Library Science

A collection of readily reproducible experimental and clinical techniques for studying the molecular basis of hemoglobin disorders. These step-by-step methods utilize a wide variety of the latest analytical techniques, including x-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin.

Authors: Nagel, Ronald L

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Table of Contents

1. X-ray Crystallography of Hemoglobins (pp. 001-019)
   Safo, Martin K.; Abraham, Donald J.
2. Analysis of Hemoglobins and Globin Chains by High-Performance Liquid Chromatography (pp. 021-029)
   Wajcman, Henri
3. Purification and Molecular Analysis of Hemoglobin by High-Performance Liquid Chromatography (pp. 031-047)
   Manjula, Belur N.; Acharya, Seetharama A.
4. Oxygen Equilibrium Measurements of Human Red Blood Cells (pp. 049-064)
   Kister, Jean; Wajcman, Henri
5. Measurement of Rate Constants for Reactions of O2, CO, and NO with Hemoglobin (pp. 065-091)
   Olson, John S.; Foley, Erin W.; Maillett, David H.; Paster, Eden V.
6. Electrophoretic Methods for Study of Hemoglobins (pp. 093-100)
   Wajcman, Henri
7. DNA Diagnosis of Hemoglobin Mutations (pp. 101-116)
   Old, John M.
8. Methods for Analysis of Prenatal Diagnosis (pp. 117-131)
   Old, John M.
9. Hemoglobin Fluorescence (pp. 133-154)
   Hirsch, Rhoda Elison
10. Nucleation and Crystal Growth of Hemoglobins: The Case of HbC (pp. 155-176)
    Vekilov, Peter G.; Feeling-Taylor, Angela; Hirsch, Rhoda Elison
11. Semisynthesis of Hemoglobin (pp. 177-193)
    Acharya, Seetharama A.; Srinivasulu, Sonati
12. ?÷-Globin-like Gene Cluster Haplotypes in Hemoglobinopathies (pp. 195-211)
    Muralitharan, Shanmugakonar; Krishnamoorthy, Rajagopal; Nagel, Ronald L.
13. Transgenic Mice and Hemoglobinopathies (pp. 213-241)
    Fabry, Mary E.; Bouhassira, Eric E.; Suzuka, Sandra M.; Nagel, Ronald L.
14. Recombinant Single Globin-Chain Expression and Purification (pp. 243-250)
    Adachi, Kazuhiko
15. Nuclear Magnetic Resonance of Hemoglobins (pp. 251-269)
    Lukin, Jonathan A.; Ho, Chien
16. Solubility Measurement of the Sickle Polymer (pp. 271-287)
    Fabry, Mary E.; Acharya, Seetharama A.; Suzuka, Sandra M.; Nagel, Ronald L.

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