Library Science

For volume 1: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Authors: Hamatake, Robert K. ; Lau, Johnson Y. N.

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Table of Contents

1. Overview of Commercial HBV Assay Systems (pp. 3-14)
   Zeuzem, Stefan
2. Detection of HBV DNA in Serum Using a PCR-Based Assay (pp. 15-20)
   Chung, Hau Tim
3. Detection of HBV DNA by Oligonucleotide Probing (pp. 21-28)
   Lin, Hsiang Ju
4. Detection of HBV RNA in Serum of Patients (pp. 29-40)
   Zhang, Wei; Hacker, Hans Jorg; Mildenberger, Maria; Su, Qin; Schroder, Claus H.
5. Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR (pp. 41-50)
   Bowden, Scott; Jackson, Kathy; Littlejohn, Margaret; Locarnini, Stephen
6. In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections (pp. 51-56)
   Lai, Vicky C. H.; Lau, Johnson Y. N.
7. Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis (pp. 57-64)
   Gerlich, Wolfram H.; Wend, Ulrike; Glebe, Dieter
8. In Situ Detection of Hepatitis B Viral Antigens : An Immunohistochemical Approach (pp. 65-70)
   Fang, Jane W.S.; Lau, Johnson Y.N.
9. Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting (pp. 71-84)
   Liu, Jie; Feitelson, Mark A.
10. Detection of Serum HDV RNA by RT-PCR (pp. 85-94)
    Smedile, Antonina; Niro, Maria Grazia; Rizzetto, Mario
11. Nonradioisotopic In Situ Hybridization for HDV RNA (pp. 95-98)
    Negro, Francesco
12. Detection and Characterization of Small and Large HDV Antigens (pp. 99-106)
    Macnaughton, Thomas B.; Lai, Michael M.C.
13. Immunohistochemical Detection of Hepatitis Delta Antigen (pp. 107-112)
    Abid, Karim; Negro, Francesco
14. HBV Vaccine-Escape Variants (pp. 115-124)
    Basuni, Ashraf A.; Carman, William F.
15. HBV Genotyping and Analysis for Unique Mutations (pp. 125-150)
    Ayres, Anna; Locarnini, Stephen; Bartholomeusz, Angeline
16. A One-Filter???Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus (pp. 151-164)
    Shih, Chiaho; Yuan, Ta-Tung Thomas
17. Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion (pp. 165-174)
    Shih, Chiaho; Tai, Pei-Ching
18. Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay (pp. 175-186)
    De Gendt, Sija; Shapiro, Fred; Juras, Jelena; Van Assche, Els; Maertens, Geert; Sablon, Erwin
19. Processing of Hepatitis B Virus Surface Proteins (pp. 189-198)
    Bruss, Volker
20. Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus (pp. 199-212)
    Urban, Stephan
21. Nucleoprotein Transport of HBV Capsid Particles (pp. 213-226)
    Kann, Michael
22. Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cells (pp. 227-234)
    Li, Jie; Zheng, Yanyan; Choi, Jinah; Ou, Jing-hsiung
23. Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions (pp. 235-246)
    Zoulim, Fabien; Tong, ShuPing; Trepo, Christian
24. A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein (pp. 247-258)
    Shih, Chiaho; Yuan, Ta-Tung Thomas
25. Studying DHBV Polymerase by In Vitro Transcription and Translation (pp. 259-270)
    Hu, Jianming
26. Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System (pp. 271-280)
    Lott, Lisa; Notvall, Lena; Lanford, Robert E.
27. Localization of Duck Hepatitis B Virus Polymerase Within Cells (pp. 281-294)
    Yao, Ermei; Tavis, John E.
28. Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice (pp. 295-302)
    Xu, Zhenming; Ou, Jing-hsiung
29. Transcriptional Control of Hepatitis B Virus (pp. 303-314)
    Raney, Anneke K.
30. In Vitro Reconstitution of e-Dependent Duck Hepatitis B Virus Replication Initiation (pp. 315-326)
    Beck, Jurgen; Nassal, Michael
31. Hepatitis B Viruses : A Triple Threat for Malignant Transformation of Hepatocytes (pp. 327-338)
    Rogler, Charles E.

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