienes in Urine (pp. 399-409)
    Westcott, Jay Y.; Wenzel, Sally E.
32. Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid (pp. 411-422)
    Dworski, Ryszard; Sheller, James R.; Christman, Brian W.
33. Quantitative Analysis of F2 Isoprostanes (pp. 423-430)
    Dworski, Ryszard; Christman, Brian W.; Sheller, James R.

This entry was posted on Wednesday, December 27th, 2006 at 5:12 pm and is filed under Library. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.