Library Science

Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.

Ed. Aguilar, Marie-Isabel

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Table of Contents

1. HPLC of Peptides and Proteins: Basic Theory and Methodology (pp. 3-8)
   Aguilar, Marie-Isabel
2. Reversed-Phase High-Performance Liquid Chromatography (pp. 9-22)
   Aguilar, Marie-Isabel
3. Ion-Exchange Chromatography (pp. 23-44)
   Stanton, Peter
4. High-Performance Hydrophobic Interaction Chromatography (pp. 45-54)
   Benedek, Kalman
5. Gel Filtration Chromatography (pp. 55-74)
   Stanton, Peter
6. Hydrophilic Interaction Chromatography (pp. 75-88)
   Lindner, Herbert; Helliger, Wilfried
7. Immobilized Metal Ion Affinity Chromatography of Proteins (pp. 89-102)
   Zachariou, Michael
8. Immunoaffinity Chromatography of Proteins (pp. 103-110)
   Gallant, Stuart R.
9. Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins (pp. 111-140)
   Shen, Tun-Li; Noon, Kathleen R.
10. Capillary Separations (pp. 143-164)
    Carrascal, Montserrat; Abian, Joaquin
11. Micropreparative HPLC of Peptides and Proteins (pp. 165-176)
    Nice, Edouard C.; Aguilar, Marie-Isabel
12. Multidimensional HPLC Purification of Proteins (pp. 177-182)
    Nice, Edouard C.; Aguilar, Marie-Isabel
13. Analytical High-Performance Liquid Chromatography (pp. 183-190)
    Benedek, Kalman
14. Prep/Semiprep Separations of Peptides (pp. 191-210)
    Scanlon, Denis B.; Finlayson, James
15. Large-Scale Protein Chromatography (pp. 211-224)
    Bertolini, Joseph; Gomme, Peter; Thomas, Patrick
16. Proteolytic Peptide Mapping (pp. 227-244)
    Hojrup, Peter
17. Mass Spectrometric Characterization of Posttranslationally Modified Proteins– Phosphorylation (pp. 245-262)
    Larsen, Martin R.
18. Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry (pp. 263-274)
    Kawasaki, Nana; Ohta, Miyako; Itoh, Satsuki; Hayakawa, Takao
19. HPLC in the Analysis of Peptide Metabolism (pp. 275-290)
    Lew, Rebecca A.
20. Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells (pp. 291-306)
    Purcell, Anthony W.
21. Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques (pp. 307-322)
    Wilson, David; Alewood, Paul
22. HPLC and Mass Spectrometry of Intrinsic Membrane Proteins (pp. 323-340)
    Whitelegge, Julian P.
23. IgG Purification (pp. 341-350)
    Powell, Maree S.; Wines, Bruce D.
24. DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions (pp. 351-364)
    Buckle, Malcolm
25. Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC (pp. 365-376)
    Gomez-Ramirez, Jordi; Blanco, Isaac; Ortiz, Jordi
26. Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry (pp. 377-386)
    Aubagnac, Jean-Louis; Enjalbal, Christine; Martinez, Jean; Sanchez, Pierre; Subra, Gilles
27. Proteome Analysis (pp. 387-400)
    Powell, Matthew J.; Timperman, Aaron T.

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